Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Influence of Exogenous Abscisic Acid Concentration on the Coloration of 'Kyoho' Grapes.

Abscisic acid의 처리농도가 포도 '거봉'의 착색에 미치는 영향

  • Han Dong Hyeon (Division of Bioscience and Technology, Korea University)
  • 한동현 (고려대학교 생명산업과학부)
  • Published : 2005.04.01
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Abstract

This experiment was carried to investigate the optimum concentration of abscisic acid (ABA) treatment for enhancing fruit coloration of 'Kyoho' grapes. Cluster and berry weights showed a tendency that increased in proportion to concentration of ABA treatment, but were not significant in all treatments. Also, soluble solids and titratable acidity were not significant during fruit development in all treatments. L-phenylalanine ammonia-lyase (PAL) activity showed a tendency that decreased after rapidly increased in all treatments, and was the highest in 1000 mg/l ABA treatment at final harvest. Anthocyanin and total phenolics contents were high in proportion to ABA treatment concentration, and anthocyanin content in 1000 mg/l ABA treatment was 2.5 folds of that in control. Fructose and glucose as soluble sugars were detected, but sucrose was not detected. Both fructose and glucose contents increased during fruit development, but showed little difference in all treatments.

본 실험은 착색 불안정 때문에 재배적 어려움이 있는 '거봉'포도를 이용하여 변색기에 ABA를 농도별로 처리하여 봄으로써 착색증진을 위한 최적의 처리 농도를 구명하고자 실시하여 다음과 같은 결과를 얻었다. ABA의 처리 농도에 따라 과방중량과 과립중량은 약간 증가하는 경향을 나타내었으나, 유의차가 인정되지 않았다. 가용성 고형물과 적정산 함량 역시 생장기간 내내 처리구간에 유의차가 인정되지 않았다. PAL 효소의 활성은 각 처리구 공히 처리후 급속히 증가 하다가 감소하는 경향을 보였는데, ABA 처리농도가 높으면 높을수록 증감의 폭은 더욱 커졌다. 또한 처리농도가 높을수록 최종수확기에 가서도 다른 처리구에 비해 높은 수준을 유지하였다. 안토시아닌 함량은 모든 처리구에서 생육기간 동안 계속 증가하는 경향을 보였고, ABA 처리농도가 높아짐에 따라 더 높은 함량을 나타내었는데, 특히 ABA 1000 mg/L 처리구는 최종수확기에 무처리구에 비해 2.5배 이상의 함량을 나타내었다. 과피의 전페놀 함량은 안토시아닌 함량과 마찬가지로 모든 처리구에서 생육기간 내내 증가하는 경향을 나타내었고, ABA의 처리농도가 높아짐에 따라 더 높은 함량을 나타내었다. 가용성당 함량은 과당과 포도당만이 검출되었으며, 자당은 전혀 검출되지 않았다. 과당과 포도당 함량 역시 변색기 이후 계속 증가하는 경향을 보였으며, 처리구간에 큰 차이는 보이지 않았다.

Keywords

References

  1. Arakawa, O. 1988. Photoregulation of anthocyanin synthesis in apple fruit under UV -B and red light. Plant Cell Physiol. 29, 1385-1389
  2. Budini, R., D. Tonelli and S. Girotti. 1980. Analysis of total phenols using the prussian blue method. J. Agric. Food. Chem. 28, 1236-1238 https://doi.org/10.1021/jf60232a056
  3. Camm, E. L. and G. H. Neil Towers. 1973. Phenylalanine ammonia-lyase. Phytochem. 12, 961-973 https://doi.org/10.1016/0031-9422(73)85001-0
  4. Chandler, S. F. and J. H. Dodds. 1983. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasodine in callus cultures of Solanum laciniatum. Plant Cell Rep. 2, 205-208 https://doi.org/10.1007/BF00270105
  5. Coombe, B. G. and C. R. Hale. 1973. The hormone content of ripening grape berries and the effects of growth substance treatments. Plant Physiol. 51, 629-634 https://doi.org/10.1104/pp.51.4.629
  6. Craker, L. E. and P. J. Wetherbee. 1973. Ethylene, light, and anthocyanin synthesis. Plant Physiol. 51, 436-438 https://doi.org/10.1104/pp.51.3.436
  7. Hardy, P. J. 1968. Metabolism of sugars and organic acids in immature grape berries. Plant Physiol. 43, 224-228 https://doi.org/10.1104/pp.43.2.224
  8. Kataoka, I., A. Sugiura, N. Utsunomiya and T. Tomana. 1982. Effect of abscisic acid and defoliation on anthocyanin accumulation in Kyoho grapes (Vitis vinifera L.xV. labruscana Bailey). Vitis 21, 325-332
  9. Kataoka, I., Y. Kubo, A. Sugiura and T. Tomana. 1983. Changes in L-phenylalanine ammonia-lyase activity and anthocyanin synthesis during berry ripening of three grape cultivars. J. Japan. Soc. Hort. Sci. 52, 273-279 https://doi.org/10.2503/jjshs.52.273
  10. Kliewer, W. M. 1964. Influence of environment on metabolism of organic acids and carbohydrates in Vitis vinifera. I. Temperature. Plant Physiol. 39, 869-880 https://doi.org/10.1104/pp.39.6.869
  11. Kliewer, W. M. 1966. Sugars and organic acids of Vitis vinifera. Plant Physiol. 41, 923-931 https://doi.org/10.1104/pp.41.6.923
  12. Kliewer, W. M. 1970. Effect of day temperature and light intensity on coloration of Vitis vinifera L. grapes. J. Amer. Soc. Hort. Sci. 95, 693-697
  13. Kliewer, W. M. and L. A. Lider. 1970. Effects of day temperature and light intensity on growth and composition of Vitis vinifera L. fruits. J. Amer. Soc. Hort. Sci. 95, 766-769
  14. Lee, J. C, T. Tomana, N. Utsunomiya and I. Kataoka. 1979. Physiological study on the anthocyanin development in grape. I. Effect of fruit temperature on the anthocyanin development in 'Kyoho' grape. J. Kor. Soc. Hort. Sci. 20, 55-65
  15. Lee, J. C. and T. Tomana. 1981. Physiological study on the coloration in grape. III. Effect of fruit temperature on the changes of total sugar, total phenol and abscisic acid in 'Delaware' and 'Muscat Bailey A' grape berries. J. Kor. Soc. Hort. Sci. 22, 283-288
  16. Lee, S. M, D. H. Han, C. H. Lee and S. B. Kim. 1996. Effects of ABA and kinetin treatments on the coloration and quality of 'Campbell Early' and 'Black Olympia' grapes. J. Kor. Soc. Hort. Sci. 37, 263-268
  17. Mancinelli, A. L. and I. Rabino. 1984. Photoregulation of anthocyanin synthesis X. Dependence on photosynthesis of high irradiance response anthocyanin synthesis in Brassica oleracea leaf disks and Spirodela polyrrhiza. Plant Cell Physiol. 25, 1153-1160
  18. Miura, H., M. Shimizu, A. Tazuke and M. Iwata. 1989. Effect of monochromatic light on anthocyanin content in seedlings of Benitade (Polygonum hydropiper L.). J. Japan. Soc. Hort. Sci. 58, 123-129 https://doi.org/10.2503/jjshs.58.123
  19. Richmond, M. L., S. C. C. Brandao, J. Ian Gray, P. Markakis and C. M. Stine. 1981. Analysis of simple sugars and sorbitol in fruit by high-performance liquid chromatography. J. Agric. Food Chem. 29, 4-7 https://doi.org/10.1021/jf00103a002
  20. Taiz, L. and E. Zeiger. 1991. Plant physiology. pp 473-489, The Benjamin/Cummings Publishing Company, Inc. California

Cited by

  1. Fruit Color Improvement by ABA Treatment and Determination of Harvesting Time in 'Hongisul' Grapes vol.30, pp.3, 2012, https://doi.org/10.7235/hort.2012.12064