The Effect of Disregarding of Heat Detection on Embryo Production in Superovulation of Hanwoo

한우 과배란처리시 CIDR 처리와 미처리가 수정란 생산에 미치는 영향

  • Choi S. H. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Son D. S. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Ryu I. S. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Cho S. R. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Han M. H. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Kim H. J. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Choe C. Y. (Animal Genetic Resources Station, NLRI, RDA) ;
  • Kim Y. K (Animal Genetic Resources Station, NLRI, RDA)
  • 최선호 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 손동수 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 류일선 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 한만희 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김현종 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 최창용 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김영근 (농촌진흥청 축산연구소 가축유전자원시험장)
  • Published : 2005.12.01

Abstract

This study was conducted to investigate the effects of disregarding of heat detection on embryo production in superovulation of Hanwoo cows. Donors which showed 1 or 2 times of normal heat and had no abnormality of reproductive tract were selected The superovulation was performed injection of 2.5 mL FSH (Antorin R-10, Japan) 2 times on 5 days before next heat and continuously with the reduction of dose to 0.5 mL of first injection 2 times in a day for next 3 days. Otherwise, the donors of CIDR group were inserted CIDR plus (with the capsule of estradiol benzoate 10 mg) on Day 10 from standing heat for 9 days. On 6 days from insertion of CIDR, FSH was injected above same manners. The response according to the natural heat and CIDR were $82.2\%,\;89.7\%$, respectively. There were no difference between both treatments. The recovery rates of embryos were 7.7, 10.5, respectively and transferable embryos were 3.4, 3.8. There showed significant difference between both treatments (p<0.05). These results suggested that disregarding of heat detection in superovulation could be produced transferable embryos for embryo transfer and preserve the donors from the excess hormonal administration and maintain the economical lift span of genetically available Hanwoo donors.

본 연구는 한우를 공란우로 이용시 효율을 최대화하고, 과도한 호르몬의 투여를 억제하며, 그로 인하여 공란우의 경제수명을 연장하고자 과배란 처리시 차기 발정을 고려하지 않고 과배란을 유도하였을때 공란우의 반응과 수정란의 생산율을 조사하였으며, 그 결과는 다음과 같다. 1. 한우 경산우 및 미경산우를 자연발정을 확인하고 차기발정 5일전에 FSH를 1일 2회 4일 간 투여한 것과 발정을 확인하지 않고 CIDR를 이용하여 동일한 호르몬을 방법으로 과배란을 처리하였을 때, 반응율은 각각 $82.2\%,\;89.7\%$였으며, 유의적인 차이는 인정되지 않았다. 2. 한우 경산우 및 미경산우를 자연발정을 확인하고 차기 발정 5일전에 FSH를 1일 2회 4일간 투여한 것과 발정을 확인하지 않고 CIDR를 이용하여 동일한 호르몬을 같은 방법으로 과배란을 처리하였을 때, 수정란의 회수율은 각각 7.7개, 10.5개를 회수하였고, 그 중 이식가능 수정란은 각각 3.4개, 3.8개를 나타냈으며, 두 처리간에 유의적인 차이를 나타냈다(p<0.05). 이상의 결과로 한우 공란우는 경산우 및 미경산우를 발정주기를 확인하지 않고도 과배란처리시 반응을 확인할 수 있었으며, 수정란의 회수에 있어서도 적절하게 채취할 수 있으므로 한우를 공란우로서 과도한 호르몬으로부터 보호할 수 있으며, 그에 따라 경제수명을 연장할 수 있을 것으로 사료된다. 또한 고능력 한우를 이용한 수정란 이식의 활성화에 유용한 방법이며, 농가에도 확산하여 소득향상에 기여할 수 있을 것으로 기대된다.

Keywords

References

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