약학회지 (YAKHAK HOEJI)

  • 제49권2호
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  • Pages.128-133
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  • 2005
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  • 0377-9556(pISSN)
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  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Diclofenac에 의해 유발된 장내세균전위와 지질과산화에 대한 글루타민의 효과

Effect of Glutamine on the Diclofenac Induced Bacterial Translocation and Lipid Peroxidation

  • 김은정 (숙명여자대학교 약학대학) ;
  • 김정욱 (중앙대학교 의과대학 내과학교실)
  • Kim, Eun-Jeong (College of Pharmacy, Sookmyung Women's University) ;
  • Kim, Jeong-Wook (Department of Internal Medicine, Chungang University of College of Medicine)
  • 발행 : 2005.01.01
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초록

The aim of this study was to examine whether administration of glutamine are able to prevent the NSAID induced bacterial translocation and lipid peroxidation in the rats. The an imals with glutamine were fed with L-glutamine for 5 days before diclofenac administration (100 mg/kg orally). 48 hour after diclofenac administration, intestinal permeability, serum biochemical profiles, and malondialdehyde levels of ileum were measured for evaluation of gut damage. Also, enteric aerobic bacterial counts, number of gram-negatives in mesenteric Iymph nodes, liver, spleen and kidney and malondialdehyde levels in liver, spleen, kidney and plasma were measured. Diclofenac caused the gut damage, enteric bacterial overgrowth, increased bacterial translocation and increased lipid peroxidation. Co-administration of glutamine reduced the gut damage, enteric bacterial overgrowth, bacterial translocation and lipid peroxidation induced by diclofenac. This study suggested that glutamine might effectively prevent non-steroidal anti-inflammatory drug induced bacterial translocation and lipid peroxidation in the rat.

키워드

참고문헌

  1. Davies, N. M. : Review article: non-steroidal anti-inflammatory drug-induced gastrointestinal permeability. Aliment. Pharmacol. Ther. 12, 303 (1998) https://doi.org/10.1046/j.1365-2036.1998.00300.x
  2. Allison, M. C., Howatson, A. G., Torrance, C., Lee F. D. and Russell, R. I. : Gastrointestinal damage associated with the use of non-steroidal anti-inflammatory drugs. N. Engl. J. Med. 327, 749 (1992) https://doi.org/10.1056/NEJM199209103271101
  3. Bjarnason, I., Zanelli, G., Smith, T., Prouse, P., Williams, P., Smethurst, P., Delacey; G., Gumpel, M. J. and Levi, A. J. : Nonsteroidal anti-inflammatory drug-induced intestinal inflammation in humans. Gastroenterology 93, 480 (1987) https://doi.org/10.1016/0016-5085(87)90909-7
  4. Page, J. and Henry; D, : Consumption of NSAIDs and the development of congestive heart failure in elderly patients: an underrecognized public health problem. Arch. Intern. Med. 160, 777 (2000) https://doi.org/10.1001/archinte.160.6.777
  5. Rauchhaus, M.,Sharma, R. and Bolger, A. : NSAIDs, intestinal cell integrity, and bacterial translocation in chronic heart failure. Arch. Intern. Med. 160, 3004 (2000) https://doi.org/10.1001/archinte.160.19.3004
  6. Brinkmann, A., Wolf, C. E, Berger, D., Kneitinger, E., Neumeister, B., Buchler, M., Radermacher, P., Seeling, W. and Georgieff, M. : Perioperative endotoxemia and bacterial translocation during major abdominal surgery: evidence for the protective effect of endogenous prostacyclin? Crit. Care. Med. 24, 1293 (1996) https://doi.org/10.1097/00003246-199608000-00005
  7. Wiest, R. and Rath, H. C. : Gastrointestinal disorders of the critically ill. Bacterial translocation in the gut. Best. Pract. Res. Clin. Gastroenterol. 17, 397 (2003) https://doi.org/10.1053/bega.2002.0349
  8. Basivireddy; J., Vasudevan, A., Jacob, M. and Balasubramanian, K. A. : Indomethacin-induced mitochondrial dysfunction and oxidative stress in villus enterocytes. Biochem. Pharmacol. 64, 339 (2002) https://doi.org/10.1016/S0006-2952(02)01067-5
  9. Basivireddy, J., Jacob, M., Pulimood, A. B. and Balasubramanian, K. A. : Indomethacin-induced renal damage: role of oxygen free radicals. Biochem. Pharmacol. 67, 587 (2004) https://doi.org/10.1016/j.bcp.2003.09.023
  10. Galati, G., Tafazoli, S., Sabzevari, O., Chan, T. S. and O'Brien, P. J. : Idiosyncratic NSAID drug induced oxidative stress. Chem. Biol, Interact. 142, 25 (2002) https://doi.org/10.1016/S0009-2797(02)00052-2
  11. Alonso de Vega, J. M., Diaz, J., Serrano, E. and Carbonell, L. F.: Oxidative stress in critically ill patients with systemic inflammatory response syndrome. Crit. Care. Med. 30, 1782 (2002) https://doi.org/10.1097/00003246-200208000-00018
  12. Eleftheriadis, E., Kotzampassi, K, Papanotas, K, Heliadis, N. and Sarris, K. : Gut ischemia, oxidative stress, and bacterial translocation in elevated abdominal pressure in rats. World. J. Surg. 20, 11 (1996) https://doi.org/10.1007/s002689900151
  13. Chiva, M., Soriano, G., Rochat, I., Peralta, C., Rochat, F., Llovet, T., Mirelis, B., Schiffrin, E. J., Guarner, C. and Balanzo, J. : Effect of Lactobacillus johnsonii Lal and antioxidants on intestinal flora and bacterial translocation in rats with experimental cirrhosis. J. Hepatol. 37, 456 (2002) https://doi.org/10.1016/S0168-8278(02)00169-1
  14. Ocal, K., Avlan, D., Cinel, I., Unlu, A., Ozturk, C., Yaylak, F., Dirlik, M., Camdeviren, H. and Aydin, S. : The effect of N-acetylcysteine on oxidative stress in intestine and bacterial translocation after thermal injury. Burns 30, 778 (2004) https://doi.org/10.1016/j.burns.2004.05.006
  15. Buchman, A. L. : Glutamine: commercially essential or conditionally essential? A critieal appraisal of the human data. Am. J. Clin. Nutr. 74, 25 (2001) https://doi.org/10.1093/ajcn/74.1.25
  16. Kudsk, K. A., Wu, Y, Fukatsu, K, Zarzaur, B. L., Johnson, C. D., Wang, R. and Hanna, M. K. : Glutamine-enriched total parenteral nutrition maintains intestinal interleukin-4 and mucosal immunoglobulin A levels. J. Parenten Enteral. Nutr. 24, 270 (2000) https://doi.org/10.1177/0148607100024005270
  17. Jung, S. E., Youn, Y. K., Lim, Y. S., Song, H. G., Rhee, J. E. and Suh, G. J. : Combined administration of glutamine and growth hormone synergistically reduces bacterial translocation in sepsis. J. Korean. Med. Sci. 18, 17 (2003) https://doi.org/10.3346/jkms.2003.18.1.17
  18. Yeh, S. L., Shang, H. F., Lin, M. T., Yeh, C. L. and Chen, W. J. : Effects of dietary glutamine on antioxidant enzyme activity and immune response in burned mice. Nutrition 19, 880 (2003) https://doi.org/10.1016/S0899-9007(03)00159-X
  19. Arndt, H., Kullmann, F., Reuss, F, Scholmerich, J. and Palitzsch, K. D. : Glutamine attenuates leukocyte-endothelial cell adhesion in indomethacin-induced intestinal inflammation in the rat. JPEN. J. Parenter. Enteral. Nutr. 23, 12 (1999) https://doi.org/10.1177/014860719902300112
  20. Hond, E. D., Peeters, M., Hiele, M., Bulteel, V., Ghoos, Y. and Rutgeerts, P. : Effect of glutamine on the intestinal permeability changes induced by indomethacin in humans. Aliment. Pharmacol. Ther. 13, 679 (1999) https://doi.org/10.1046/j.1365-2036.1999.00523.x
  21. Nakamura, J., Takada, S., Ohtsuka, N., Heya, T., Yamamoto, A., Kimura, T. and Sezaki, H. : An assessment of indomethacin-induced gastrointestinal mucosal damage in-vivo: enhancement of urinary recovery after oral administration of phenolsulfonphthalein in rats. J. Pharm. Pharmacol. 35, 369 (1983) https://doi.org/10.1111/j.2042-7158.1983.tb02958.x
  22. Buege, J. A. and Aust, S. D. : Microsomal lipid peroxidation. Method Enzymol. 52, 302 (1978) https://doi.org/10.1016/S0076-6879(78)52032-6
  23. Bradford, M. M. : A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248 (1976) https://doi.org/10.1016/0003-2697(76)90527-3
  24. Atchison, C. R., West, A. B., Balakumaran, A., Hargus, S. J., Pohl, L. R., Daiker, D. H., Aronson, J. F., Hoffmann, W. E., Shipp, B. K. and Treinen-Moslen, M. : Drug enterocyte adducts: possible causal factor for diclofenac enteropathy in rats. Gastroenterology 119, 1537 (2000) https://doi.org/10.1053/gast.2000.20186
  25. Reuter; B. K., Davies, N. M. and Wallace, J. L. : Nonsteroidal anti-inflammatory drug enteropathy in rats: role of permeability, bacteria, and enterohepatic circulation. Gastroenterology 112, 109 (1997) https://doi.org/10.1016/S0016-5085(97)70225-7
  26. Choi, S. H., Lee, S. and Lee, M. D. : Glutamine on the luminal microbial environment after massive small bowel resection. J. Korean. Med. Sci. 17, 778 (2002) https://doi.org/10.3346/jkms.2002.17.6.778
  27. Miller; A. L. : Therapeutic considerations of L-glutamine: a review of the literature. Altern. Med. Rev. 4, 239 (1999)