Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cell Age Optimization for Hydrogen Production Induced by Sulfur Deprivation Using a Green Alga Chlamydomonas reinhardtii UTEX 90

  • KIM , JUN-PYO (Department of Chemical Engineering, Sungkyunkwan University) ;
  • KANG, CHANG-DUK (School of Chemical Engineering, Seoul National University) ;
  • SIM, SANG-JUN (Department of Chemical Engineering, Sungkyunkwan University) ;
  • KIM, MI-SUN (Biomass Research Team, Korea Institute of Energy Research) ;
  • PARK, TAI-HYUN (School of Chemical Engineering, Seoul National University) ;
  • LEE, DONG-HYUN (Department of Chemical Engineering, Sungkyunkwan University) ;
  • KIM, DUK-JOON (Department of Chemical Engineering, Sungkyunkwan University) ;
  • KIM, JI-HEUNG (Department of Chemical Engineering, Sungkyunkwan University) ;
  • LEE, YOUNG-KWAN (Department of Chemical Engineering, Sungkyunkwan University) ;
  • PAK, DAE-WON (Water Environment and Remediation Research Center, Korea Institute of Science and Technology)
  • Published : 2005.02.01
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Abstract

Under sulfur deprived conditions, PS II and photosynthetic $O_2$ evolution by Chlamydomonas reinhardtii UTEX 90 are inactivated, resulting in shift from aerobic to anaerobic condition. This is followed by hydrogen production catalyzed by hydrogenase. We hypothesized that the photosynthetic capacity and the accumulation of endogenous substrates such as starch for hydrogen production might be different according to cell age. Accordingly, we investigated (a) the relationships between hydrogen production, induction time of sulfur deprivation, increase of chlorophyll after sulfur deprivation, and residual PS II activity, and (b) the effect of initial cell density upon sulfur deprivation. The maximum production volume of hydrogen was 151 ml $H_2$/l with 0.91 g/l of cell density in the late-exponential phase. We suggest that the effects of induction time and initial cell density at sulfur deprivation on hydrogen production, up to an optimal concentration, are due to an increase of chlorophyll under sulfur deprivation.

Keywords

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