Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of a New Fluorescent Pseudomonas Strain that Produces Both Phenazine 1-Carboxylic Acid and Pyoluteorin

  • HU, HONG-BO (College of Life Science and Biotechnology, Shanghai Jiaotong University) ;
  • XU, YU-QUAN (College of Life Science and Biotechnology, Shanghai Jiaotong University) ;
  • FENG CHEN (College of Life Science and Biotechnology, Shanghai Jiaotong University) ;
  • XUE HONG ZHANG (College of Life Science and Biotechnology, Shanghai Jiaotong University) ;
  • HUR, BYUNG-KI (Department of Biological Engineering, Inha University)
  • Published : 2005.02.01
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Abstract

Strain M-18 was isolated from the rhizosphere soil of sweet melon, using 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source. Its phenotypic characteristics, metabolic tests, and 16S rDNA sequence were analyzed. The antibiotics secreted by strain M-18 were determined to be phenazine 1-carboxylic acid and pyoluteorin. These data showed that strain M-18 was a new fluorescent Pseudomonas strain that produced both phenazine 1-carboxylic acid and pyoluteorin, some features being similar to Pseudomonas aeruginosa and Pseudomonas fluorescens. Therefore, the strain M-18 appears to be the first pseudomonad described to date that is capable of producing both phenazine 1-carboxylic acid and pyoluteorin.

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References

  1. Bender, C. L., V. Rangaswamy, and J. Loper. 1999. Polyketide production by plant-associated Pseudomonads. Annu. Rev. Phytopathol. 37: 175-196 https://doi.org/10.1146/annurev.phyto.37.1.175
  2. Bergey, D., G. H. John, R. K. Noel, and H. A. S. Peter. 1994. Bergey's Manual of Determinative Bacteriology. 9th Ed. Lippincott Williams & Wilkins, Philadelphia, U.S.A.
  3. Duffy, B. K. and G. Defago. 1999. Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas Fluorescens biocontrol strains. Appl. Environ. Microbiol. 65: 2429- 2438
  4. Glick, S. R., D. M. Karaturovic, and P. C. Newell. 1995. A novel procedure for rapid isolation of plant growth promoting pseudomonads. Can. J. Microbiol. 41: 533- 536 https://doi.org/10.1139/m95-070
  5. Haas, D., C. Blumer, and C. Keel. 2000. Biocontrol ability of fluorescent pseudo monads genetically dissected: Importance of positive feedback regulation. Curr. Opin. Biotechnol. 11: 290- 297 https://doi.org/10.1016/S0958-1669(00)00098-7
  6. Huang, X. Q., D. H. Zhu, Y. H. Ge, H. B. Hu, X. H. Zhang, and Y. Q. Xu. 2004. Identification and characterization of pltZ, a gene involved in the repression of pyoluteorin biosynthesis in Pseudomonas sp M18. FEMS Microbiol. Lett. 232(2): 197-202 https://doi.org/10.1016/S0378-1097(04)00074-6
  7. Keen, N. T., S. Tarnaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasm ids for DNA cloning in gram-negative bacteria. Gene 70: 191- 197 https://doi.org/10.1016/0378-1119(88)90117-5
  8. King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanine and fluorescein. J. Lab. Clin. Med. 44: 301- 307
  9. Kreig, N. R. and J. G. Holt. 1989. Gram negative rods and cocci. In: Bergey's Manual of Systematic Bacteriology, Williams & Wilkins. Baltimore, U.S.A.
  10. Lanyi, B. 1987. Classical and rapid identification methods for medically important bacteria. Methods Microbiol. 19: 167
  11. Lee, J. Y., S. S. Moon, and B. K. Hwang. 2003. Isolation and in vitro and in vivo activity against Phytophthora capsid and Colletotrichum orhiculare of phenazine-l-carboxylic acid from Pseudomonas aeruginosa strain GC-B26. Pest Manag. Sci. 59: 872- 882 https://doi.org/10.1002/ps.688
  12. Levitch, M. E. and E. R. Stadtman. 1964. A study of the biosynthesis of phenazine-l-carboxylic acid. Arch. Biochem. Biophy. 106: 194- 199 https://doi.org/10.1016/0003-9861(64)90175-4
  13. Mavrodi, D. V., V. N. Ksenzenko, R. F. Bonsall, R. J. Cook, A. M. Boronin, and L. S. Thomashow. 1998. A sevengene locus for synthesisof phenazine-l-carboxylic acid by Pseudomonas fluorescens 2-79. J. Bacteriol. 180: 2541-2548
  14. Nowak-Thompson, B., N. Chaney, J. S. Wing, S. J. Gould, and J. E. Loper. 1999. Characterization of the pyoluteorin biosynthetic gene cluster of Pseudomonas fluorescens Pf-5. J. Bacteriol. 181: 2166-2174
  15. Nowak-Thompson, B., S. J. Gould, J. Kraus, and J. E. Loper. 1994. Production of 2,4-diacetylphloroglucinol by the biocontrol agent Pseudomonasfluorescens Pf-5. Can. J. Microbiol. 40: 1064- 1166 https://doi.org/10.1139/m94-168
  16. Pierson III, L. S., G. Thomas, L. Stephen, and F. Gong. 1995. Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium Pseudomonas aureofaciens 30-84. FEMS Microbiology Lett. 134: 299- 307 https://doi.org/10.1111/j.1574-6968.1995.tb07954.x
  17. Raaijmakers, J. M., M. Vlami, and J. T. Souza. 2002. Antibiotic production by bacterial biocontrol agents. Antonie van Leeuwenhoek 81: 537- 547 https://doi.org/10.1023/A:1020501420831
  18. Sambrook, J., E. F. Fritsch, and A. E. Roodbeen. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor, Cold Spring Harbor Laboratory Press, New York, U.S.A.
  19. Shaukat, S. S. and I. A. Siddiqui. 2003. The influence of mineral and carbon source on biological control of charcoal rot fungus, Macrophomina phaseolina by fluorescent pseudomonads in tomato. Lett. Appl. Microbiol. 36: 392-398 https://doi.org/10.1046/j.1472-765X.2003.01325.x
  20. Slininger, P. J., J. E. Van Cauwenberge, R. J. Bothast, D. M. Weller, L. S. Thomashow, and R. J. Cook. 1996. Effect of growth culture physiological state, metabolites, and formulation on the viability, phytotoxicity, and efficacy of the take-all biocontrol agent Pseudomonas fluorescens 2-79 stored encapsulated on wheat seeds. Appl. Microbiol. Biotechnol. 45: 391- 398 https://doi.org/10.1007/s002530050701
  21. Slininger, P. J. and M. A. Shea-Wilbur. 1995. Liquid-culture pH, temperature, and carbon (not nitrogen) source regulate phenazine productivity of the take-all biocontrol agent Pseudomonasfluorescens 2-79. Appl. Microbiol. Biotechnol. 43: 794- 800 https://doi.org/10.1007/BF02431910
  22. Stover, C,K., X. Q. Pham, A. L. Erwin, S. D. Mizoguchi, P. Warrener, and M. J Hickey, et al. 2000. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature 406: 959-964 https://doi.org/10.1038/35023079
  23. Thomashow, L. S. 1996. Biological control of plant root pathogens. Curr. Opin. Biotechnol. 7: 343- 347 https://doi.org/10.1016/S0958-1669(96)80042-5