Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Developmental Relationship of Unsaturated Fatty Acid Composition and Stearoyl-CoA Desaturase mRNA Level in Hanwoo Steers' Muscle

  • Lee, Seung-Hwan (Department of Applied Biochemistry, Konkuk University) ;
  • Yoon, Du-Hak (Department of Biotechnology and Environment, National Livestock Research Institute) ;
  • Choi, Nag-Jin (Department of Biotechnology and Environment, National Livestock Research Institute) ;
  • Hwang, Soo-Han (Department of Applied Biochemistry, Konkuk University) ;
  • Cheong, Eun-Young (Department of Applied Biochemistry, Konkuk University) ;
  • Oh, Sung-Jong (Department of Biotechnology and Environment, National Livestock Research Institute) ;
  • Cheong, Il-Cheong (Department of Biotechnology and Environment, National Livestock Research Institute) ;
  • Lee, Chang-Soo (Department of Applied Biochemistry, Konkuk University)
  • Received : 2004.05.10
  • Accepted : 2004.10.25
  • Published : 2005.04.01
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Abstract

This study was conducted to investigate the developmental relationship between fatty acid composition in different lipid fractions and stearoyl-CoA desaturase (SCD) gene expression in steer muscles during growth. Twenty Hanwoo steers were used at 6, 12, 18, 24 and 30 months of age. Fatty acid composition and SCD mRNA level were analyzed. In the total lipid fraction, developmental profiles of C18:1, as the product of SCD enzyme, and SCD mRNA level were significantly increased between 6 months and 12 months of age. During this period, the percentage of C18:1 increased from 31.9% to 49.5% in the total lipid. The increased C18:1 level was maintained until 30 months of age within the range of 44.8- 49.9%. In contrast, the C18:0 composition decreased with age and this decrease was compensated by the increase of the C18:1. However, the sum of C18:0 and C18:1 was changed before and after 12-month old by a 20% increase. Unlike the C18 fatty acids, the C16 fatty acids such as C16:0 and C16:1 did not show a consistent change with age in steers' muscle. On the other hand, C18:2 proportion as a major polyunsaturated fatty acid in muscle was significantly reduced from 21.1% at 6 months of age to 4.4% at 12-months old and then this reduced level was maintained until 30 months within the range of 7.4-11.4%. As in the C18:1 composition during early stages, a 2-fold significant increase was observed in the $\Delta^9$-desaturase index of C18 fatty acid as a measure of SCD activity, but not in that of C16 fatty acid. Also, the steady-state level of SCD mRNA reached a peak at 12 months of age. Thus, the positive relationship between the C18:1 composition and the $\Delta^9$-desaturase (SCD enzyme) index of C18 fatty acid or SCD mRNA level was demonstrated during growth, but the negative relationship between the C18:2 composition and the above three indices was demonstrated at the same time, indicating that the sharp induction of SCD mRNA may be closely related to the dramatic reduction of C18:2, which is known as a suppressor of SCD gene expression during growth.

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References

  1. Choi, N. J., M. Enser, J. D. Wood and N. D. Scollan. 2000. Effect of breed on the deposition in beef muscle and adipose tissue of dietary n-3 polyunsaturated fatty acids. Anim. Sci. 71:509-519.
  2. Choi,Y. J., Y. H. Prak, W. P. Michael and J. M. Ntambi. 2001. Regulation of stearoyl-CoA desaturase activity by the trans-10, cis-12 isomer of conjugated linoleic acid in HepG2 cells. Biochem. Biophys. Res. Commun. 284:689-693.
  3. Department of Health. 1994. Report on Health and Social Subjects No. 46, Nutritional Aspects of Cardiovascular Disease. HMSO, London.
  4. Folch, J., M. Lees and G. H. Sloane Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
  5. Harfoot, C. G. and G. P. Hazlewood. 1988. Lipid metabolism in the rumen. In The Rumen Microbial Ecosystem, London, New York. Elsevier Appl. Sci. pp. 285-322.
  6. Hecker, A. L., D. A. Cramer and D. F. Hougham. 1975. Compositional and metabolic growth effects in the bovine muscle, subcutaneous and serum total fatty acids. J. Food Sci. 40:144-149.
  7. Kim, C. M., J. H. Kim, T. Y. Chung and K. K. Park. 2004. Effects of Flaxseed Diets on fattening response of Hanwoo cattle: 1. Performance and carcass characteristics. Asian-Aust. J. Anim. Sci. 17(9):1241-1245.
  8. Kim, C. M., J. H. Kim, T. Y. Chung and K. K. Park. 2004. Effects of Flaxseed Diets on fattening response of Hanwoo cattle: 2. fatty acid composition of serum and adipose tissues. Asian-Aust. J. Anim. Sci. 17(9):1246-1254.
  9. Kim, J. H., C. H. Kim and Y. D. Ko. 2002. Influence of dietary addition of dried wormwood (Artemisia sp.) of the performance and carcass characteristics of Hanwoo steers and the nutrient digestibility of sheep. Asian-Aust. J. Anim. Sci. 15(3):390-395.
  10. Kim, Y. M., J. H. Kim, S. C. Kim, H. M. Ha, Y. D. Ko and C. H. Kim. 2002. Influence of dietary addition of dried wormwood (Artemisia sp.) of the performance and carcass characteristics and fatty acid composition of muscle tissues of Hanwoo Heifers. Asian-Aust. J. Anim. Sci. 15(4):549-554.
  11. Lee, K. N., M. W. Pariza and J. M. Ntambi. 1996. Differential expression of hepatic stearoyl-CoA desaturase gene 1 in male and female mice. Biochim. Biophys. Acta. 1304:85-88.
  12. Lee, S. H., D. H. Yoon, S. H. Hwang, E. Y. Cheong, O. H. Kim and C. S. Lee. 2004. Relationship between monounsaturated fatty acid composition and stearoyl-CoA desaturase mRNA level in Hanwoo liver and loin muscle. J. Anim. Sci. Technol. Kor. 46(1):7-14.
  13. Litman, B. J. 1975. Surface distribution of the fatty acid side chains of phosphatidyl ethanolamine in mixed phospholipids vesicles. Biochim. Biophys. Acta. 413:157-162. https://doi.org/10.1016/0005-2736(75)90100-5
  14. Malau-Aduli, A. E. O., B. D. Siebert, C. D. K. Bottema and W. S. Pitchford. 1997. A comparision of the fatty acid composition of triacylglycerols in adipose tissue from Limousin and Jersey cattle. Aust. J. Agric. Res. 48:715-722.
  15. Mandell, I. B., J. G. Buchanan-Smith and C. P. Campbell. 1998. Effect of forge vs. grain feeding on carcass characteristics, fatty acid composition, and beef quality in Limousin-cross steers when time on feed is controlled. J. Anim. Sci. 76:2619-2630.
  16. Ntambi, J. M. 1999. Regulation of stearoly-CoA desaturase by polyunsaturated fatty acids and cholesterol. J. Lipid Res. 40:1549-1558.
  17. Ntambi, J. M. and M. Miyazaki. 2004. Regulation of stearoyl-CoA desaturase and role in metabolism. Prog. Lipid. Res. 43(2):91-104.
  18. Ott, L. 1984. An introduction to statistical methods and data analysis, 2nd edn. PWS, Boston, MA.
  19. Salter, A. M., E. H. Mangiapane, A. J. Bennett, J. S. Bruce, M. A. Billett, K. L. Anderton, C. B. Marenah, N. Lawson and D. A. White. 1998. The effect of different dietary fatty acids on lipoprotein metabolism: concentration-dependent effects of diets enriched in oleic, myristic, palmitic and stearic acids. Br. J. Nutr. 79:195-202.
  20. Siebert, B. D., M. P. Deland and W. S. Pitchford. 1996. Breed differences in the fatty acid composition of subcutaneous and intramuscular lipid of early and late maturing, grain-finished cattle. Aust. J. Agric. Res. 47:943-952 https://doi.org/10.1071/AR9960943
  21. Solver, H. T. and E. Lanza. 1979. Quantitative analysis of food fatty acids by capillary gas chromatography. J. Am. Oil. Chem. Soc. 56:933-937.
  22. Waters, K. M., F. E. Gomez and J. M. Ntambi. 1996. Polyunsaturated fatty acids inhibit Hepatic stearoyl-CoA desaturase-1 gene in diabetic mice. Lipids 31:S33-S36.
  23. Yang, A., T. W. Larsen, S. B. Smith and R. K. Tune. 1999. $\Delta^9$-Desaturase activity in bovine subcutaneous adipose tissue of different fatty acid composition. Lipids 34:971-978.
  24. Zembayashi, M., K. Nishimura, D. K. Lunt and S. B. Smith. 1995. Effect of breed type and sex on the fatty acid composition of subcutaneous and intramuscular lipids of finishing steers and heifers. J. Anim. Sci. 73:3325-3332.

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