Journal of Acupuncture Research
- Volume 22 Issue 3
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- Pages.185-200
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- 2005
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- 2586-288X(pISSN)
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- 2586-2898(eISSN)
The Experimental Study on the Immuno-regulatory effect of ASARI HERBA CUM RADICE Herbal- acupuncture at Pyesu(BL13) on OVA-induced asthma in mice
폐유(肺兪) 세신약침(細辛藥鍼)이 OVA-induced Asthma Mouse Model의 면역조절작용(免疫調節作用)에 미치는 영향
- Ryu, Eun-sang (Dept. of Acupuncture & Moxibustin, College of Oriental Medicine, Daejeon University) ;
- Lee, Hyun (Dept. of Acupuncture & Moxibustin, College of Oriental Medicine, Daejeon University) ;
- Lee, Byung-ryul (Dept. of Acupuncture & Moxibustin, College of Oriental Medicine, Daejeon University)
- Received : 2005.04.30
- Accepted : 2005.05.14
- Published : 2005.06.20
Abstract
Objective : The aim of this study was to investigate the asthma-suppressive and immune-regulatory effect of AHCR-HA(ASARI HERBA CUM RADICE Herbal-acupuncture) at Pyesu(BLl3) on OVA(ovalbumin)-induced asthma in mice. Methods : C57BL/6 mice out of all the experimental groups, except the Normal group and the AHCR-HA group, were sensitized and challenged with OVA The mice in the AHCR-HA group and the OVA-AHCR-HA group were treated with AHCR-HA(1%) at Pyesu(BL13). The mice in the OVA-Saline group were injected with saline at Pyesu(BL13). The mice in the OVA-Needle-Prick group were treated with a single prick with an injection needle at Pyesu(BL13). AHCR-HA saline injection and needle prick were administered for 8 weeks, three times a week. Result : 1. The populations of granulocytes, CD3e-/CCR3+ cells, CD69+/CD3e+ cells, CD4+ cells and CD23+/B220+ cells in the OVA-induced asthmatic mouse lungs decreased significantly by AHCR-HA. 2. The lung weight, total cells in lung, total leukocytes in BALF, eosinophils in BALF, collagen accumulation in the lung sections of the OVA-AHCR-HA group decreased significantly. 3.The concentrations of IL-4, IL-5, IL-13, IgE in BALF and serum of the OVA-AHCR-HA group decreased significantly. 4. The numbers of Gr-1+/CD11b+, CCR3+, CD3e+, CD19+, CD3e+/CD69+cells in the OVA-AHCR-HA group decreased significantly. 5. The mRAN expressions of
폐유(肺兪) 세신약침(細辛藥鍼)(AMCR-HA)이 정상 mouse와 알레르기 喘息이 유발된 mouse의 면역기전(免疫機轉)에 미치는 영향(影響)을 비교검토(比較檢討)하기 위하여 C57BL/6 mouse에 알레르기 천식병태(喘息病態)를 유발(誘發)하고 천식(喘息)이 유발(誘發)된 mouse와 정상 mouse의 폐유(肺兪) (BLl3)에 세신약침(細辛藥鍼)을 시술(施術)한 후, in vitro 및 in vivo 실험을 통해 폐유(肺兪) 세신약침(細辛藥鍼)이 천식억제(喘息抑制) 및 면역조절작용(免疫調節作用)에 미치는 영향(影響)을 관찰(觀察)하여 다음과 같은 결론(結論)을 얻었다. in vitro 1. 폐내(肺內) 호립구(灝粒球),