BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Cloning, Identification and Characteristics of a Novel Isoform of Carbamyl Phosphate Synthetase I in Human Testis

  • Huo, Ran (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Zhu, Hui (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Lu, Li (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Ying, Lanlan (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Xu, Min (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Xu, Zhiyang (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Li, Jianmin (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Zhou, Zuomin (Laboratory of Reproductive Medicine, Nanjing Medical University) ;
  • Sha, Jiahao (Laboratory of Reproductive Medicine, Nanjing Medical University)
  • Published : 2005.01.31
Download PDF
⟨ Previous Next ⟩

Abstract

A gene coding a novel isoform of carbamyl phosphate synthetase I (CPS1) was cloned from a human testicular library. As shown by cDNA microarray hybridization, this gene was expressed at a higher level in human adult testes than in fetal testes. The full length of its cDNA was 3831 bp, with a 3149 bp open reading frame, encoding a 1050-amino-acid protein. The cDNA sequence was deposited in the GenBank (AY317138). Sequence analysis showed that it was homologous to the human CPS1 gene. The putative protein contained functional domains composing the intact large subunit of carbamoyl phosphate synthetase, thus indicated it has the capability of arginine biosynthesis. A multiple tissue expression profile showed high expression of this gene in human testis, suggesting the novel alternative splicing form of CPS1 may be correlated with human spermatogenesis.

Keywords

References

  1. Adnan, M. (1970) Effect of arginine on oligospermia. Fertil. Steril. 21, 217-219
  2. Cheng, L. J., Zhou, Z. M., Li, J. M., Zhu, H., Zhu, H., Zhou, Y. D., Wang, L. R., Lin, M. and Sha, J. H. (2002) Expression of a novel HsMCAK mRNA splice variant, tsMCAK gene, in human testis. Life. Sci. 71, 2741-2757 https://doi.org/10.1016/S0024-3205(02)02079-9
  3. Diatchenko, L., Lukyanov, S., Lau, Y. F. and Siebert, P. D. (1999) Suppression subtractive hybridization: a versatile method for identifying differentially expressed genes. Methods. Enzymol. 303, 349-380 https://doi.org/10.1016/S0076-6879(99)03022-0
  4. Eddy, E. M. (1998) Regulation of gene expression during spermatogenesis. Semin. Cell. Dev. Biol. 9, 451-457 https://doi.org/10.1006/scdb.1998.0201
  5. Eickhoff, H., Schuchhardt, J., Ivanov, I, Meier-Ewert, S., O'Brien J., Malik, A., Tandon, N., Wolski, E. W., Rohlfs, E., Nyarsik, L., Reinhardt, R., Nietfeld, W. and Lehrach, H. (2000) Tissue gene expression analysis using arrayed normalized cDNA libraries. Genome Res. 10, 1230-1240 https://doi.org/10.1101/gr.10.8.1230
  6. Kaneko, T., Iuchi, Y., Kobayashi, T., Fujii, T., Saito, H., Kurachi, H. and Fujii, J. (2002) The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis. Eur. J. Biochem. 269, 1570-1578 https://doi.org/10.1046/j.1432-1033.2002.02809.x
  7. Krawetz, S. A., Kramer, J. A. and MeCarrey, J. R. (1999) Reprogramming the male gamete genome: a window to successful gene therapy. Gene 234, 1-9 https://doi.org/10.1016/S0378-1119(99)00147-X
  8. Lee, G. T., Ha, H., Lee, H. C. and Cho, Y. D. (2003) Agmatine reduces hydrogen peroxide in mesanginal cells under high glucose conditions. J. Biochem. Mol. Biol. 36, 251-257 https://doi.org/10.5483/BMBRep.2003.36.3.251
  9. Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967-971 https://doi.org/10.1126/science.1354393
  10. Marshall, M., Metzenberg, R. L. and Cohen, P. P. (1961) Physical and kinetic properties of carbamyl phosphate synthetase from frog liver. J. Biol. Chem. 236, 2229-2237
  11. McCarrey, J. R. (1998) Spermatogenesis as a model system for developmental analysis of regulatory mechanisms associated with tissue-specific gene expression. Semin. Cell. Dev. Biol. 9, 459-466 https://doi.org/10.1006/scdb.1998.0199
  12. Meistrich, M. L., Brock, W. A., Grimes, S. R., Platz, R. D. and Hnilica, L. S. (1978) Nuclear protein transitions during spermatogenesis. Fed. Proc. 37, 2522-2525
  13. Meistrich, M. L. (1989) Histone and basic nuclear protein transitions in mammalian spermatogenesis; in Histones and Other Basic Nuclear Proteins, Hnilica L. S., Stein G. S., Stein J. L. (eds.), pp. 165-182, CRC Press, Orlando, USA
  14. Meistrich, M. L. (1993) Nuclear morphogenesis during spermiogenesis; in Molecular Biology of the Male Reproductive System, Kretser D. M. (ed), pp. 67-97, Academic Press, San Diego, USA
  15. Metzenberg, R. L. (1957) Studies on the biosynthesis of carbamyl phosphate. J. Biol. Chem. 299, 1019-1025
  16. Rubino, S. D., Nyunoya, H. and Lusty, C. J. (1986) Catalytic domains of carbamyl phosphate synthetase. Glutaminehydrolyzing site of Escherichia coli carbamyl phosphate synthetase. J. Biol. Chem. 261, 11320-11327
  17. Sha, J., Zhou, Z., Li, J., Yin, L., Yang, H., Hu, G., Luo, M., Chan, H. C. and Zhou K. (2002) Identification of testis development and spermatogenesis-related genes in human and mouse testis using cDNA microarray. Mol. Hum. Reprod. 8, 511-517 https://doi.org/10.1093/molehr/8.6.511
  18. Simmer, J. P., Kelly, R. E., Rinker, A. G. Jr, Scully, J. L. and Evans, D. R. (1990) Mammalian carbamyl phosphate synthetase (CPS). DNA sequence and evolution of the CPS domain of the Syrian hamster multifunctional protein CAD. J. Biol. Chem. 265, 10395-10402
  19. Thoden, J. B., Miran, S. G., Phillips, J. C., Howard, A. J., Raushel, F. M. and Holden, H. M. (1998) Carbamoyl phosphate synthetase: caught in the act of glutamine hydrolysis. Biochemistry 37, 8825-8831 https://doi.org/10.1021/bi9807761
  20. Trotta, P. P., Burt, M. E., Haschemeyer, R. H. and Meister, A. (1971) Reversible dissociation of carbamyl phosphate synthetase into a regulated synthesis subunit and a subunit required for glutamine utilization. Proc. Natl. Acad. Sci. USA 68, 2599-2603 https://doi.org/10.1073/pnas.68.10.2599

Cited by

  1. Genetic, structural and biochemical basis of carbamoyl phosphate synthetase 1 deficiency vol.101, pp.4, 2010, https://doi.org/10.1016/j.ymgme.2010.08.002
  2. Quantitative and qualitative 2D electrophoretic analysis of differentially expressed mitochondrial proteins from five mouse organs vol.13, pp.1, 2013, https://doi.org/10.1002/pmic.201100539
  3. Genomic and proteomic analyses of 1,3-dinitrobenzene-induced testicular toxicity in Sprague–Dawley rats vol.43, 2014, https://doi.org/10.1016/j.reprotox.2013.10.004
  4. Ammonium metabolism in humans vol.61, pp.11, 2012, https://doi.org/10.1016/j.metabol.2012.07.007