Clinical and Experimental Reproductive Medicine

  • 제32권3호
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  • Pages.231-242
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  • 2005
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

고환 특이적으로 발현되는 탈유비퀴틴효소 HIDE와 HSP90의 상호작용

HIDE, a Testis Specific Deubiquitinating Enzyme, Interacts with HSP90

  • 성민우 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 김명선 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 김용수 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 이숙환 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 이혜진 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 차광열 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소) ;
  • 백광현 (포천중문 의과대학교 생명과학전문대학원, 세포유전자치료연구소, 차병원 여성의학연구소)
  • Seong, Minu (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Kim, Myung-Sun (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Kim, Yong-Soo (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Lee, Sook-Hwan (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Lee, Hey-Jin (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Cha, Kwang Yul (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital) ;
  • Baek, Kwang-Hyun (Graduate School of Life Science and Biotechnology, Cell and Gene Therapy Research Institute, Pochon CHA University, Infertility Medical Center, CHA General Hospital)
  • 발행 : 2005.09.30
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초록

연구목적: 본 연구는 아직 그 기능이 파악되지 않은 탈유비퀴틴효소 중 하나인 HIDE에 대한 기본적인 생화학적 특징과 고환에서의 발현 양상을 파악하고 있다. 연구재료 및 방법: 인간의 HIDE 유전자를 클로닝하여 효소활성이 있는지 세포 외 실험을 통해 확인하였고, 아미노산 서열을 분석하여 진화상 보존된 부분을 찾아 그 기능을 파악한 다음 HSP90과의 상호작용을 공동면역침전반응으로 확인하였다. HIDE의 조직별 발현양상을 파악하기 위해서 인간과 쥐의 RNA 블롯과 쥐의 단백질 블롯을 이용하여 각각 노던 블롯팅과 웨스턴 블롯팅을 수행하여 고환에서 많이 발현된다는 것을 알았고 이 사실을 바탕으로 쥐의 고환을 절개하여 면역조직화학반응으로써 고환 내의 HIDE 단백질의 발현양상을 파악하였다. 결　과: HIDE는 세포 외에서 유비퀴틴 잔기를 제거하는 탈유비퀴틴 활성이 있으나 세포 내에서 전체적인 유비퀴틴 복합체를 줄여주는 효과는 없었다. HIDE는 HSP90이라는 분자 샤페론과 상호작용한다. HIDE의 전사체는 고환에서 가장 많이 발현되며 다른 조직에서도 소량 발현된다. HIDE의 단백질은 웨스턴 블롯상에서 고환에서만 확인되었다. 고환 내에서의 HIDE의 발현양상은 왕성한 감수분열을 하는 정모세포에서 높았으며 지지세포나 정조세포에는 발현되지 않았다. 결　론: HIDE는 분자 샤페론 HSP90과 상호작용하며 고환 내의 감수분열 중인 세포에서 많이 발현되는 것으로 보아 감수분열이나 정자형성에 관여하는 것으로 보인다.

키워드

과제정보

연구 과제 주관 기관 : 보건복지부

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