Journal of Acupuncture Research
- Volume 22 Issue 3
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- Pages.215-225
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
Effect of Carthami Tinctorii Fructus Herbal-acupuncture Solution(CTF-HAS) on Gene Expression in HepG2 carcinomar cells
Oligonucleotide chip를 이용한 홍화자약침액(紅花子藥鍼液)이 간암세포주(肝癌細胞柱)의 유전자(遺傳子) 발현(發顯)에 미치는 영향(影響)
- Lee, Kyung-min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Lim, Seong-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Jung, Tae-young (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University) ;
- Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Daegu Haany University)
- 이경민 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 정태영 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 서정철 (대구한의대학교 한의과대학 침구경혈학교실) ;
- 한상원 (대구한의대학교 한의과대학 침구경혈학교실)
- Received : 2005.05.09
- Accepted : 2005.05.14
- Published : 2005.06.20
Abstract
Objective : It has long been known about the osteogenic effect of CTF-HAS on bone tissues. However, it has not been determined the effect of CTF-HAS on cancer cells. The purpose of this study is to screen the CTF-HAS mediated differentially expressed genes in cancer cells such as HepG2 hepatoma cells lines. Oligonucleotide microarray approach were employed to screen the differential expression genes. Methods : CTF-HAS was prepared by boiling and stored at
홍화자약침액(紅花子藥鍼液)의 항암효능(抗癌效能)을 밝히고자 간암세포주(肝癌細胞柱)에 최신 oligonucleotide chip assay 법을 통하여 대양(大量)의 유전자(遺傳子) 발현(發顯)을 분석(分析)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1. MTT 분석(分析)에서 간암(肝癌) 및 위암세포주(胃癌細胞柱)는 홍화자약침액(紅花子藥鍼液) 1.5, 10, 20m/