Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Inhibitory Effects of GC, an Extract from Herbs, on $TNF-{\alpha}/IL-1{\beta}$-induced Activation of Human Fibroblast-like Sinoviocytes

계혈등복합방(GC)의 $TNF-\alpha$와 $IL-1{\beta}$로 유도된 인간 섬유아세포양 활막 세포 활성화 억제 작용

  • Jang Kwang-Ho (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Jin Mi-Rim (Department of Pathology, College of Oriental Medicine, Daejeon University) ;
  • Park Hee-Ok (Department of Food and Drug, Chosun University) ;
  • Kim Dong-Hee (Department of Pathology, College of Oriental Medicine, Daejeon University)
  • 장광호 (대전대학교 한의과대학 병리학교실) ;
  • 진미림 (대전대학교 한의과대학 병리학교실) ;
  • 박희옥 (조선대학교 식품의약학과) ;
  • 김동희 (대전대학교 한의과대학 병리학교실)
  • Published : 2005.10.01
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Abstract

Based on traditional medicine theories, GC, an extract from 5 herbs, has been formulated and prescribed for the treatment of human rheumatoid arthritis(hRA) for many years. The present studies was done to investigate whether GC has inhibitory effects on activation of fibroblast-like sinoviocytes isolated from a RA patient. In tumor necrosis factor-${\alpha}(TNF-{\alpha}$)/ interleukin-IL-$1{\beta}$(IL-$1{\beta}$) treated human sinoviocytes, the mRNA expression of molecular indicators related to pathologic changes of the sinoviocytes were examined using quantitative real-time PCR. The treatment of GC($10{\mu}g/ml$) significantly suppressed the expression of proinflammatory cytokines and chemokines such as $TNF-{\alpha}$, IL-6 and IL-8 compared with the control, but not $IL-1{\beta}$, The mRNA level of intracellular adhesion molecule-1 (ICAM-1) which is known to increase in the activated sinoviocytes of RA patients, was slightly decreased by GC. The expression of NOS-II was considerably reduced, which was accompanied by a decrease in the production of nitric oxide(NO). Furthermore, GC dramatically raised the mRNA levels of tissue inhibitors of matrix metalloproteinase-1 (TIMP-1), while those of matrix metalloproteinase-3 were significantly lowered. Taken together, these data suggested that GC might suppress the activation of sinoviocytes in hRA.

Keywords

References

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