Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Inhibition of Cell Proliferation and Induction of Apoptosis by Diallyl Disulfide in Human Colon Cancer Cell lines

사람 대장암 세포주에서 Diallyl Disulfide의 세포증식억제 및 Apoptosis 유도 효과

  • Kim Tae Myoung (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University) ;
  • Ryu Jae Myun (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University) ;
  • Kwon Hyun Jung (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University) ;
  • Woo Koan Sik (Department of Food Science and Technology, College of Agriculture, Chungbuk National University) ;
  • Jeong Heon Sang (Department of Food Science and Technology, College of Agriculture, Chungbuk National University) ;
  • Hong Jin Tae (College of Pharmacy, Chungbuk National University) ;
  • Kim Dae Joong (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungbuk national University)
  • 김태명 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 류재면 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 권현정 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 우관식 (충북대학교 농과대학 식품공학과) ;
  • 정헌상 (충북대학교 농과대학 식품공학과) ;
  • 홍진태 (충북대학교 약학대학) ;
  • 김대중 (충북대학교 수의과대학 및 동물의학연구소)
  • Published : 2005.12.01
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Abstract

Epidemiological and laboratory studies provide insight into the anti-carcinogenic potential of garlic and its constituent compounds. Garlic is appealing as an anti-carcinogenic agent due to its ability to induce apoptosis in vitro. Diallyl disulfide (DADS) is one of the major components of garlic that used to determine inhibition of cell proliferation and induced apoptosis in human colon cell lines. In this study, human colorectal cancer cell lines (LOVO, HCT-116, SW-480) were exposed to DADS. The inhibitory effects of DADS dose level more than $50\;{\mu}M$ in the cell viability of all cell lines. Cell growth activity inhibits of human colon cancer cell lines. The inhibitory effects of DADS dose level more than $25\~50\;{\mu}M$ in the cell growth using MTT assay. We found that DADS may have the apoptosis action (chromatin condensation, DNA fragmentation) using DAPI staining and increased the expression of caspase-3 at the dose level more than $100\;{\mu}M$, decreased the expression level of $\beta-catenin$ at dose dependent in the western blotting. We suggest that DADS may have a potential candidate as cancer chemopreventive agents.

Keywords

References

  1. Barone, F.E. and Tansey, M.R. (1977): Isolation, purification, identifycation, synthesis and kinetics of activity of the anticandidal component of Allium sativum and a hypothesis for its mode of action. Mycologia., 69, 713-825 https://doi.org/10.2307/3758870
  2. Block, E. (1992): The organosulfur chemistry of genus Alliumimplications for the organic chemistry of sulfur. Angw Chem. Int. Ed. Engl., 31,1135-1178 https://doi.org/10.1002/anie.199211351
  3. Challier, B., Perarnau, J.M. and Viel, J.F. (1998): Garlic, onion and cereal fibre as protective factors for breast cancer: a French case-control study. Eur. J. Epidemiol., 14, 737-747 https://doi.org/10.1023/A:1007512825851
  4. Chung, J.G., Lu, H.F., Yeh, C.C., Cheng, K.C., Lin, S.S. and Lee, J.H. (2004): Inhibition of N-acetyltransferase activity and gene expression in human colon cancer cell lines by diallyl sulfide. Fd. Chem. Toxico., 42, 195-202 https://doi.org/10.1016/j.fct.2003.08.015
  5. Evans, V.G. (1993): Multiple pathways to apoptosis. Cell Biol. Int., 17, 461-476 https://doi.org/10.1006/cbir.1993.1087
  6. Fleischauer, A.T., Poole, C. and Arab, L. (2000): Garlic consumption and cancer prevention: meta-analyses of colorectal and stomach cancers. Am. J. Clin. Nutr., 72, 1047-1052 https://doi.org/10.1093/ajcn/72.4.1047
  7. Fleischauer, A.T. and Arab, L. (2001): Garlic and cancer: a critical review of epidemiologic leteratrure. J. Nutr., 131, 1032-1040 https://doi.org/10.1093/jn/131.3.1032S
  8. Hudson, E.A, Howells, L.M., Gallacher-Horley, B., Fox, L.H., Gescher, A. and Manson, M.M. (2003): Growth-inhibitory effects of the chemopreventive agent indole-3-carbinol are increased in combination with the polyamine putrescine in the SW480 colon tumour cell line. BMC Cancer, 3, 2 https://doi.org/10.1186/1471-2407-3-2
  9. Kim, D.J., Shin, D.H., Ahn, B., Kang, J.S., Nam, K.T., Park, C.B., Kim, C.K., Hong, J.T., Kim, Y.B., Yun, Y.W., Jang, D.D. and Yang, K.H. (2003): Chemoprevention of colon cancer by Korean food plant components. Mutat. Res., 524. 99-107 https://doi.org/10.1016/S0027-5107(02)00325-1
  10. Kim, D.J., Kang, J.S., Ahn, B., Kim, K.S., Park, K.H., Choi, K.S., Surh, Y.J. and Kim, N.D. (2001): Chemopreventive effect of 2-(allylthio)pyrazine (2-AP) on rat colon carcinogenesis induced by azoxymethane (AOM). Cancer Lett., 166,125-33 https://doi.org/10.1016/S0304-3835(01)00408-6
  11. Kim, D.J. (1998): Dietary factors and cancer prevention. Cancer Preven. Res., 3, 24-39
  12. Kitagawa, M., Hatakeyama, S., Shirane, M., Matsumoto, M., Ishida, N., Hattori, K., Nakamichi, I., Kikuchi, A., Nakayama, K. and Nakayama, K. (1999): An F-box protein, FWD1, mediates ubiquitin-dependent proteolysis of beta-catenin. EMBO J., 18, 2401-2410 https://doi.org/10.1093/emboj/18.9.2401
  13. Klipstein-Grobusch, K., Kroke, A., Voss, S. and Boeing, H. (1998): Influence of lifestyle on the use of supplem ents in the Brandenburg nutrition and cancer study. Z. Ernahrungswiss., 37, 38-46 https://doi.org/10.1007/PL00007371
  14. Korinek, V., Barker, N., Morin, P.J., Van Wichen, D., Weger, R.D., Kinzler, K.W., Vogelstein, B. and Clevers, H. (1997): Constitutive transcriptional activation by a $\beta$-catenin-Tcf complex in APC-/- colon carcinoma. Science, 275, 1784-1787 https://doi.org/10.1126/science.275.5307.1784
  15. Kolligs, F.T., Nieman, M.T., Winer, I., Hu, G., Van Mater, D., Feng, Y, Smith, I.M., Wu, R, Zhai, Y., Cho, K.R and Fearon, E.R (2002): ITF-2, a downstream target of the Wnt/TCF pathway, is activated in human cancers with beta-catenin defects and promotes neoplastic transformation. Cancer Cell, 1, 145-155 https://doi.org/10.1016/S1535-6108(02)00035-1
  16. Kwon, S.K (2003): Organosulfur compounds from Allium sativum and physiological activities. J. Appl. Pharmacol., 11, 8-32
  17. Kweon, S.H., Park, K.A. and Choi, H.M. (2003): Chemopreventive effect of garlic powder diet in diethylnitrosamine-induced rat hepatocarcinogengesis. Life Sci., 73, 2515-2526 https://doi.org/10.1016/S0024-3205(03)00660-X
  18. Morin, P.J. (1999): Beta-catenin signaling and cancer. BioEssays, 21, 1021-1030 https://doi.org/10.1002/(SICI)1521-1878(199912)22:1<1021::AID-BIES6>3.0.CO;2-P
  19. Nagata, S. (1997): Apoptosis by death factor. Cell, 88, 355-365 https://doi.org/10.1016/S0092-8674(00)81874-7
  20. Ocker, M., Herold, C., Ganslmayer, M., Hahn, E.G. and Schuppan, D. (2003): The synthetic retinoid adapalene inhibits proliferation and induces apoptosis in colorectal cancer cells in vitro. Int. J. Cancer, 107, 453-459 https://doi.org/10.1002/ijc.11410
  21. O'Gara, EA, Hill, D.J. and Maslin, D.J. (2000): Activities of garlic oil, garlic powder and their diallyl constituents against Helicobacter pylori. Appl. Environ. Microbiol., 20, 14-18
  22. Oommen, S., Anto, R.J., Srinivas, G. and Karunagaran, D. (2004): Allicin (from garlic) induces caspase-mediated apoptosis in cancer cells. Euro. J. Pharmacol., 485, 97-103 https://doi.org/10.1016/j.ejphar.2003.11.059
  23. Sengupta, A., Ghosh, S. and Das, S. (2004): Modulatory influence of garlic and tomato on cycloaxygenase-2 activity, cell proliferation and apaptasis during azoxymethane induced colon carcinogenesis in rat. Cancer Lett., 208, 127-136 https://doi.org/10.1016/j.canlet.2003.11.024
  24. Steinmetz, K.A., Kushi, L.H., Bostick, R.M., Folsom, A.R. and Poter, J.D. (1994): Vegetables, friut and colon cancer in the Iowa Women's Health Study. Am. J. Epidemiol., 139, 1-15 https://doi.org/10.1093/oxfordjournals.aje.a116921
  25. Surh, Y.J. (2003): Cancer chemoprevention with dietary phytochemicals. Nature Rev., 3, 711-780 https://doi.org/10.1038/nrc1206
  26. Wong, N.A. and Pignatelli, M. (2002): Beta-catenin: a linchpin in colorectal carcinogenesis? Am. J. Pathol., 160, 389-401 https://doi.org/10.1016/S0002-9440(10)64856-0