Journal of Acupuncture Research
- 제22권1호
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- Pages.155-164
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
반하(半夏) 약침액(藥鍼液)이 사람 기관지 상피세포의 TARC 분비에 미치는 효과
Effect of Pinelliae Rhizoma Herbal Acupuncture on the Release of Thymus and Activation-Regulated Chemokine(TARC) in Human Bronchial Epithelial Cell
- 홍재화 (대구한의대학교 한의과대학 침구학교실) ;
- 서정철 (대구한의대학교 한의과대학 침구학교실) ;
- 임성철 (대구한의대학교 한의과대학 침구학교실) ;
- 정태영 (대구한의대학교 한의과대학 침구학교실) ;
- 한상원 (대구한의대학교 한의과대학 침구학교실)
- Hong, Jae-hwa (Department of Acupuncture & Moxibustion, College of Oriental Medicine Daegu Haany University) ;
- Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine Daegu Haany University) ;
- Lim, Seong-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine Daegu Haany University) ;
- Jung, Tae-young (Department of Acupuncture & Moxibustion, College of Oriental Medicine Daegu Haany University) ;
- Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine Daegu Haany University)
- 투고 : 2005.01.18
- 심사 : 2005.01.27
- 발행 : 2005.02.20
Abstract
사람 기관지 상피세포에 알러지 환경을 유발 하고자 사이토카인을 처리하여 TARC의 분비를 유도하고, 이 케모카인 분비에 반하(半夏) 약쇄액(藥鎖液)이 미치는 효과를 실험한 결과 다음과 같은 결론을 얻었다. 1. 사람의 기관지 상피 세포주에 각각 IL-4, TNF-
Objective : Chemokines are important for the recruitment of leukocytes, which is essential in host defense to the sites of infection. The thymus and activation-regulated chemokine (TARC) is a CC chemokine which potentially plays a role via a paracrine mechanism in the development of allergic respiratory diseases. Methods : The objective of this study is to investigate the effect of Pinelliae Rhizoma Herbal Acupuncture Solution (PHS) on the secretion of TARC of human bronchial epithelial cell. Methods : Enzyme-linked immunosorbent assay (ELISA) was performed to detect the secretion of TARC. The cytotoxicity was measured by MTT assay. Results : PHS significantly inhibited the secretion of TARC with a dose-dependant manner. The effective dosage did not have the cytotoxicity on human bronchial epithelial cell by MTT assay. Conclusion : Results of our study imply that PHS would play an important role in modulation of TARC in Human bronchial epithelial cells.
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