ALGAE

  • 제20권1호
  • /
  • Pages.61-67
  • /
  • 2005
  • /
  • 1226-2617(pISSN)
  • /
  • 2093-0860(eISSN)
한국조류학회(藻類) (The Korean Society of Phycology)
권호 표지
DOI QR코드

DOI QR Code

Characterization of Agarose Product from Agar Using DMSO

  • Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University) ;
  • Athukorala, Yasantha (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Je-Hee (Faculty of Applied Marine Science, Cheju National University)
  • 발행 : 2005.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Agar was extracted from Gelidium amansii, which was harvested at the shores of Jeju Island in South Korea. As a unique solvent, the ability of dimethyl sulfoxide (DMSO) was used to separate agarose from agar by removing agaropectine and quality of the resultant agarose was characterized for chromatography purposes. Agar sample was agitated by motor-driven stirrer with DMSO in a water bath (at 70$^{\circ}C$ for 2 h) and centrifuged (3,000 rpm for 20 min). Resultant upper agarose layer was gelled, washed, dried and milled. The quality of agarose was evaluated by the analysis of proximate chemical composition, sulfate content, gelling strength and DNA migration. In this study, the separated agarose showed low sulfate amount (0.28%) and showed high gel strength (1190 g ${\cdot}\;cm^{-2}$). The resolution power and the ligase activities gave clear picture about the suitability of the present agarose for practical purposes.

키워드

참고문헌

  1. 김미경, 2005. 생물활성수를 함유한 배지조성물 및 그 제조방법. 특 허등록번호 제05003332호
  2. 김미경, 김영환. 1999. 일반조류학. 도서출판 아카데미서적 229 pp
  3. 김세권, 백호철, 변희국, 강옥주, 김종해. 2001. 해양미세조류의 생 화학적 조성 및 항산화성. 한국수산학회지 34: 260-267
  4. 박미경, 이석준, 서현효, 김희식, 김영호, 윤병대, 오희목. 1998. Scenedesmus quadricauda를 이용한 축산폐수의 고도처리. 한국 조류학회지 13: 227-233
  5. 안주희, 김성수, 김태호, 이준엽, 오상집, 이진하, 이현용. 1996. 축 산폐수의 효율적 처리를 위한 광합성 미세조류인 Spirulina platensis 배양 공정의 최적화. 산업미생물학회지 24: 519-524
  6. 오희목, 이석준. 1998. 미세조류를 이용한 생물공학산업의 현황과 전망. 한국해양미세조류은행의 현황과 전망, 한국해양미세조 류은행 부경대학교 수산과학연구소 Proceedings 49-65
  7. 이인규, 부성민, 이상희. 2004. 조류의 다양성과 계통. 라이프 사이 언스 262 pp
  8. 임진영, 조만기, 한봉호. 1998. 해수산 Chlorella의 최적 배양 조건에 관한 연구. 한수지 31: 139-142
  9. 환경부. 2001. 공정시험방법. 669 pp
  10. APPA, AWWA. WEF. 1995. Standard methods for the examination of water and wastewater. In: Eaton A.D., Clesceri L.S. and Greenberg A.E. (eds), Baltimore 10: 157 pp
  11. Gilberst E. and Hoffmann-Glewe S. 1991. Ozonation of ethylenediaminetetraacetic acid (EDTA) in aqueous s olution influence of pH value and metal ions. Water Research 24: 39-44 https://doi.org/10.1016/0043-1354(90)90062-B
  12. Guillard R.R.L. 1975. Division rates. In: Stein J.R. (ed), Handbook of Phycological Methods-Culture Methods and Growth Measurements. Cambridge University Press, Cambridge. pp 289-311
  13. Holm-Hansen O., Lorenzen C.J., Holmes R.W., and Strickland J.D.H. 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer. 30: 3-15 https://doi.org/10.1093/icesjms/30.1.3
  14. Jeffrey S.W. 1972. Preparation and son properties of crystalline chlorophyll $c_{1}$ and $c_{2}$ from marine algae. Biochim. Biophys. Acta., 279: 15-33 https://doi.org/10.1016/0304-4165(72)90238-3
  15. John D.M., Whitton B.A. and Brook A.J. 2002. The Freshwater of Algal Flora of the British Isles: An Identification Guide to Freshwater and Terrestrial Algae. Cambridge Unicersity Press, Cambridge
  16. Kawamoto S. 1996. Experiment Results of BMW Techniques in the Farm of Kamegawa. Ecopeace Press, Daegu
  17. Kim M.K., Dubacq J.P., Thomas J.C. and Giraud G. 1996. Seasonal variation of triacylglycerols and fatty acids in Fucus serratus. Phytochemistry 43: 49-55 https://doi.org/10.1016/0031-9422(96)00243-9
  18. Kim M.K. and Giraud G. 1989. Characters of neutral lipids of Detonula sp. (Diatom) in culture. The Korean J. of Phycol. 4: 55-61
  19. Kim M.K., Park J.W., Park C.S., Jeune S.M., Kim S.J., Jeune K.H., Jang M.U. and Acreman J. 2006. Enhanced production of Scenedesmus spp. (green microalgae) using a new medium containing fermented swine wastewater. Bioresources Technol. (accepted)
  20. Kim M.K. and Smith R.E.H. 2001. Effect of ionic copper toxicity on the growth of green alga, Selenastrum capricornutum. J. Microbiol. Biotechnol. 11: 211-216
  21. Kim M.K. and Thomas J.C. 1991. Studies of growth according to the concentration of mineral elements of medium in Cyanophyte SG 63. Korean J. Bot. 35: 1-8
  22. Lee K. and C.G. Lee. 2001. Effect of light/dark cycles on wastewater treatment by microalgae. Biotechnol. Bioprocess Eng. 6: 194-199 https://doi.org/10.1007/BF02932550
  23. Nagasaki H. 1998. Bacteria Save the Earth Challenge of BMW Technology, Ecopeace Press, Daegu
  24. Parsons T.R. and Strickland J.D.H. 1963. Discussion of spectrophotometric determination of marine-plant pigment, with revised equation for ascertaining chlorophylls and carotenoids. J. Mar. Res. 21: 155-163
  25. Shon Y.H., Nam K.S. and Kim M.K. 2004. Cancer chemopreventive potential of Scenedesmus cultured in medium based on swine wastewater. J. Microbiol. Biotechnol. 14: 158-161
  26. Sung K.D., Lee J.S., Shin C.S. and Park S.C. 1998. Enhanced cell growth of Chlorella sp. KR-1 by the addition of iron and EDTA. J. Microbiol. Biotechnol. 8: 409-411
  27. Vonshak A. 1997. Microalgal Biotechnology; New Development in Production Facilities and Product. Abstracts of 2nd APMBC and 3rd APCAB. Phuket, Thailand. 21 pp
  28. Walz H.G. 1999. Phytoplankton Analyzer Phyto-PAM: System Components and Principles of Operation. Walz, Effeltrich

피인용 문헌

  1. Biodegradable polymer scaffolds vol.4, pp.47, 2016, https://doi.org/10.1039/C6TB02176J
  2. Optimization of yield and quality of agar polysaccharide isolated from the marine red macroalga Pyropia yezoensis vol.26, 2017, https://doi.org/10.1016/j.algal.2017.07.010
  3. Bioactive compounds in seaweed: functional food applications and legislation vol.23, pp.3, 2011, https://doi.org/10.1007/s10811-010-9632-5
  4. Preparation of Commercial Agarose from Jeju Seaweed, Gelidium amansii using DMSO Extraction and EDTA Washing vol.44, pp.6, 2011, https://doi.org/10.5657/KFAS.2011.0635
  5. Four novel Gelidium species (Gelidiales, Rhodophyta) discovered in Korea: G. coreanum, G. jejuensis, G. minimum and G. prostratum vol.51, pp.4, 2012, https://doi.org/10.2216/11-117.1
  6. Complete mitochondrial genome of the agarophyte red algaGelidium vagum(Gelidiales) vol.25, pp.4, 2014, https://doi.org/10.3109/19401736.2013.800499
  7. A NEW AGAROPHYTE SPECIES, GELIDIUM EUCORNEUM SP. NOV. (GELIDIALES, RHODOPHYTA), BASED ON MOLECULAR AND MORPHOLOGICAL DATA1 vol.47, pp.4, 2011, https://doi.org/10.1111/j.1529-8817.2011.01005.x
  8. Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins pp.1573-5176, 2019, https://doi.org/10.1007/s10811-019-1735-z