The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Effects of Alternative Medicine Extract on Bone Mineral Density, Bone Strength and Biochemical Markers of Bone Metabolism in Ovariectomized Rats

난소적출 수술을 받은 흰쥐에서의 골밀도, 골강도 및 골대사의 생화학적 지표에 대한 한방요법의 효과

  • Lee Joo-Won (Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine, Hanyang University) ;
  • Kim Hyunjin (Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine, Hanyang University) ;
  • Jhee Okhwa (Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine, Hanyang University) ;
  • Won Haedan (Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine, Hanyang University) ;
  • Yu Youngjo (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Lee Minho (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Kim Taewha (Department of Internal Medicine, College of Medicine, Hanyang University) ;
  • Om Aeson (Department of Food Science and Nutrition, College of Human Ecology, Hanyang University) ;
  • Kang Juseop (Department of Pharmacology & Clinical Pharmacology Laboratory, College of Medicine, Hanyang University)
  • 이주원 (한양대학교 의과대학 약리학실) ;
  • 김현진 (한양대학교 의과대학 약리학실) ;
  • 지옥화 (한양대학교 의과대학 약리학실) ;
  • 원해단 (한양대학교 의과대학 약리학실) ;
  • 유영조 (한양대학교 의과대학 약학과) ;
  • 이민호 (한양대학교 의과대학 약학과) ;
  • 김태화 (한양대학교 의과대학 약학과) ;
  • 엄애선 (한양대학교 생활과학대학 식품영양학과) ;
  • 강주섭 (한양대학교 의과대학 약리학실)
  • Published : 2005.03.01
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Abstract

To prevent and treat the osteoporosis, diverse therapies have been applied, which is still need to solve negative side effects. We investigated the effects of the extract from alternative medicine(AM) on the progress of bone loss in ovariectomized-rats fed with Ca-deficient diet for 7 or 14 weeks. Ovariectomy(OVX) concomitant with Ca-deficiency caused bone loss evidently decreased in bone mineral density and bone strength of femoral epiphysis and vertebrae, which were ameliorated with administration of AM extract. Also, the effect of AM extract on the biochemical markers were measured. The increased serum alkaline phosphatase caused by OVX and Ca-deficiency were observed, which were not affected by administration of AM extract. Administration of AM extract may have preventive effect on the elevated serum acid phosphatase concentrations caused by OVX and Ca-deficiency at 14 wks, implicating that AM extract possibly acts toward reducing born resorption, even though the results were not statistically significant. Serum osteocalcin and urinary deoxypyridinoline, the markers of bone turn over, were not changed by estrogen deficiency or AM extract. We concluded that the AM extract treatment had potently preventive effects on the decreased bone density and bone strength induced by OVX and Ca-deficiency. The changes of biochemical markers related to the effect of AM extract were not manifested but it still suggest that AM extract may inhibit the bone resorption derived from OVX and Ca-deficiency.

본 실험은 칼슘 결핍과 난소 제거로 유발한 골다공증에 대한 한방요법의 효과에 대하여 고찰하였다. 1) 난소 제거로 인한 에스트로겐의 결핍 및 칼슘 결핍은 폐경여성에서 관찰되어지는 것처럼 골강도 및 골밀도의 손실을 일으켰다. 2) 한방 추출물의 투여는 에스트로겐 결핍 및 칼슘 결핍으로 유도된 골강도 및 골밀도 손실에 대한 예방효과가 관찰되었다 3) 난소제거로 인한 에스트로겐의 결핍 및 칼슘 결핍은 골대사의 생화학적 지표들(혈청 중 alkaline phosphatase, acid phosphatase, osteocalcin, 그리고 뇨중 deoxyopyridinoline 농도)에 영향을 미치지 않았다. 4) 한방 추출물의 투여 또한 골 대사의 생화학적 지표들에 영향을 미치지 않았다. 결론적으로, 한방 추출물의 골손실에 대한 예방효과는 한방 추출물의 골다공증 치료제로서의 가능성을 제시하며, 새로운 골다공증 치료제 개발 일환으로 한방추출물의 적용기간 및 농도 변화를 통한 계속적인 연구가 요구되어진다.

Keywords

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