Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Antibacterial Activity In Vitro and Primary Dermal Irritation Test in Rabbits of Surfactin Produced Bacillus subtilus Complex BC2121

Bacillus subtilus Compulex BC1212에서 생산된 Surfactin의 시험관내 항균활성 및 토끼에서 일차피부자극시험

  • Hwang Mi-Hyun (College of Veterinary Medicine, Kyungpook National University) ;
  • Yun Hyo-In (College of Veterinary Medicine, Chungnam National University) ;
  • Lim Jong-Hwan (College of Veterinary Medicine, Chungnam National University) ;
  • Kim Kil-Soo (College of Veterinary Medicine, Kyungpook National University) ;
  • Rhee Man-hee (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim Nam-Woo (Department of Herbal Biotechnology, Daegu Haany University) ;
  • Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Park Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
  • Published : 2005.03.01
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Abstract

The present study was carried out to investigate the antibacterial activity against Staphylococcus aureus with antibiotic-resistance in vitro and the skin irritation in rabbits with surfactin produced by Bacillus subtilus Complex BC1212. The antibacterial activities of selected antimicrobial agents (surfactin, amoxacillin, colistin, norfloxacin and streptomycin) were evaluated by using the broth microdilution method. As the results, the minimum inhibitory concentration (MIC) of the surfactin was less than 15.6 ㎍/ml. In the skin irritation test, two out of 4 rabbits showed very slight edema at 24 h after the administration of surfactin, and then recovered at 72 h. The change of body weight was normal during the skin irritation test. The primary irritation index in accordance with the Draize evaluation of topical reaction was calculated to be '0.125', which meant not irritating. Based on these results, it could be concluded that the test agent, surfactin, was a non-irritant. We could also think that the surfactin may be useful for the treatment of S. aureus infections such as bovine mastitis.

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References

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