Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of the Ethanol Extract from Steamed Roots of Rehmannia Glutinosa on the Antioxidant Enzyme Activities in HEI-OC1 Auditory Cells

숙지황 에탄올 추출물이 HEI-OC1 세포의 항산화 효소 활성에 미치는 영향

  • Yu Hyeon-Hee (Department of Food and Nutrition, Kunsan National University) ;
  • Kim Yeon-Hwa (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University) ;
  • Jung Su-Young (Department of Food and Nutrition, Wonkwang University) ;
  • Shin Mee-Kyung (Department of Food and Nutrition, Wonkwang University) ;
  • Park Rae-Kil (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University) ;
  • So Hong-Seob (Department of Microbiology & Vestibulocochlear Research Center, School of Medicine, Wonkwang University) ;
  • Jeon Byung-Hun (Department of Oriental Pathology, College of Oriental Medicine, Wonkwang University) ;
  • You Yong-Ouk (Department of Oral Biochemistry & Vestibulocochlear Research Center, School of Dentistry, Wonkwang University)
  • 유현희 (군산대학교 식품영양학과) ;
  • 김연화 (원광대학교 치과대학 구강생화학교실.VCRC) ;
  • 정수영 (원광대학교 식품영양학과) ;
  • 신미경 (원광대학교 식품영양학과) ;
  • 박래길 (원광대학교 의과대학 미생물학과.VCRC) ;
  • 소홍섭 (원광대학교 의과대학 미생물학과.VCRC) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 유용욱 (원광대학교 치과대학 구강생화학교실.VCRC)
  • Published : 2005.12.01
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Abstract

A mechanism of hair cell damage caused by noise and ototoxic agents is mediated through generation of free radicals and reactive oxygen species (ROS). It is known that most of animals have defense systems to protect against ROS, and the cochlea of inner ear in animals also has ROS defense systems including several antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR), and glutathione (GSH), which efficiently detoxifying ROS generated under normal condition. Steamed roots of Rehmannia glutinosa have been traditionally used in Oriental medicine for the treatment of auditory disease such as tinnitus, vertigo, and hearing loss as well as inflammatory diseases, hectic fever, night sweat, and headache. In the present study, we showed that the ethanol extract from steamed roots of R. giutinosa (ESRG) increased the antioxidant enzymes such as SOD, CAT, GPX, and GR activities and GSH level in HEI-OC1 auditory cells. This extract itself did not show any significant cytotoxicity up to $50{\mu}g/ml$. Our results further support the view that ESRG is promising sources of potential antioxidants. Future studies will be aimed at investigating the effects of ESRG on the regulation of cellular mechanisms and isolating and identifying the substances responsible for the regulation of antioxidant enzyme system from the plant extracts.

Keywords

References

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