The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Studies on Antioxidative Effect and Lactic Acid Bacteria Growth of Persimmon Leaf Extracts

감잎 추출물의 유산균 생육 및 항산화 효과에 관한 연구

  • Woo Jun-Young (Dept. of Food Science & Biotechnology, Food Science & Biotechnology Research Center, Hankyong National University) ;
  • Paek Nam-Soo (Mediogen, Co. Ltd) ;
  • Kim Young-Man (Dept. of Food Science & Biotechnology, Food Science & Biotechnology Research Center, Hankyong National University)
  • 우준영 (한경대학교 식품생물공학과 및 식품생물 산업연구소) ;
  • 백남수 ((주)메디오젠) ;
  • 김영만 (한경대학교 식품생물공학과 및 식품생물 산업연구소)
  • Published : 2005.03.01
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Abstract

This study was investigated the growth effect of persimmon leaf extracts on various Lactic acid bacteria, the effect as freeze-dry cryoprotectan, by the changes of vitamin C content, electron donating ability, total cell count and SOD-like activity. The total cell count of L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530 and L. casei MG311 in the absence of persimmon leaf extracts(10%) at 37℃ after 48hr were 3.2×10/sup 9/cfu/mL, 2.9×10/sup 9/cfu/mL, 1.1×10/sup 9/cfu/mL, 1.6×10/sup 9/cfu/mL and 3×10/sup 9/cfu/mL, respectively, and pH and total acidity of those were 3.82∼3.88, and 1.697∼1.842. On the other hand, the total cell count of L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530 and L. casei MG311 in the presence of persimmon leaf extracts at 37℃ after 48hr were 4.4×10/sup 9/cfu/mL, 4.3×10/sup 9/cfu/mL, 2×10/sup 9/cfu/mL, 3.3×10/sup 9/cfu/mL, and 3.4×10/sup 9/cfu/mL, respectively, and pH and total acidity of those were 3.74∼3.82 and 1.528∼1.805. The total cell counter of lactic acid bacteria in the presence of persimmon leaf extracts(10%) at 37℃ after 48hr higher than those in the absence of persimmon leaf extracts. In freeze-dry chryoprotectan, the survival rate of L. acidphilus MG501 decreased about 30%. In case of L. brevis MG19 and L. bulgaricus MG515, the survival rate decreased about 10%. However, the survival rate of L. lactis MG530 increased about 10% and L. casei MG311 showed the almost same effect. The changes of vitamin C content were the highest in L. lactis MG530(190.26mg/mL) and the lowest in L. acidophilus MG501(56.05 mg/mL). The electron donating ability indicated to L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530 and L. casei MG311 added by 10% persimmon leaf extracts, respectively. when 10% persimmon leaf extracts were added L. brevis MG19, the electron donating ability is the highest of all lactic acid bacteria. The SOD-like activity of L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530 and L. casei MG311 showed lower than that of control.

본 연구는 감잎 추출물을 이용하여 L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530, L. casei MG311 균종에 대한 생육조건 및 동결건조 보호제로서의 효과, 가혹조건에서의 경시변화, vitamin C 함량 변화, DPPH 라디칼 소거효과, SOD 유사활성을 조사하였다. 유산균 생육은 L. acidophilus MG501, L. brevis MG19, L. bulgaricus MG515, L. lactis MG530, L. casei MG311에서 각각 37℃/48hr 동안 배양한 후 3.2×10/sup 9/cfu/mL, 2.9×10/sup 9/cfu/mL, 1.1×10/sup 9/cfu/mL, 1.6×10/sup 9/cfu/mL, 3×10/sup 9/cfu/mL 생균수을 나타냈으며 pH는 3.82∼3.88, 산도는 1.697∼l.842를 나타내었다. 반면에 감잎추출물 10% 첨가에 4.4×10/sup 9/cfu/mL, 4.3×10/sup 9/cfu/mL, 2×10/sup 9/cfu/mL, 3.3×10/sup 9/cfu/mL, 3.4×10/sup 9/cfu/mL에 생균수을 보여 유산균 생육시 감잎 추출물 첨가가 유산균 증식에 효과를 보였으며 pH는 3.74∼3.82, 산도는 1.528∼1.805로 낮게 나타내었다. 동결건조 보호제에서는 L. acidophilus MG501는 약30%정도 생존율의 감소를 보였으며 L. brevis MG19, L. bulgaricus MG515의 경우는 약 10% 정도 생존율 감소를 보였다. 반면에 L. lactis MG530는 약 10%정도 생존율 증가를 보였으며 L. casei MG311은 거의 동등한 효과를 나타냈다. Vitamin C 함량 변화는 L. lactis MG530에서 190.26mg/mL로 가장 높게 나타났으며 L. acidophilus MG501에서는 56.05mg/mL로 가장 낮게 나타났다. DPPH 라디칼 소거효과는 감잎 추출물 10% 첨가시에 높았으며 그 중에서도 L. brevis MG19에 감잎 추출물을 10% 첨가하여 배양한 경우 효과가 가장 높게 나타났다. SOD 유사활성은 전반적으로 대조군보다 비교적 낮은 활성을 나타냈다.

Keywords

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