Antibacterial Activity In Vitro and Primary Dermal Irritation Test in Rabbits of Surfactin Produced Bacillus subtilus Complex BC2121

Bacillus subtilus Compulex BC1212에서 생산된 Surfactin의 시험관내 항균활성 및 토끼에서 일차피부자극시험

  • Hwang Mi-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Yun Hyo-In (College of Veterinary Medicine, Chungnam National University) ;
  • Lim Jong-Hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Kim Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee Man-hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Park Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2005.03.01

Abstract

The present study was carried out to investigate the antibacterial activity against Staphylococcus aureus with antibiotic-resistance in vitro and the skin irritation in rabbits with surfactin produced by Bacillus subtilus Complex BC1212. The antibacterial activities of selected antimicrobial agents (surfactin, amoxacillin, colistin, norfloxacin and streptomycin) were evaluated by using the broth microdilution method. As the results, the minimum inhibitory concentration (MIC) of the surfactin was less than 15.6 ㎍/ml. In the skin irritation test, two out of 4 rabbits showed very slight edema at 24 h after the administration of surfactin, and then recovered at 72 h. The change of body weight was normal during the skin irritation test. The primary irritation index in accordance with the Draize evaluation of topical reaction was calculated to be '0.125', which meant not irritating. Based on these results, it could be concluded that the test agent, surfactin, was a non-irritant. We could also think that the surfactin may be useful for the treatment of S. aureus infections such as bovine mastitis.

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References

  1. Arima, K., Kakinuma, A. and Tamura, G. (1968): Surfactin, a crystalline peptidelipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation. Biochem. Biophys. Res. Commun., 31, 488-494 https://doi.org/10.1016/0006-291X(68)90503-2
  2. Draize, J.H., Woodard, G. and Calvery, H.O. (1944): Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J. Pharmacol. Exp. Ther., 82, 377-390
  3. Geornaras, I. and von Holy, A. (2001): Antimicrobial susceptibilities of isolates of Staphylococcus aureus, Listeria species and Salmonella serotypes associated with poultry processing. Int. J. Food Microbiol., 70, 29-35 https://doi.org/10.1016/S0168-1605(01)00517-7
  4. Imai, Y., Sugino, H. and Takinuma, A. (1971): Hypocholesterolemic effect of surfactin, a novel bacterial peptide lipid. Takeda Kenkyusho Ho., 30, 728-734
  5. Kakinuma, A., Hori, M., lsono, M., Tamura, G. and Arima, K. (1969): Determination of amino acid sequence in surfactin, a crystalline peptidolipid surfactant produced by Bacillus subtilis. Agric. Biol. Chem., 33, 971-972 https://doi.org/10.1080/00021369.1969.10859408
  6. Kameda, Y., Matsui, K., Kato, H., Yamada, T. and Sagai H. (1972): Antitumor activity of Bacillus natto. 3. Isolation and characterization of a cytolytic substance on Ehrlich ascites carcinoma cells in the culture medium of Bacillus natto KMD 1126. Chem. Pharm. Bull., 20, 1551-1557 https://doi.org/10.1248/cpb.20.1551
  7. Kim, K., Jung, S.Y., Lee, D.K., Jung, J.K., Park, J.K., Kim, D.K. and Lee, C.H. (1998); Suppression of inflammatory responses by surfactin, a selective inhibitor of platelet cytosolic phospholipase A2. Biochem. Pharmacol., 55, 975-985 https://doi.org/10.1016/S0006-2952(97)00613-8
  8. Tsukagoshi, N., Tamura, G. and Arima, K. (1970): A novel protoplast-bursting factor (surtactin) obtained from Bacillus subtilis lAM 1213. II. The interaction of surtactin with bacterial membranes and lipids. Biochim. Biophys. Acta., 196, 211-214 https://doi.org/10.1016/0005-2736(70)90008-8