Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Increasement of Secondary Metabolites and Antioxidative Activity in Panax ginseng Adventitious Root by Methyl Jasmonate

Methyl jasmonate 처리에 의한 인삼 (Panax ginseng C.A. Meyer) 부정근의 이차대사산물 및 항산화활성 증가

  • Lim Soon (Laboratory of Plant Cell Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KIRBB)) ;
  • Bae Ki-Hwa (Korea Ginseng Institute, Chung-Ang University) ;
  • Shin Cha-Gyun (Korea Ginseng Institute, Chung-Ang University) ;
  • Kim Yoon-Young (Korea Ginseng Institute, Chung-Ang University) ;
  • Kim Yun-Soo (Korea Ginseng Institute, Chung-Ang University)
  • 임순 (한국생명공학연구원 식물세포공학연구실) ;
  • 배기화 (중앙대학교 인삼산업연구소) ;
  • 신차균 (중앙대학교 인삼산업연구소) ;
  • 김윤영 (중앙대학교 인삼산업연구소) ;
  • 김윤수 (중앙대학교 인삼산업연구소)
  • Published : 2005.09.01
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Abstract

This study was initiated to investigate the impacts of methyl jasmonate (MeJA) on adventitious root growth of Panax ginseng, the production of secondary metabolites, such as ginsenosides and phenolic compounds, and antioxidative activity. Among various concentrations of MeJA, $100\;{\mu}M$ MeJA increased the ginsenosides accumulation to 26.6 mg/g dry wt, about 8 times higher than the control in ginseng adventitious roots (GAR). In addition, $50\;{\mu}M$ MeJA increased the accumulation of phenolic compounds to 0.38 mg/g dry wt, about 3 times higher than control in GAR. This MeJA treatment was more effective in conditioned medium (CM) which obtained in bioreactor after 40 days of culture than in fresh medium (FM). Treatment of $100\;{\mu}M$ MeJA in CM increased the accumulation of ginsenosides (1.7 times) and phenolic compounds (1.2 times) more than in FM, respectively. Consequently, these high accumulation of ginsenosides and phenolic compounds by MeJA led to increase the antioxidative activities expressed to the DPPH scavenging activity (over $78.3\%$). The DPPH scavenging activity in control was $45.5\%$.

인삼부정근 배양에서 MeJA 처리가 부정근내 진세노사이드와 페놀화합물의 생산에 미치는 영향과 이러한 이차대사 산물의 증가에 따른 인삼부정근의 항산화활성의 효과를 조사하였다. 다양한 농도의 MeJA를 인삼부정근에 처리한 결과, $100\;{\mu}M$ MeJA에서 부정근내 진세노사이드의 생산은 26.6 mg/g dry wt로 대조구보다 약 8배 이상 생산하는 것으로 나타났다. 하지만, MeJA의 처리는 부정근의 생장을 감소시키는 것으로 나타났다. 페놀화합물의 생산 역시 MeJA처리에 의하여 증가되는 경향을 나타냈으며 $50\;{\mu}M$ MeJA에서 0.38 mg/g dry wt로 대조구에 비하여 약 3배 이상 증가한 것으로 나타났다. 이러한 MeJA의 처리효과는 조건배지 (CM)를 이용하는 것이 신선배지 (FM)를 이용하는 것보다 유리한 것으로 나타났다. 배지성분이 결핍된 조건배지에서의 진세노사이드 생산은 신선배지에서 보다 약 1.7배가 증가한 것으로 나타났으며, 페놀화합물 역시 조건배지에서약 1.2배가 증가한 것으로 나타났다. 이러한 MeJA 처리에 의한 진세노사이드와 페놀화합물의 증가는 결과적으로 인삼부정근의 항상화활성을 무처리구인 대조구에 비해서 약 72%이상 증가시키는 것으로 나타났다.

Keywords

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