The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effect of Green Tea Powder on Growth of Lactic Culture

가루녹차가 요구르트 균주의 증식에 미치는 영향

  • Jung Da-Wa (Dept. of Food and Nutrition, Kyungwon University) ;
  • Nam Eun-Sook (Dept. of Food and Nutrition, Kyungwon University) ;
  • Park Shin-In (Dept. of Food and Nutrition, Kyungwon University)
  • 정다와 (경원대학교 식품영양학과) ;
  • 남은숙 (경원대학교 식품영양학과) ;
  • 박신인 (경원대학교 식품영양학과)
  • Published : 2005.12.01
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Abstract

This experiment was carried out to investigate the effect of green tea powder on the growth and acid production of lactic culture in reconstituted skim milk. The supplementation level of green tea powder to reconstituted skim milk was $0.5\%,\;1.0\%,\;1.5\%,\;2.0\%\;and\;2.5\%$. Reconstituted skim milk containing green tea powder was fermented by single or mixed culture of Streptococcus thermophilus, Lactobacillus acidophilus and Lactobacillus casei. Supplementation of green tea powder did not significantly stimulate growth and acid production of Streptococcus thermophilus and Lactobacillus casei. But the growth and acid production of Lactobacillus acidophilus were slightly enhanced by the addition of green tea powder. When green tea powder was added to reconstituted skim milk at the level of $0.5\%\;or\;1.0\%$, all mixed cultures oi lactic acid bacteria showed high number of viable cell count and the acid production than 3 kinds of single cultures. Therefore, it was suggested to manufacture the yoghurt with the addition of $0.5\~1.0\%$ green tea powder and the inoculation of mixed cultures of lactic acid bacteria for on the stimulation of growth of the lactic culture.

가루녹차를 첨가한 기능성 요구르트를 개발하기 위한 기초 연구로 가루녹차의 농도별 첨가가 요구르트 균주의 증식 및 산 생성에 미치는 영향을 조사하였다. 가루녹차를 첨가한 skim milk 배지에 Streptococcus thor-mophilus, Lactobacillus acidophilus, Lactobacillus casei 를 단독 균주 및 혼합 균주로 접종하여 배양하면서 생 균수와 pH 및 적정산도를 측정하였다. 단독균주의 경우 가루녹차의 첨가 농도($0.5\%,\;1.0\%,\;1.5\%,\;2.0\%\;and\;2.5\%$)를 달리하였을 때 streptococcus thermophilus와 Lactobacillus casei는 가루녹차 첨가에 의해 증식 효과는 아주 미비하였으나 억제 효과가 나타나지 않았으며, Lactobacillus acidophilus는 배양 9시간부터 가루녹차 첨가구에서 대조구에 비해 높은 균수를 보이며 약간의 생육 촉진 효과를 보였고, 배양 12시간부터 pH가 하락하였고 적정산도는 상승하였다. 그러나 가루녹차의 첨가량에 따른 생육과 산 생성 정도에 미치는 영향의 차이는 뚜렷하지 않았다. 혼합 균주의 경우, 단독균주로 배양하였을 때보다 가루녹차에 의한 유산균의 증식이 촉진되었다. Streptococcus thermophilus와 Lac-tobacillus acidophilus는 가루녹차 $0.5\%$와 $1.0\%$ 첨가시, 배양 15시간 후에 각각 $1.5\times10^9$ CFU/mL, 또한 Streptococcus thermophilus와 Lactobacillus casei는 가루녹차 $0.5\%$ 첨가시 배양 12시간 후 $1.4\times10^9$ CFU/mL, $1.0\%$ 첨가시에는 배양 15시간 후에 $1.5\times10^9$ CFU/mL로 최대 균수를 나타내었으며, pH 저하와 적정산도 상승이 뚜렷하였다. 따라서 가루녹차 첨가 기능성 요구르트 제조시 가루녹차를 $0.5\~l.0\%$ 첨가하여 유산균을 혼합균주로 사용하는 것이 이들 유산균의 증식에 가장 적합한 것으로 나타났다.

Keywords

References

  1. Park, CO, Jin, SH and Ryu, BH. Antioxidant activity of green tea extracts toward human low density lipoprotein. Kor. J. Food Sci. Technol. 28:850-858. 1996
  2. Kim, BS, Yang, WM and Choi, J. Comparison of , caffeine, free amino acid, vitamin C and catechins content of commercial green tea in Bosung, Sunchon, Kwangyang and Hadong. J. Kor. Tea Soc. 8:55-62. 2002
  3. Kang, WS, Lee, YH, Chung, HH, Kang, MK, Kim, TJ, Hong, JT and Yun, YP. Effects of green tea catechins on the lipid peroxidation and superoxide dismutase. J. Food Hyg. Safety. 16:41-47. 2001
  4. Song, HS, Lee, HK and Kang, MH. Antimutagenic effects of water extracts of persimmon leaf tea, green tea and oolong tea on reversion and survival of selected Salmonella tester strains. J. Kor. Soc. Food Sci. Nutr. 28:599-606. 1999
  5. Park, JS, Shin, MK, Sohn, HS, Park, RK, Kim, MS and Jeong, WH. Green tea (- )EGCG induces the apoptotic death of lung cancer cells via activation of c-Jun N-terminal kinase 1 and activating protein-1. Kor. J. Nutr. 35:53-59. 2002
  6. Park, GY, Lee, SJ and Im, JG. Effects of green tea catechin on cytochrome $P_{450}$, xanthine oxidase activities in liver and liver damage in streptozotocin induced diabetic rats. J. Kor. Soc. Food Sci. Nutr. 26: 901-907. 1997
  7. Chung, HC and Yoo, YS. Effects of aqueous green tea extracts with a tocopherol and lecithin on the lipid metabolism in serum and liver of rats. Kor. J. Nutr. 28:15-22. 1995
  8. Choi, SI, Lee, JH and Lee, SR. Effect of green tea beverage for the removal of cadmium and lead by animal experiments. Kor. J. Food Sci. Technol. 26: 745-749. 1994
  9. Choi, SH, Kim, SH and Lee, BH. Effect of green tea on the anti-duodenal ulcer in cysteamine administrated rats. J. Kor. Soc. Food Nutr. 22:374-380. 1993
  10. Ahn, HY, Lee, MY and Yun, YP. The effects of green tea catechins on vascular smooth muscle tension and $^{45}Ca^{2+}$ uptake. J. Food Hyg. Safety. 11:83-87. 1996
  11. Shin, DH and Lee, YW. Effect of green tea powder on the sensory quality of cooked rice. Kor. J. Food Nutr. 17:266-271. 2004
  12. Kwon, MY, Lee, YK and Lee, HG. Sensory and mechanical characteristics of heunmi-nokcha-injulmi supplemented by green tea powder. Kor. J. Home Economics. 34:329-339. 1996
  13. Choi, EH and Kim, MK. Effects of different moisture addition and sugar on the quality of nokcha-julpyun. Kor. J. Food Culture. 18:28-36. 2003
  14. Hong, HJ, Choi, JH, Yang, JA, Kim, GY and Rhee, SJ. Quality characteristics of seo1giddeok added with green tea powder. Kor. J. Soc. Food Sci. 15:224-230. 1999
  15. Yun, GY and Kim, MA. The effect of green tea powder on yackwa quality and preservation. Kor. J. Food Culture. 20:103-112. 2005
  16. Kim, HS and Kim, SN. Effects of addition of green tea powder and Angelica keiskei powder on the quality characteristics of yukwa. Kor. J. Soc. Food Cookery Sci. 17:246-254. 2001
  17. Kim, JS. Sensory characteristics of green tea bread. Kor. J. Food Nutr. 11:657-661. 1998
  18. Hwang, YK, Hyun, YH and Lee, YS. Study on the characteristics of bread with green tea powder. Kor. J. Food Nutr. 14:311-316. 2001
  19. Im, JG and Kim, YH. Effect of green tea addition on the quality of white bread. Kor. J. Soc. Food Sci. 15:395-400. 1999
  20. Hwang, SY, Choi, OK and Lee, HJ. Influence of green tea powder on the physical properties of the bread flour and dough rheology of white pan bread. Kor. J. Food Nutr. 14:34-39. 2001
  21. Hyun, YH, Hwang, YK and Lee, YS. A study of cooking properties of the noodle made of composite flour with green tea powder. J. East Asian Soc. Dietary Life. 11:295-304. 2001
  22. Park, JH, Kim, YO, Kug, YI, Cho, DB and Choi, HK. Effects of green tea powder on noodle properties. J. Kor. Soc. Food Sci. Nutr. 32:1021-1025. 2003 https://doi.org/10.3746/jkfn.2003.32.7.1021
  23. Kim, AJ, Lim, YH, Kim, MH and Kim, MW. Quality characteristics of mungbean starch gels added with green tea powder. J. East Asian Soc. Dietary Life. 12: 135-140. 2002
  24. Jung, JY and Cho, EJ. The effect of green tea powder levels on storage characteristics of tofu. Kor. J. Soc. Food Cookery Sci. 18:129-135. 2002
  25. Nha, YA and Park, JN. Effect of dried powders of pine needle, pine pollen, green tea and horseradish on preservation of kimchi-yangnyum. Kor. J. Culinary Research. 9:179-190. 2003
  26. Park, MJ, Jeon, YS and Han, JS. Fermentation characteristics of mustard leaf kimchi added green tea and pumpkin powder. J. Kor. Soc. Food Sci. Nutr. 30: 215-221. 2001
  27. Park, GS, Park, EJ and Kim, HH. Quality characte- ristics of green tea powder on mayonnaise. J. East Asian Soc. Dietary Life. 10:411-418. 2000
  28. Choi, SH, Kwon, HC, An, DJ, Park, JR and Oh, DH. Nitrite contents and storage properties of sausage added with green tea powder. Kor. J. Food Sci. Ani. Resour. 23:299-308. 2003
  29. Park, GS, Lee, SJ and Jeong, ES. The quality characteristics of beef jerky according to the kinds of saccharides and the concentrations of green tea powder. J. Kor. Soc. Food Sci. Nutr. 31:230-235. 2002 https://doi.org/10.3746/jkfn.2002.31.2.230
  30. Bang, BH and Park, HH. Preparation of yogurt added with green tea and mugwort tea and quality characteristics. J. Kor. Soc. Food Sci. Nutr. 29:854-859. 2000
  31. Collins, CH and Lyne, PM. Microbiological Methods. 5th ed., Bufferworths, London, UK. 1984
  32. Wee, JH and Park, KH. Retardation of kimchi fermentation and growth inhibition of related microorganism by tea catechins. Kor. J. Food Sci. Technol. 29:1275-1280. 1997
  33. Im, KS. Effect of fermented milk on human health. Kor. J. Food Nutr. 16:93-103. 2003