Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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Hyperacute Radiation Effect on Cerebral Cortex after Local Gamma-irradiation in the Rat Brain

단일 국소방사선 조사 후 백서 대뇌 피질의 초급성기 변화에 대한 연구

  • Kang, Shin-Hyuk (Department of Neurosurgery, College of Medicine, Korea University) ;
  • Chung, Yong-Gu (Department of Neurosurgery, College of Medicine, Korea University) ;
  • Kim, Han-Kyum (Department of Pathology, College of Medicine, Korea University) ;
  • Kim, Chul-Yong (Department of Radiooncology, College of Medicine, Korea University) ;
  • Lee, Hoon-Kap (Department of Neurosurgery, College of Medicine, Korea University)
  • 강신혁 (고려대학교 의과대학 신경외과학교실) ;
  • 정용구 (고려대학교 의과대학 신경외과학교실) ;
  • 김한겸 (고려대학교 의과대학 병리학교실) ;
  • 김철용 (고려대학교 의과대학 치료방사선학교실) ;
  • 이훈갑 (고려대학교 의과대학 신경외과학교실)
  • Published : 2005.05.28
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Abstract

Objective: We investigated the morphologic changes within 24 hours after a single ${\gamma}$-irradiation in the rat brain. Methods: Forty Sprague-Dawley rats were used. After a burr hole trephination on right parietal area, cerebral hemisphere was irradiated with 2Gy and 5Gy using iridium-192($^{192}Ir$), respectively. The effect was assessed at 4, 8, 12 and 24 hours after irradiation. The histological changes were scored following the detection of edema or disarray severity. TUNEL-positive cells exhibiting apoptotic morphology were counted in irradiated region. Results: Cortical edema and disarray were initially showed at 4 or 8 hour and almost all defined at 24 hour after irradiation. And the injury was wedge shape. TUNEL-positive cells were minimal at 8 hour after irradiation as the number of positive cells were $2.6{\pm}5.27$(n=5) after 2Gy, and $0.8{\pm}0.84$(n=5) after 5Gy. But, the number of apoptotic cells were increased markedly to $60{\pm}6.24$ at 12 hour after 2Gy and to $104{\pm}19.7$ at 24 hour after 5Gy. Conclusion: There were prominent morphologic changes immediately after ${\gamma}$-irradiation. And, apoptosis was increased according to the time period. These findings implicate that brain irradiation induces rapid apoptotic change, which may play an important role in the pathogenesis of radiation-induced pathologic conditions.

Keywords

References

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