Archives of Plastic Surgery

  • 제32권2호
  • /
  • Pages.143-148
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  • 2005
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  • 2234-6163(pISSN)
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  • 2234-6171(eISSN)
대한성형외과학회 (Korean Society of Plastic and Reconstructive Surgeons)
권호 표지

다양한 세포외기질이 배양 골아세포의 이동에 미치는 영향

The Effects of Various Extracellular Matrices on Motility of Cultured MC3T3-E1 Cell

  • 박병윤 (연세대학교 의과대학 성형외과학교실 인체조직복원연구소) ;
  • 서상우 (연세대학교 의과대학 성형외과학교실 인체조직복원연구소) ;
  • 이원재 (연세대학교 의과대학 성형외과학교실 인체조직복원연구소) ;
  • 류창우 (연세대학교 의과대학 성형외과학교실 인체조직복원연구소) ;
  • 나동균 (연세대학교 의과대학 성형외과학교실 인체조직복원연구소) ;
  • 손현주 (연세대학교 의과대학 의학공학교실, BK21 의과학사업단) ;
  • 박종철 (연세대학교 의과대학 의학공학교실, BK21 의과학사업단)
  • Park, Beyoung Yun (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine,) ;
  • Seo, Sang Woo (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine,) ;
  • Lee, Won Jai (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine,) ;
  • Ryu, Chang Woo (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine,) ;
  • Rah, Dong Kyun (Institute for Human Tissue Restoration, Department of Plastic and Reconstructive Surgery, Yonsei University College of Medicine,) ;
  • Son, Hyun Joo (Brain Korea 21 Project for Medical Science, Medical Engineering, Yonsei University College of Medicine) ;
  • Park, Jong Chul (Brain Korea 21 Project for Medical Science, Medical Engineering, Yonsei University College of Medicine)
  • 투고 : 2004.11.09
  • 발행 : 2005.03.10
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초록

Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

키워드

참고문헌

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