한국환경성돌연변이발암원학회지 (Environmental Mutagens and Carcinogens)
- 제25권4호
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- Pages.134-142
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- 2005
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- 1225-6307(pISSN)
Microarray Analysis of the Gene Expression Profile in Diethylnitrosamine-induced Liver Tumors in Mice
- Jung Eun-Soo (College of Veterinary Medicine, Seoul National University) ;
- Park Jung-Duck (College of Medicine, Chung-Ang University) ;
- Ryu Doug-Young (College of Veterinary Medicine, Seoul National University)
- 발행 : 2005.12.01
초록
Liver cancer is a leading cause of tumor-related mortality, Diethylnitrosamine (DEN) is one of the most extensively studied hepatic carcinogens to date. In this study, the mRNA expression profile in DEN-induced liver tumors in mice was analyzed using DNA microarrays. We report increased expression of genes that participate in hypoxia response, including metallothionein 1 (Mt1), metallothionein 2 (Mt2), fatty acid synthase (Fasn), transferrin (Trf), adipose differentiation-related Protein (AdfP) and ceruloplasmin (CP), as well as those involved in predisposition and development of cancers, such as cytochrome P450 2A5 (Cyp2a5), alpha 2-HS-glycoprotein (Ahsg) and Jun-B oncogene (Junb). The hepatic iron regulatory peptide, hepcidin (Hampl), was downregulated in DEN-stimulated liver tumors. Expression of tumor suppressor genes, such as tripartite motif protein 13 (Trim13), was decreased under these conditions. The data collectively indicate that DEN-induced tumor development can be exploited as a possible model for liver cancer, since this process involves various genes with important functions in hepatic carcinogenesis.