Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Immuno-Electronmicroscopic Studies on the Expression of Tenascin in the Synovial Cells of Human Knee Joint

퇴행성관절염의 사람 무릎관절 윤활막에서 Tenascin 발현에 대한 면역전자현미경적 연구

  • Lim, Hyoung-Soo (Department of Anatomy, College of Medicine, Seoul National University) ;
  • Choi, Hee-Joon (Department of Anatomy, College of Medicine, Seoul National University) ;
  • Lee, Se-Jeong (Department of Anatomy, College of Medicine, Seoul National University) ;
  • Hwang, Douk-Ho (Department of Anatomy, College of Medicine, Seoul National University)
  • 임형수 (서울대학교 의과대학 해부학교실) ;
  • 최희준 (서울대학교 의과대학 해부학교실) ;
  • 이세정 (서울대학교 의과대학 해부학교실) ;
  • 황덕호 (서울대학교 의과대학 해부학교실)
  • Published : 2005.06.01
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Abstract

To observe the morphological changes and the expression of tenascin in synovium of human knee joint, between normal condition and degenerative arthritis, were processed by immunoelectron microscopic method. The results were summarized as follows. 1. In degenerative arthritis, the hyperplasia of synovial membrane was characterized by the increase of cell number in secretory synovial cells. 2. In normal condition, there was no marking of the immuno-gold for tenascin in synovial membrane. 3. In degenerative arthritis, the immuno-gold for tenascin were observed in endoplasmic reticulum of secretory synovial cells and extracellular matrix of synovial layer. On the basis of above findings, the hyperplasia of synovial membrane and the pathologic processes may be concerned with the increase of number of secretory synovial cells and of expression of tenascin, in degenerative arthritis.

사람 무릎관절의 윤활막을 대상으로 정상과 퇴행성관절염 시의 윤활막의 변화와 tenascin의 발현을 면역전자현미경적 방법으로 비교, 관찰한 결과 다음과 같은 결과를 얻을 수 있었다. 1. 정상의 윤활막과 비교하여 퇴행성관절염 시에 윤활막이 두터워지는 것은 주로 윤활분비세포의 수적인 증가에 의한 것임을 알 수 있었다. 2. Tenascin에 대한 면역금의 관찰 결과 정상의 윤활막을 구성하는 세포에서는 면역금의 표지가 관찰되지 않았다. 3. 퇴행성관절염 시 윤활막의 윤활분비세포 과립세포질 세망에서 면역금의 표지가 관찰되어 tenascin의 분비세포가 윤활분비세포임을 확인할 수 있으며, 윤활세포 사이의 바탕질의 아교섬유에서도 tenascin의 표지가 관찰되었다. 이상의 결과를 종합해 보면, 퇴행성관절염 시에 윤활막이 두터워지는 것과 병변의 진행과정에, 윤활분비세포의 수가 증가와 tenascin의 발현 증가가 관련성이 있는 것으로 생각된다.

Keywords

References

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