Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Identification of a Rice Gene (Bph 1) Conferring Resistance to Brown Planthopper (Nilaparvata lugens Stal) Using STS Markers

  • Kim, Suk-Man (Department of Agronomy, College of Agriculture, Kyungpook National University) ;
  • Sohn, Jae-Keun (Department of Agronomy, College of Agriculture, Kyungpook National University)
  • Received : 2004.08.13
  • Accepted : 2005.04.20
  • Published : 2005.08.31
⟨ Previous Next ⟩

Abstract

This study was carried out to identify a high-resolution marker for a gene conferring resistance to brown planthopper (BPH) biotype 1, using japonica type resistant lines. Bulked segregant analyses were conducted using 520 RAPD primers to identify RAPD fragments linked to the BPH resistance gene. Eleven RAPDs were shown to be polymorphic amplicons between resistant and susceptible progeny. One of these primers, OPE 18, which amplified a 923 bp band tightly linked to resistance, was converted into a sequence-tagged-site (STS) marker. The STS marker, BpE18-3, was easily detectable as a dominant band with tight linkage (3.9cM) to Bph1. It promises to be useful as a marker for assisted selection of resistant progeny in backcross breeding programs to introgress the resistance gene into elite japonica cultivars.

Keywords

Acknowledgement

Supported by : Rural Development Administration

References

  1. Alam, S. N. and Cohen, M. B. (1998) Detection and analysis of QTLs for resistance to brown planthopper, Nilaparvata lugens, in a doubled-haploid rice population. Theor. Appl. Genet. 97, 1370-1379 https://doi.org/10.1007/s001220051031
  2. Ha, W. H., Kim, K. M., and Sohn, J. H. (2000) Detection of resistance gene to brown planthopper (Nilaparvata lugens Stal.) using RFLP and isozyme markers in rice. Korean J. Breed. 32, 319-322
  3. Han, C. U., Lee, C. H., Jang, K. S., Choi, G. J., Lim, H. K., et al. (2004) Identification of rice genes induced in a rice blastresistance mutant. Mol. Cells 17, 462-468
  4. Hirabayashi, H. and Ogawa, T. (1999) Identification and utilization of DNA markers linked to genes for resistance to brown planthopper (BPH) in rice. Recent Adv. Breed. Sci. 41, 71-74
  5. Ishii, T., Brar, D. S., Multani, D. S., and Khush, G. S. (1994) Molecular tagging of genes for brown planthopper resistance and earliness introgressed from O. australiensis into cultivated rice, O. sativa. Genome 37, 217-221 https://doi.org/10.1139/g94-030
  6. Kalode, M. B. and Krishna, T. S. (1979) Varietal resistance to brown planthopper in India; in Brown Planthopper: Threat to Rice Production in Asia, pp. 369, International Rice Research Institute, Manila, Philippines
  7. Kawaguchi, M., Murata, K., Ishii, T., Takumi, S., and Mori, N. (2001) Assignment of a brown planthopper (Nilaparvata lugens Stal) resistance gene bph4 to the rice chromosome 6. Breed. Sci. 51,13-18 https://doi.org/10.1270/jsbbs.51.13
  8. Khush, G. S. and Brar, D. S. (1991) Genetics of resistance to insects in crop plants. Adv. Agron. 45, 223-274 https://doi.org/10.1016/S0065-2113(08)60042-5
  9. Khush, G. S., Brar, D. S., and Hardy, B. (2001) Rice genetics from Mendel to functional genomics. Rice Genet. IV, 3-25
  10. Kim, S. M., Yang, S. J., and Sohn, J. K. (2003) Development of a DNA marker for selection of resistance plants to brown planthopper (BHP) in rice. Korean J. Breed. 35, 277-282
  11. Kwon, Y. S., Kim, K. M., Kim, D. H., Eun, M. Y., and Sohn, J. K. (2002) Marker assisted introgression of quantitative trait loci associated with plant regeneration ability in anther culture of rice (Oryza sativa L.). Mol. Cells 14, 24-28
  12. Liu, G. Q., Yan, H. H., Fu, Q., Qian, Q., Zhang, Z. T., et al. (2001) Mapping of a new gene for brown planthopper resistance in cultivated rice introgressed from Oryza eichingeri. Chin. Sci. Bull. 46, 738-742 https://doi.org/10.1007/BF02901916
  13. Murai, H., Hashimoto, Z., Sharma, P. N., Shimizu, T., Murata, K., et al. (2001) Construction of a high-resolution linkage map of a rice brown planthopper (Nilaparvata lugens Stal) resistance gene bph2. Theor. Appl. Genet. 103, 526-532 https://doi.org/10.1007/s001220100598
  14. Murata, K., Fujiwara, M., Murai, H., Takumi, N., Mori, N., et al. (2000) Bph9, a dominant brown planthopper resistance gene, is located on the long arm of chromosome 12. Rice Genet. Newslett. 17, 84-86
  15. Nair, S., Kumar, A., Srivastava, M. N., and Mohan, M. (1996) PCR-based DNA markers linked to a gall midge resistance gene, Gm4t, has potential for marker-aided selection in rice. Theor. Appl. Genet. 92, 660-665 https://doi.org/10.1007/BF00226086
  16. Sasaki, T., Matsumoto, T., Antonio, B. A., and Nagamura, Y. (2005) From mapping to sequencing, Post sequencing and Beyond. Plant Cell Physiol. 46, 3-13 https://doi.org/10.1093/pcp/pci503
  17. Toth, B., Galiba, G., Fehér, E., Sutka, J., and Snape, J. W. (2003) Mapping genes affecting flowering time and frost resistance on chromosome 5B of wheat. Theor. Appl. Genet. 107, 509-514 https://doi.org/10.1007/s00122-003-1275-3
  18. Weerasena, J. S., Steffenson, B. J., and Falk, A. B. (2004) Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth. Theor. Appl. Genet. 108, 712-719 https://doi.org/10.1007/s00122-003-1470-2
  19. Xu, X. F., Mei, H. W., Luo, L. J., Cheng, X. N., and Li, Z. K. (2002) RFLP-facilitated investigation of the quantitative resistance of rice to brown planthopper (Nilaparvata lugens). Theor. Appl. Genet. 104, 248-253 https://doi.org/10.1007/s00122-001-0777-0
  20. Yeo, U. S. and Sohn, J. K. (1995) Effective screening methods and inheritance of resistance to brown planthopper (Nilaparvata lugens Stal.) in rice. Korean J. Breed. 27, 372-379
  21. Yeo, U. S. and Sohn, J. K. (2001) Linkage analysis between some agronomic traits and resistance gene to brown planthopper in rice. Korean J. Breed. 33, 287-293
  22. Zenbayashi, K., Zenbayashi, T., Ashizawa, T., Tani, T., and Koizumi, S. (2002) Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32. Theor. Appl. Genet. 104, 547-552 https://doi.org/10.1007/s00122-001-0779-y