Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Micro-propagation Factors Essential for Mass Production of Synthetic Seeds in Banana

  • Hassanein A. M. (Genetics lab., Faculty of Science, South Valley University) ;
  • Ibrahiem I. A. (Faculty of Agriculture, Cairo University) ;
  • Galal A. A. (Genetics lab., Faculty of Science, South Valley University) ;
  • Salem J. M. M. (Genetics lab., Faculty of Science, South Valley University)
  • Published : 2005.09.01
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Abstract

This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

Keywords

References

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