Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Use of SSR Markers to Complement Tests of Distinctiveness, Uniformity, and Stability (DUS) of Pepper (Capsicum annuum L.) Varieties

  • Kwon, Yong-Sham (Variety Testing Division, National Seed Management Office, MAF) ;
  • Lee, Je-Min (Department of plant Science, College of Agriculture and Life Sciences, and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Yi, Gi-Bum (Department of plant Science, College of Agriculture and Life Sciences, and Center for Plant Molecular Genetics and Breeding Research, Seoul National University) ;
  • Yi, Seung-In (Variety Testing Division, National Seed Management Office, MAF) ;
  • Kim, Kyung-Min (Institute of Genetic Engineering, Kyungpook National University) ;
  • Soh, Eun-Hee (Variety Testing Division, National Seed Management Office, MAF) ;
  • Bae, Kyung-Mi (Variety Testing Division, National Seed Management Office, MAF) ;
  • Park, Eun-Kyung (Variety Testing Division, National Seed Management Office, MAF) ;
  • Song, In-Ho (Variety Testing Division, National Seed Management Office, MAF) ;
  • Kim, Byung-Dong (Institute of Genetic Engineering, Kyungpook National University)
  • Received : 2005.02.02
  • Accepted : 2005.03.09
  • Published : 2005.06.30
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Abstract

This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

Keywords

References

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