Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Relationship between HSP70 Gene Polymorphisms and IVF Embryo Development in Pigs

돼지에서 HSP70 유전자형과 IVF 수정란 배 발달과의 관련성

  • Jin H. J. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Kim I. C. (Swine Science Division, NLRI, RDA) ;
  • Wee M. S. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Yeon S. H. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Kim C. D. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Cho C. Y. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Choi S. H. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Cho S. R. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Son D. S. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Kim Y. K. (Animal Genetic Resources Station, National Livestock Research Institute, RDA) ;
  • Jung J. H. (Animal Science and Biotechnology, Seoul National University) ;
  • Choi H. S. (Course of Pet Beauty Fashion Design, Kimcheon College) ;
  • Park C. K. (College of Animal Resource Science, Kangwon National University)
  • 진현주 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김인철 (농촌진흥청 축산연구소 양돈과) ;
  • 위미순 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 연성흠 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김종대 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 조창연 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 최선호 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 조상래 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 손동수 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 김영근 (농촌진흥청 축산연구소 가축유전자원시험장) ;
  • 정종현 (서울대학교 동물생명공학) ;
  • 최화식 (김천대학 애완동물뷰티패션디자인과) ;
  • 박춘근 (강원대학교 동물자원과학대학)
  • Published : 2005.12.01
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Abstract

This study was performed to investigate the relationship between Heat shock protein 70 (HSP70) gene polymorphism and in vitro fertilization(IVF) embryo development in the pigs. The single strand conformation polymorphism(SSCP) genotypes from HSP70 K1, K3 and K4 PCR products were detected different patterns. In cleavage rate of oocyte fertilized in vitro, HSP70 K1-AA genotype($73.1\%$) and K1-AB genotype($62.3\%$) showed significantly higher oocyte cleavage rate than HSP70 K1-BB genotype($49.3\%$)(p<0.05). And HSP70 K3-AA genotype ($72.4\%$) and K3-AB($62.2\%$) also showed significantly higher oocyte cleavage rate than HSP70 K3-BB genotype($49.1\%$)(p<0.05). The IVF embryo development of 2-cell stage according to HSP70 genotypes of sperm and pig breeds also showed a significant difference. The number of embryos developed to 2-cell stage in Landrace(28.8) and Duroc(29.8) were significantly higher than in Yorkshire(10.9)(p<0.05). And also HSP70 K4-AB genotype group(29.6) higher than HSP70 K4-AA genotype group(10.6)(p<0.05). However, the number of embryos developed to blastocyst stage did not showed significant differences among breeds as well as HSP70 genotypes. These resrults suggest that in vitro development in porcine early embryos may be affected by HSP70 genotypes and breeds.

본 연구는 돼지에서 스트레스 관련 HSP70 유전자의 다형성과 IVF 수정란의 배발달간의 관련성을 조사하기 위하여 수행되었으며 그 결과는 다음과 같다. HSP70-K1, -K3 및 -K4의 PCR 산물로부터 SSCP 다형성은 각각 다르게 확인되었다. 체외수정란의 분할율 시험에서 HSP70 K1-AA($73.1\%$) 및 K1-AB($62.3\%$) 난할율은 HSP70 K1-BB($49.3\%$)보다 유의적으로 높게 나타났다(p<0.05). 또한 HSP70 K3-AA($72.4\%$) and K3-AB($62.2\%$)형도 HSP70 K3-BB($49.1\%$)형보다 유의적으로 높았다(p<0.05). 돼지품종들과 정자의 HSP70 유전자형에 따른 2-cell 단계까지의 체외수정란 배발달은 유의적 차이를 보였다. Landrace(28.8)와 Duroc(29.8)에서 2세포기까지 발달된 수정란의 수는 Yo.kshire(10.9) 보다 유의적으로 높았다(p<0.05). 또한 HSP70 K4-AB(29.6)형의 2세포기 단계까지의 배발달 수정란 수는 HSP70 K4-AA(10.6)형보다 유의적으로 높았다(p<0.05). 그러나 돼지 품종 및 HSP70 유전자형에 따른 배반포기까지 수정란 배발달 정도는 유의적 차이는 없었다. 이러한 결과들은 돼지 체외수정란의 초기 배발달은 HSP70 유전자형과 품종에 따라서 영향을 받는 것으로 확인되었다.

Keywords

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