DOI QR코드

DOI QR Code

Preliminary Study on the Toxicity and Transfer of Heavy Metals and Tributyltin to Seagrass Zostera marina

잘피의 광합성에 대한 중금속 및 TBT의 독성 영향과 중금속 흡수에 대한 연구

  • Published : 2005.06.01

Abstract

Uptake kinetics of Cd and Zn by leaves and rhizome of the seagrass Zostera marina were examined in controlled laboratory radiotracer experiments. Subsequently, acute toxicity of Cd, Cu and TBT on photosynthetic quantum yield (ΔF/Fm’ of Z. marina were determined, and the differential sensitivities of rapid light curve (RLC) to those harmful substances were also compared. All measurements on photosynthetic activity were determined by chlorophyll a fluorescence method using pulse amplitude modulation (PAM). Metal uptake by Z. marina was saturated with increasing exposure time in leaves and rhizomes. Uptake of Zn by Z. marina was faster than that of Cd. Metal uptake rates in Z. marina decreased with the increase of dissolved metal concentrations and also with the increase of biomass. The adverse effect of TBT on effective quantum yield was stronger than other pollutants. Average acute toxicity on the RLC of the seagrass exposed to TBT and two heavy metals (Cd and Cu) was going to decrease as follows: TBT > Cd > Cu. Our preliminary results in this study suggested that Z. marina potentially can be used as a biomonitor of harmful substances contamination in coastal waters.

Keywords

References

  1. Baron M., Arellano J.B. and Gorge J.L. 1995. Copper and photosystem II: a controversial relationship. Physiol. Plant 94: 174-180 https://doi.org/10.1111/j.1399-3054.1995.tb00799.x
  2. Barreiro R., Real C. and Carballeira A. 1993. Heavy-metal accumulation by Fucus ceranoides in a small estuary in North-West Spain. Mar. Environ. Res. 36: 39-61 https://doi.org/10.1016/0141-1136(93)90088-H
  3. Batley G.E. 1987. Heavy metals speciation in water, sediments and biota from Lake Macquarie, New Souther Wales. Aust. J. Mar. Freshwat. Res. 38: 591-606 https://doi.org/10.1071/MF9870591
  4. Clark R.B. 2001. Marine Pollution. 5ed. Oxford University Press, New York
  5. Clijsters H. and Van-Assche F. 1985. Inhibition of photosynthesis by heavy metals. Photosyn. Res. 7: 31-40 https://doi.org/10.1007/BF00032920
  6. Clijsters H., Cuypers A. and Vangronsveld J. 1999. Physiological response to heavy metals in higher plants-defence against oxidative stress. Z. Naturforsch. C54: 730-734
  7. Cullen J.J. and Lesser M.P. 1991. Inhibition of photosynthesis by ultraviolet radiation as a function of dose and dosage rate: results for a marine diatom. Mar. Biol. 111: 183-190 https://doi.org/10.1007/BF01319699
  8. de Filippis L.F. and Pallaghy C.K. 1994. Heavy metals: sources and biological effects. In: Rai L.C., Caur J.P. and Soeder C.J. (eds), Algae and water pollution: Advances in Limnology Series, Vol. 42. Schweizerbart, Stuttgart, pp 32-77
  9. de Mora S.J., Stewart C. and Phillips D. 1995. Sources and rate of degradation of tri(n-butyl)tin in marine sediments near Auckland, New Zealand. Mar. Pollut. Bull. 30: 50-57 https://doi.org/10.1016/0025-326X(94)00178-C
  10. Duarte C.M. 2002. The future of seagrass meadows. Environ. Cons. 29: 192-206
  11. Evans S.M., Leksono T. and McKinnell P.D. 1995. Tributyltin pollution: a diminishing problem following legislation limiting the use of TBT-based anti-fouling paints. Mar. Pullut. Bull. 30: 14-21 https://doi.org/10.1016/0025-326X(94)00181-8
  12. Fabris G.J., Harris J.E. and Smith J.D. 1982. Uptake of cadmium by the seagrass Heterozostera tasmnica from Corio Bay and Wester Port, Victoria. Aust. J. Mar. Freshw. Res. 33: 829-836 https://doi.org/10.1071/MF9820829
  13. Fent K. 1996. Ecotoxicology of organotin compounds. Crit. Rev. Toxicol. 26: 1-117
  14. Goldberg E.D., Koide M., Hodge V., Flegal A.R. and Martin J. 1983. U.S. mussel watch: 1977-1978 results on trace metals and radionuclides. Estuar. Cont. Shelf Sci. 16: 69-93 https://doi.org/10.1016/0272-7714(83)90095-1
  15. Govindjee R. 1995. Sixty-three years since Kautsky: Chlorophyll a fluorescence. Austr. J. Plant Physiol. 22: 131-160 https://doi.org/10.1071/PP9950131
  16. Hall M.O., Durako M.J., Fourqurean J.W. and Zieman J.C. 1999. Decadal changes in seagrass distribution and abundance in Florida Bay. Estuaries 22: 445-459 https://doi.org/10.2307/1353210
  17. Haritonidis S. and Malea P. 1995. Seasonal and local variation of Cr, Ni, and Co concentrations in Ulva rigida C. Agardh and Enteromorpha linza (Linnaeus) from Thermaikos Gulf, Greece. Environ. Pollut. 89: 319-327 https://doi.org/10.1016/0269-7491(94)00070-T
  18. Haynes D. and Johnson J.E. 2000. Organochlorine, heavy metal and polyaromatic hydrocarbon pollutant concentrations in the Great Barrier Reef (Australia) environment: a review. Mar. Pollut. Bull. 41: 267-278 https://doi.org/10.1016/S0025-326X(00)00134-X
  19. Hemminga M.A. and Duarte C.M. 2000. Seagrass ecology. Cambridge University Press, Cambridge
  20. Hugget R.J., Seligman P.F. and Valkiers A.O. 1992. The marine biocide tributyltin. Assessing and managing the environmental risks. Environ. Sci. Technol. 26: 232-237 https://doi.org/10.1021/es00026a001
  21. Jensen H.F., Holmer M. and Dahllof I. 2004. Effects of tributyltin (TBT) on the seagrass Ruppia maritima. Mar. Pollut. Bull. 49: 564-573 https://doi.org/10.1016/j.marpolbul.2004.03.010
  22. Jones A.L. and Harwood J.L. 1993. Lipid metabolism in the marine brown algae Fucus vesiculosus and Ascophyllum nodosum. J. Exp. Bot. 44: 1203-1210 https://doi.org/10.1093/jxb/44.7.1203
  23. Kang S.G., David A.W. and Koh C.H. 2000. Baseline metal concentration in the Asian periwinkle Littorina brevicula employed as a biomonitor to assess metal pollution in Korean coastal waters. Sci. Total Environ. 263: 143-153 https://doi.org/10.1016/S0048-9697(00)00695-1
  24. Kennish M.J. 1992. Practical handbook of estuarine and marine pollution. CRC Press, Boca Raton pp 524
  25. Kimimura M. and Katoh S. 1972. Studies on electron transport associated with photosystem I. I. Functional site of plastocyanin, inhibitory effects of $HgCl_{2}$ on electron transport and plastocyanin in chloroplast. Biochim. Biophys. Acta 283: 279-292 https://doi.org/10.1016/0005-2728(72)90244-7
  26. Larkum A.W.D. and West R.J. 1990. Long-term changes of seagrass meadows in Botany Bay, Australia. Aqua. Bot. 37: 55-70 https://doi.org/10.1016/0304-3770(90)90064-R
  27. Lee B.-G., Wallace W.G. and Luoma S.N. 1998. Uptake and loss kinetics of Cd, Cr and Zn in the bivalves Potamocorbula amurenssis and Macoma balthica: effects of size and salinity. Mar. Ecol. Prog. Ser. 175: 177-189 https://doi.org/10.3354/meps175177
  28. Lee K.W., Kang H.S. and Lee S.H. 1998. Trace elements in the Korean coastal Environment. Sci. Total Environ. 214: 11-19 https://doi.org/10.1016/S0048-9697(98)00051-5
  29. Lobban C.S. and Harrison P.J. 1997. Seaweed ecology and physiology. Cambridge Univ. Press, Cambridge
  30. Luna C.M., Gonzalez C.A. and Trippi V.S. 1994. Oxidative damage caused by an excess of copper in oat leaves. Plant Cell Physiol. 35: 11-15
  31. Lyngby E. and Brix H. 1982. Seasonal and environmental variation in cadmium, copper, lead and zinc concentrations in eelgrass (Zostera marina L.) in the Limfjiord, Denmark. Aquat. Bot. 14: 59-74 https://doi.org/10.1016/0304-3770(82)90086-9
  32. Lyngby J.E. and Brix H. 1984. The uptake of heavy metals in eelgrass Zostera marina and their effect on growth. Ecol. Bull. 36: 81-89
  33. Macinnis-Ng C.M.O. and Ralph P.J. 2002. Towards a more ecologically relevant assessment of the impact of heavy metals on the photosynthesis of the seagrass, Zostera capricorni. Mar. Pollut. Bull. 45: 100-106 https://doi.org/10.1016/S0025-326X(01)00300-9
  34. Malea P., Haritonidis S. and Kevrekidis T. 1995. The short-term uptake of copper by the two parts of the seagrass Halophila stipulacea (Forsk.) Ashers. and leaf-cells viability. Fresenius Environ. Bull. 4: 117-122
  35. Molander S., Dahl B., Blanck H., Jonsseon J. and Sj str m M. 1992. Combined effects of tri-n-butyl tin(TBT) and duron on marine periphyton communities detected as pollution-induced community tolerance. Arch. Environ. Contam. Toxicol. 22: 419-427 https://doi.org/10.1007/BF00212562
  36. Nicholas F.H. and Thompson J.K. 1982. Seasonal growth in the bivalve Macoma balthica near the southern limit of its range. Estuaries 5: 110-120 https://doi.org/10.2307/1352108
  37. O'Connor T.P. 1992. Recent trends in coastal environmental quality: results from the first five years of the NOAA mussel watch project. US Department of Commerce, NOAA, National Ocean Service, Washington DC
  38. Okamoto O.K., Asano C.S., Aidar E. and Colepicolo P. 1996. Effects of cadmium on growth and superoxide dismutase activity of the marine microalga Tetraselmis gracilis (Prasinophyceae). J. Phycol. 32: 74-79 https://doi.org/10.1111/j.0022-3646.1996.00074.x
  39. Ouzounidou G. 1994. Copper-induced changes on growth metal content and photosynthetic function of Alyssum montanum L. plant. Environ. Exp. Bot. 34: 165-172 https://doi.org/10.1016/0098-8472(94)90035-3
  40. Peters C.E., Grassman J.J., Firman J.C., Richmond R.H. and Power E.A. 1997. Ecotoxicology of tropical marine ecosystems. Enoironm, Toxicol. Chem. 16: 12-40 https://doi.org/10.1897/1551-5028(1997)016<0012:EOTME>2.3.CO;2
  41. Petersen S. and Gustavson K. 2000. Direct toxic effects of TBT on natural enclosed phytoplankton at ambient TBT concentrations of coastal waters. Ecotoxicolgy 9: 273-285 https://doi.org/10.1023/A:1026570011420
  42. Pflugmacher S., Schwarz S., Pachur H.J. and Steinberg C.E.W. 2000. Effects of tributyltin chloride (TBTCI) on detoxication enzymes in aquatic plants. Environ. Toxicol. 15: 225-233 https://doi.org/10.1002/1522-7278(2000)15:3<225::AID-TOX7>3.0.CO;2-F
  43. Phillips D.J.H. 1990. Use of macroalgae and invertebrates as monitors of metal levels in estuarines and coastal waters. In: Furness R.W. and Rainbow P.S. (eds), Heavy metals in the marine environment. CRC Press, Boca Raton, Florida, pp. 81-99
  44. Phillips D.J.H. 1993. Macrophytes as biomonitors of trace metals. In: Kramer K.J.M. (ed), Biomonitoring of coastal waters and estuaries. CRC Press, Boca Raton, Florida, pp. 85-103
  45. Platt T., Gallegos C. & Harrison W.G. 1980. Photoinhibition of photosynthesis in natural assemblage of marine phytoplankton. J. Mar. Res. 38: 687-701
  46. Prasad M.N.V. and Strzalka K. 1999. Impact of heavy metals on photosynthesis. In: Prasad M.N.V. and Hagemeyer J. (eds), Heavy metal stress in plants. Springer, Berlin. pp. 177-138
  47. Price A.R.G. and Coles S.L. 1992. Aspects of seagrass ecology along the western Arabian Gulf-Coast. Hydrobiologia 234: 129-141 https://doi.org/10.1007/BF00014245
  48. Rainbow M.H.J., Phillips D.J.H. and Depledge M.H. 1990. The significance of trace metal concentrations in marine invertebrates: a need for laboratory investigation of accumulation strategies. Mar. Pollut. Bull. 21: 321-324 https://doi.org/10.1016/0025-326X(90)90791-6
  49. Rainbow P.S. and Philips D.J.H. 1993. Cosmopolitan biomonitors of trace metal. Mar. Pollut. Bull. 26: 593-601 https://doi.org/10.1016/0025-326X(93)90497-8
  50. Ralph P.J. and Burchett M.D. 1998. Photosynthetic response of Hatophila ovalis to heavy metal stress. Environ. Pollut. 103: 91-101 https://doi.org/10.1016/S0269-7491(98)00121-3
  51. Schreiber U., Bilger W. and Neubauer C. 1994. Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze E.D. and Caldwell M.M. (eds), Ecophysiology of photosynthesis. Springer, Berlin. pp. 49-70
  52. Shim W.J., Kahng S.H., Hong S.H., Kim N.S., Kim S.K. and Shim J.H. 2000. Imposex in the rock shell Thais clavigera as evidence of organotin contamination in the marine environment of Korea. Mar. Environ. Res. 49: 435-451 https://doi.org/10.1016/S0141-1136(99)00084-7
  53. Short F.T. and Wyllie-Echeverria S. 1996. Natural and human-induced disturbances of seagrasses. Environ. Cons. 23: 17-27 https://doi.org/10.1017/S0376892900038212
  54. Stobart A.K., Griffiths W.T., Ameen-Bukhari I. and Sherwood R.P. 1985. The effect of $Cd^{2+}$ on the biosynthesis of chlorophyll in leaves of barley. Physiol. Plant. 63: 293-298 https://doi.org/10.1111/j.1399-3054.1985.tb04268.x
  55. Terlizzi A., Graschetti S., Gianguzza P., Faimal M. and Boero F. 2001. Environmental impact of antifouling technologies: state of the art and perspectives. Aquatic Conservation: Mar. Freshw. Ecosys. 11: 311-317 https://doi.org/10.1002/aqc.459
  56. Vavilin D.V., Polynov V.A., Matorin D.N. and Venediktov P.S. 1995. Sublethal concentrations of copper stimulate photosystem II photoinhibition in Chlorella pyrenoidosa. Plant Physiol. 146: 609-614 https://doi.org/10.1016/S0176-1617(11)81922-X
  57. Wahbeh M.I. 1984. Levels of zinc, manganese, magnesium, iron and cadmium in three species of seagrasses from Aqaba (Jordan). Aquat. Bot. 20: 179-183 https://doi.org/10.1016/0304-3770(84)90034-2
  58. Wang W.-X. and Dei R.C.H. 1999. Kinetic measurements of metal accumulation in two marine macroalgae. Mar. Biol. 135: 11-23 https://doi.org/10.1007/s002270050596
  59. Ward T.J. 1989. The accumulation and effects of metals in seagrass habitats. In: Larkum A.W.D., McComb A.J. and Shepherd S.A. (eds), Biology of seagrasses: a Treatise on the biology of seagrass with special reference to Australian Region. Elsevier, New York, pp. 797-820
  60. Warnau M., Fowler S.W. and Teyssie J.-L. 1996. Biokinetics of selected heavy metals and radionuclides in two marine macrophytes: the seagrass Posidonia oceanica and the alga Caulerpa taxifolia. Mar. Environ. Res. 41: 343-363 https://doi.org/10.1016/0141-1136(95)00025-9
  61. Webb W.L., Newton M. and Starr D. 1974. Carbon dioxide exchange of Alnus rubra: a mathematical model. Oceologia 17: 281-291 https://doi.org/10.1007/BF00345747
  62. Weckx J.E.J. and Clijsters, H.M.M. 1996. Oxidative damage and defence mechanisms in primary leaves of Phaseolus vulgaris as a result of root assimilation of toxic amounts of copper. Physiol. Plant. 96: 506-512 https://doi.org/10.1111/j.1399-3054.1996.tb00465.x
  63. Zolotukhina E.Y. 1995. Change of microelement balance and photosynthesis of seaweeds under the action of heavy metals. Vest. Moskovsk. Univ. Ser. XVI Biol. 71: 46-54

Cited by

  1. Application of Hydroacoustic System and Kompsat-2 Image to Estimate Distribution of Seagrass Beds vol.17, pp.3, 2012, https://doi.org/10.7850/jkso.2012.17.3.181
  2. Cytotoxic Effect of Zostera asiatica on Growth of Human Cancer Cells vol.27, pp.4, 2012, https://doi.org/10.7841/ksbbj.2012.27.4.227
  3. Inhibitory Effect of Zostera japonica on Growth of Human Cancer Cells vol.34, pp.4, 2012, https://doi.org/10.4217/OPR.2012.34.4.385