Korean Chemical Engineering Research

The Korean Institute of Chemical Engineers (한국화학공학회)
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Recovery of Aglycone of Daidzein and Genistein in Soybeans

콩에 포함된 비배당체 다이드제인과 제니스테인의 회수

  • Lee, Kwang Jin (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Jin, Long Mei (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University) ;
  • Kim, Young Sik (Department of Chemical Engineering, Samcheok National University) ;
  • Row, Kyung Ho (Center for Advanced Bioseparation Technology and Department of Chemical Engineering, Inha University)
  • 이광진 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 김룡매 (인하대학교 화학공학과, 초정밀생물분리기술연구센터) ;
  • 김영식 (국립삼척대학교 화학공학과) ;
  • 노경호 (인하대학교 화학공학과, 초정밀생물분리기술연구센터)
  • Received : 2005.05.18
  • Accepted : 2005.09.07
  • Published : 2005.10.31
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Abstract

Using aqueous solution of 60% ethanol, the isoflavones contained in the Soybean (Chungsun, Korea), Tawon (Suwon, Korea), Jackbean (Hongsung, Korea), Soybean (Jilin, China), Black Soybean (Jilin, China), Kidneybean (Jilin, China) and Soybean (California, U.S.A.) were extracted. In this work, the pretreatment step was composed of agitation extraction, filtration, concentration, ultracentrifuge, and membrane filtration. The analysis by $C_{18}$ column was performed, and the mobile phase applied was linearly changed with A/B of 85/15-65/35 vol% for 50 min (A water/acetic acid, 99.9/0.1 vol%, B acetonitrile/acetic acid, 99.9/0.1 vol%). Among the soybeans tested, the total amounts of the four isoflavones (daidzin, genistin, daidzein, and genistein) extracted by the soybeans from Korea, China and U.S.A. were 1.37, 1.60, and 2.25 mg/g, respectively. It was also found that the total amount of aglycone of daidzein and genistein from soybean (American California) was $144{\mu}g/g$, which was the largest for the soybeans experimented.

60% 수용성 에탄올 이용하여 대두콩(정선, 한국), 다원콩(수원, 한국), 작두콩(홍성, 한국), 대두콩(길림, 중국), 검정콩(길림, 중국), 붉은 강낭콩(길림, 중국), 대두콩(캘리포니아, 미국)에 포함된 이소플라본을 추출하였다. 본 연구에서의 전처리 단계는 교반추출, 여과, 농축, 원심분리, 막분리로 구성되었다. HPLC 분석은 $C_{18}$ 칼럼을 사용하였고, 이동상 조성은 A는 물/아세트산, 99.9/0.1 vol%, B는 아세토니트릴/아세트산, 99.9/0.1 vol%이며, A/B를 85/15-65/35 vol%로 50분 동안 선형적으로 변화시켰다. 이 중에서 한국산, 중국산, 미국산 대두콩에서 4개의 이소플라본(다이드진, 제니스틴, 다이드제인, 제니스테인)의 추출량은 각각 1,374.09, 1604.53, $2257.41{\mu}g/g$이었다. 또한, 비배당체 이소플라본인 다이드제인과 제니스테인의 추출량은 미국산 대두콩에 $144.06{\mu}g/g$으로 실험한 7개 콩 중에서 가장 많이 포함되었다.

Keywords

Acknowledgement

Supported by : 초정밀생물분리기술연구센터

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