Abstract
In this study, a commercial electronic nose system was used to detect contamination of Salmonella bacteria. Odors from growth media contaminated with Salmonella typhimurium, Salmonella enteritidis, or Escherichia coli were collected and analyzed to evaluate a possibility of rapid detection of pathogen. Odor chromatograph showed that S. typhimurium, S. enteritidis, and E. coli had 7,6, and 9 main peaks, respectively. Retention time and intensity of the peaks were distinct for different bacteria species. Principal component analysis (PCA) were also performed to clarify odor differences. Analysis results showed that the odors for uncontaminated growth medium were differently grouped from the odors of contaminated one. The odor from the bacteria growth identified with two principal components, PC 1 and PC2. In PCA figures, odor groups were moved from left to right of PC 1 with elapse of the bacteria growth time. The electronic nose system could detect odors of S. typhimurium, S. enteritidis, E. coli when their concentration were $1.85\times10^6\;cfu/g,\;2.25\times10^6\;cfu/g,\;and\;1.8\times10^5 cfu/g$, respectively.
