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The Pharmaceutical Society of Korea (대한약학회)
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Sensitization of Vanilloid Receptor Involves an Increase in the Phosphorylated Form of the Channel

  • Lee Soon-Youl (Department of Genomic Engineering, Genetic Informatics Center, GRRC) ;
  • Lee Jae-Hag (Department of Food and Nutrition, Seoil College) ;
  • Kang Kwon Kyoo (Department of Horticulture, Hankyong National University) ;
  • Hwang Sue-Yun (Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Choi Kang Duk (Graduate School of Bio & Information Technology, Hankyong National University) ;
  • Oh Uhtaek (The Sensory Research Center, National Creative Research Initiatives, College of Pharmacy, Seoul National University)
  • Published : 2005.04.01
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Abstract

A vanilloid receptor (VR1, now known as TRPV1) is an ion channel activated by vanilloids, including capsaicin (CAP) and resiniferatoxin (RTX), which are pungent ingredients of plants. Putative endogenous activators (anandamide and metabolites of arachidonic acid) are weak activators of VR1 compared to capsaicin and RTX, and the concentrations of the physiological condition of those activators are not sufficient to induce significant activation of VR1. One way to overcome the weak activation of endogenous activators would be the sensitization of VR1, with the phosphorylation of the channel being one possibility. The phosphorylation of VR1 by several kinases has been reported, mostly by indirect evidence. Here, using an in vivo phosphorylation method, the VR1 channel was shown to be sensitized by phosphorylation of the channel itself by multiple pathways involving PKA, PKC and acid. Also, in sensitizing VR1, BK appeared to show activation of PKC for the sensitization of VR1 by phosphorylation of the channel.

Keywords

References

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