Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Effects of Polycyclic Aromatic Hydrocarbons (PAHs) on Early Development of Sea Urchin Anthocidaris crassispina

Polycyclic aromatic hydrocarbons (PAHs)가 보라성게 (Anthocidaris crassispina)의 초기발생에 미치는 영향

  • Na Oh-Soo (Marine and Environmental Research Institute, Cheju National University) ;
  • Moon Sang-Hee (Division of Civil and Environmental Engineering, Cheju National University) ;
  • Lee Chi-Hoon (Marine and Environmental Research Institute, Cheju National University) ;
  • Park Chang-Beom (Marine and Environmental Research Institute, Cheju National University) ;
  • Kim Byung-Ho (Jeju Hi-Tech Industry Development Institute) ;
  • Jin Young-Seok (Marine and Environmental Research Institute, Cheju National University) ;
  • Hur Sang-Woo (Marine and Environmental Research Institute, Cheju National University) ;
  • Kam Sang-Kyu (Division of Civil and Environmental Engineering, Cheju National University) ;
  • Lee Young-Don (Marine and Environmental Research Institute, Cheju National University)
  • 나오수 (제주대학교 해양과환경연구소) ;
  • 문상희 (제주대학교 토목환경공학전공) ;
  • 이치훈 (제주대학교 해양과환경연구소) ;
  • 박창범 (제주대학교 해양과환경연구소) ;
  • 김병호 ((재)제주하이테크산업진흥원) ;
  • 진영석 (제주대학교 해양과환경연구소) ;
  • 허상우 (제주대학교 해양과환경연구소) ;
  • 감상규 (제주대학교 토목환경공학전공) ;
  • 이영돈 (제주대학교 해양과환경연구소)
  • Published : 2005.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Effects of five polycyclic aromatic hydrocarbon (PAHs) constituents (naphthalene, fluorine, fluoranthene, benzo(a)pyrene, pyrene) on fertilization and early development of sea urchin egg, sperm and fertilized egg were investigated. The eggs, sperm and fertilized eggs were exposed to several concentrations of PAHs (1, 10, 100, 1000 and 10000㎍/L). The rate of fertilization and hatching decreased when the eggs and sperm were exposed to aqueous solution of PAHs. Also, Exposure of fertilized eggs with each PAHs did decrease survival and hatching rate. Concentration-dependent toxic effects on the rate of fertilization, hatching, survival and abnormality in A. crassispina were observed following exposure to PAHs (1, 10, 100, 1000 and 10000㎍/L). These data show that PAHs exposure decreased in fertilization success of sea urchin egg and sperm and producted abnormal embryo. It is plausible to suggest that PAHs had the potential to significantly reduce coastal recruitment of sea urchin.

Keywords

References

  1. Au DWT, Lee CY, Chan KL and Wu RSS. Reproductive impairment of sea urchins upon Chronic exposure to cadmium. Part I: Effects on gamete quality, Environmental Pollution 2001; 111: 1-9 https://doi.org/10.1016/S0269-7491(00)00035-X
  2. Barron MG, Podrabsky T, Ogle S and Richer RW. Are aromatic hydrocarbons the primary determinant of petroleum toxicity to aquatic organisms?, Aquatic Toxicology 1999; 46: 253-268 https://doi.org/10.1016/S0166-445X(98)00127-1
  3. Bartlett JHG, Mageean DM and O'Conner RJ. Residential expansion as a continental threat to U.S. coastal ecosystem. Popul Environ 2000; 21: 429-468 https://doi.org/10.1007/BF02436749
  4. Earnshow MJ, Wilson S, Akberali HB, Butler RD and Marriott KRM. Theaction of heavy metals on the gametes of the marine mussel, Mytilus edulis (L.) III. The effect of applied copper and zinc on sperm motility in relation to ultrastructural damage and intercellular metal localization, Marine Environmental Research 1986; 20(4): 261-278 https://doi.org/10.1016/0141-1136(86)90052-8
  5. Fent K and atscher BR. Cytochrome P450A induction potencies of polycyclic aromatic hydrocabons in a fish hepatoma cell line: demonstation of additive interactions and an induction equivalency concept, Environ Toxicol Chem 2000; 19: 2047-2058 https://doi.org/10.1897/1551-5028(2000)019<2047:CPIPOP>2.3.CO;2
  6. Giessing AM, Mayer LM and Forbes TL. Synchronous fluorescence spectrometry of 1-hydroxypyrene: a rapid screening method for identification of PAH exposure in tissue from marine polychaetes, Mar Environ Rea 2003; 56(5): 599-615 https://doi.org/10.1016/S0141-1136(03)00045-X
  7. Ghoshal S, Weber WJ, Rumel AM, Trosko JE and Upham BL. Epigenetic toxicity of a mixture of polycyclic aromatic hydrocarbons on gap junctional intercellular communication before and after biodegradation, Envrion Sci Technol 1999; 33: 1044-1050 https://doi.org/10.1021/es9809511
  8. Green JD, Mwatibo JM and Swartz WJ. The effects of methoxychlor on early sea urchin development, Envrionmental reacherch 1997; 72: 56-64 https://doi.org/10.1006/enrs.1996.3692
  9. Jackim E and Nacci D. Improved sea urchin DNA-based embryo growth toxicity test, Environmental Toxicology and Chemistry 1986; 5: 561-565 https://doi.org/10.1897/1552-8618(1986)5[561:ISUDEG]2.0.CO;2
  10. Heit M, Tan YL and Miller KM. The origin and deposition history of polycyclic aromatic hydrocaborns in the finger lakes region of New York USA, Water Air Soil Pollut 1988; 37: 85-110 https://doi.org/10.1007/BF00226482
  11. Incardona JP, Collier TK and Scholz NL. Defects in cardiac function precede morphological abnormalities in fish embryos exposed to polycyclic aromatic hydrocarbons, Toxicol Applied Pharmacol 2004; 196: 191-205 https://doi.org/10.1016/j.taap.2003.11.026
  12. Kanous KS, Casey C and Lindemann CB. Inhibition of microtubule sliding by nickel and cadmium: evidence for a differential response of certain microtublule pairs within the bovine sperm axoneme, Cell Motility and Cytoskeleton 1993; 26: 66-76 https://doi.org/10.1002/cm.970260107
  13. Khan AT and Weis JS. Differential effects of organic and inorganic mercury on the micropyle of the eggs of Fundulus heteroclitus, Environ Bio Fish 1993; 37: 323-327 https://doi.org/10.1007/BF00004640
  14. Kobayashi N. Marine pollution bioassay by using sea urchin eggs in the Tanabe Bay, Wakayama Prefecture, Japan, 1970-1987, Mar Pollut Bull 1991; 23: 709-713 https://doi.org/10.1016/0025-326X(91)90765-K
  15. Marano J, Krishn FS, Wamy S, Betrencourt C, Schoevaert D, Provost JM and Volochine B. Cnotrol of ciliary beat by calcium: the effects of lindane, a potent insecticide, Biol Cell 1988; 63: 143-150 https://doi.org/10.1016/0248-4900(88)90053-6
  16. McElroy AE, Bates S, Rice SD and Korn S. Bioavailability of polycyclic aromatic hydrocarbons in the aquatic environment. In: Varanasi, U. (Ed.), Metabolism of polycyclic aromatic hydrocarbons in the aquatic environment, CRC Press. Boca Raton FL 1985. pp. l- 39
  17. Meteyer MJ, Wright DA and Martin FD. Effects of cadium on early developmental stages of the sheepshead minnow (Cyprinodon variegatus), Environ Toxicol Chem 1988; 7: 321-328 https://doi.org/10.1897/1552-8618(1988)7[321:EOCOED]2.0.CO;2
  18. Mwatibo JM and Green JD. Effects of methoxychlor preexposure on sea urchin gametes, Bull Envrion Contam Toxicol 1997; 58: 589-595 https://doi.org/10.1007/s001289900375
  19. Mwatibo JM and Green JD. Appearnce of new glycoprotein in methoxochlor-exposed sea urchin gastrulae, Bull Envrion Contam Toxicol 1998; 60: 791-796 https://doi.org/10.1007/s001289900696
  20. National Research Council. Oil in the Sea III: Inputs, Fates, and Effects. National Academics Press. Washington. DC. 2003. p. 446
  21. Ozretic B, Petrovic S and Krajnovic-Ozretic M. Toxicity of TBT-based paint leachates on the embryonic development of the sea urchin Paracentrotus lividus. Chemoshere 1998; 37(6): 1109-1118 https://doi.org/10.1016/S0045-6535(98)00109-X
  22. Pagano G, Corsale G, Esposito A, Dinnel PA and Romana LA. Use of sea urchin sperm and embryo bioassay in testing the sublethal toxicity of realistic pollutant level, Adv Appl Biotech Ser 1989; 5: 153-163
  23. Pesando D, Robert S, Huitorel R, Gutknecht E, Pereira L, Girard JP and Ciapa B. Effects of methoxychlor, dieldrin and lindane on sea urchin fertilization and early development, Aquatic Toxicology 2004; 66: 225-239 https://doi.org/10.1016/j.aquatox.2003.09.007
  24. Pillai MC, Vines CA, Wikramanayake AH and Cherr GN. Polycyclic aromatic hydrocarbons disrupt axial development in sea urchin embryo through a ${\beta}$-catenin dependent pathway, Toxicology 2003; 186: 93-108 https://doi.org/10.1016/S0300-483X(02)00695-9
  25. Roepke TA, Snyder MJ and Cherr GN. Estradiol and endocrine disrupting compounds adversely affect development of sea urchin embryos at environmentally relevant concentrations, Aqua Toxicol 2005; 71: 155-173 https://doi.org/10.1016/j.aquatox.2004.11.003
  26. Roseng L, Rivedal E, Skaare and Sanner T. Effect of 1,1'-(2,2,2-trichloroethyli- dene)-bis (4-chlorbenzene) (DDT) on gap junctional intercellular communication and morphological transformation of Syrina hamster embryo cells, Chem Biol Interact 1994; 90: 73-85 https://doi.org/10.1016/0009-2797(94)90112-0
  27. Silvestroni L, Fiorini R and Palleschi S. Partition of the organochlorine insecticide lindane into the human sperm surface induces membrane depolarization and $Ca^{2+}$ influx, Biochem J 1997; 321: 691-698 https://doi.org/10.1042/bj3210691
  28. Singh J, Viswanathan PN, Gupta P and Devi S. Changes in uptake of calcium caused by phytotoxicity of cadmium in Salvinia molesta, Ecotoxicology 1996; 5: 9-21 https://doi.org/10.1007/BF00116320
  29. Swann K and Whitaker MT. Second messengers at fertilization in sea-urchin eggs, Journal of Reproduction and Fertilization 1990; 42: 141-154
  30. Tkahashi, K and Kamimura S. Dynamic aspects of microtubule sliding in sperm flagella, Journal of Submicroscope Cytology 1983; 15: 1-3
  31. Van Metre PC, Barbara J Mahler and Edward T Furlong. Urban sprawl leaves its PAH signature, Environmental Science & Technology 2000; 34(19): 4064-4070 https://doi.org/10.1021/es991007n
  32. Varanasi U. Metabolism of polycyclic aromatic hydrocabons in the aquatic environment, CRC. Boca Raton FL USA. 1989
  33. Venturini N and Tommasi LR. Polycyclic aromatic hydrocarbons and changes in the trophic structure of polychaete assemblages in sediements of Todos os Santos Bay, Northeastern, Brazil Mar Poll Bull 2004; 48(1-2): 97-107 https://doi.org/10.1016/S0025-326X(03)00331-X
  34. Wilson DP. A biological difference between natural waters, J Mar Biol Assoc UK 1951; 30: 1-21 https://doi.org/10.1017/S0025315400012522