Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Chromatin-remodeling Factor INI1/hSNF5/BAF47 Is Involved in Activation of the Colony Stimulating Factor 1 Promoter

  • Pan, Xuefang (Institute of Genetics and Cytology, School of Life Science, Northeast Normal University) ;
  • Song, Zhaoxia (Medical Genetics Department of Basic Medical School, Jilin University) ;
  • Zhai, Lei (Institute of Genetics and Cytology, School of Life Science, Northeast Normal University) ;
  • Li, Xiaoyun (Institute of Genetics and Cytology, School of Life Science, Northeast Normal University) ;
  • Zeng, Xianlu (Institute of Genetics and Cytology, School of Life Science, Northeast Normal University)
  • Received : 2005.03.04
  • Accepted : 2005.05.31
  • Published : 2005.10.31
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Abstract

INI1/hSNF5/BAF47 is a core component of the hSWI/ SNF ATP-dependent chromatin remodeling complex, and it has been implicated in regulating gene expression, cell division and tumorigenesis. We investigated whether INI1/hSNF5/BAF47 functions in activation of the colony stimulating factor 1 (CSF1) promoter in HeLa cells. Overexpression of INI1/hSNF5/BAF47 promoted CSF1 transcription, and siRNA targeting INI1/hSNF5/ BAF47 (siINI1) strongly inhibited the activity of the CSF1 promoter. We demonstrated that all conserved domains of INI1/hSNF5/BAF47 are needed for CSF1 transcription. ChIP experiment showed that INI1/ hSNF5/BAF47 is recruited to the region of the CSF1 promoter. Taken together, these results indicate that INI1/hSNF5/BAF47 is involved in activation of the CSF1 promoter.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China

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