대한수의학회지 (Korean Journal of Veterinary Research)

  • 제45권1호
  • /
  • Pages.85-91
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  • 2005
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo

  • Jo, Eun-Hye (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Sung-Dae (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Ahn, Nam-Shik (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Won (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Yang, Se-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Joon-Suk (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Hwang, Jae-Woong (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Sung-Hoon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jung-Ran (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Sun-Jung (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Hyun-Kyung (Sam-Hwa Farm) ;
  • Lee, Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • 심사 : 2005.02.21
  • 발행 : 2005.03.25
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Abstract

Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo.

Keywords

Acknowledgement

Supported by : Seoul National University

References

  1. Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW, Yuan J. Human ICE/CED-3 protease nomenclature. Cell 1996, 87, 171 https://doi.org/10.1016/S0092-8674(00)81334-3
  2. Alpaugh ML, Tomlinson JS, Shao ZM, Barsky SH. A novel human xenograft model of inflammatory breast cancer. Cancer Res 1999, 59, 5079-5084
  3. Bargou RC, Daniel PT, Mapara MY, Bommert K, Wagener C, Kallinich B, Royer HD, Dorken B. Expression of the bcl-2 gene family in normal and malignant breast tissue: low bax-alpha expression in tumor cells correlates with resistance towards apoptosis. Int J Cancer 1995, 60, 854-859 https://doi.org/10.1002/ijc.2910600622
  4. Becher H, Schmidt S, Chang-Claude J. Reproductive factors and familial predisposition for breast cancer by age 50 years. A case-control-family study for assessing main effects and possible gene-environment interaction. Int J Epidemiol 2003, 32, 38-48 https://doi.org/10.1093/ije/dyg003
  5. Brady H, Desai S, Gayo-Fung LM, Khammungkhune S, McKie JA, O'Leary E, Pascasio L, Sutherland MK, Anderson DW, Bhagwat SS. Effects of SP500263, a novel, potent antiestrogen, on breast cancer cells and in xenograft models. Cancer Res 2002, 62, 1439-1442
  6. Brekelmans CT. Risk factors and risk reduction of breast and ovarian cancer. Curr Opin Obstet Gynecol 2003 15, 63-68 https://doi.org/10.1097/00001703-200302000-00010
  7. Cho JH, Cho SD, Hu H, Kim SH, Lee SK, Lee YS, Kang KS. The roles of ERK1/2 and p38 MAP kinases in the preventive mechanisms of mushroom Phellinus linteus against the inhibition of gap junctional intercellular communication by hydrogen peroxide. Carcinogenesis 2002, 23, 1163-1169 https://doi.org/10.1093/carcin/23.7.1163
  8. Grube BJ, Eng ET, Kao YC, Kwon A, Chen S. White button mushroom phytochemicals inhibit aromatase activity and breast cancer cell proliferation. J Nutr 2001, 131, 3288-3293
  9. Hawkin RA, Arends MJ, Ritchie AA, Langdon S, Miller WR. Tamoxifen increases apoptosis but does not influence markers of proliferation in an MCF-7 xenograft model of breast cancer. Breast 2000, 9, 96-106 https://doi.org/10.1054/brst.2000.0140
  10. Hayashi M, Nagata A, Endoh D, Arikawa J, Okui T. High sensitivity of thymocytes of LEC strain rats to induction of apoptosis by X-irradiation. J Vet Med Sci 2002, 64, 597-601 https://doi.org/10.1292/jvms.64.597
  11. Hu H, Ahn NS, Yang X, Lee YS, Kang KS. Ganoderma lucidum extract induces cell cycle arrest and apoptosis in MCF-7 human breast cancer cell. Int J Cancer 2002, 102, 250-253 https://doi.org/10.1002/ijc.10707
  12. Jo EH, Hong HD, Ahn NC, Jung JW, Yang SR, Park JS, Kim SH, Lee YS, Kang KS. Modulations of the Bcl-2/Bax family were involved in the chemopreventive effects of licorice root (Glycyrrhiza uralensis Fisch) in MCF-7 human breast cancer cell. J Agric Food Chem 2004, 52, 1715-1719 https://doi.org/10.1021/jf035012t
  13. King MC, Marks JH, Mandell JB. Breast and ovarian cancer risks due to inherited mutations in BRCA1 and BRCA2. Science 2003, 302, 643-646 https://doi.org/10.1126/science.1088759
  14. Kitts DD, Lim KT. Antitumorigenic and cytotoxic properties of an ethanol extract derived from Rhus verniciflua Stokes (RVS). J Toxicol Environ Health A 2001, 64, 357-371 https://doi.org/10.1080/152873901316981330
  15. Kobayashi H, Shirakawa K, Kawamoto S, Saga T, Sato N, Hiraga A, Watanabe I, Heike Y, Togashi K, Konishi J. Rapid accumulation and internalization of radiolabeled herceptin in an inflammatory breast cancer xenograft with vasculogenic mimicry predicted by the contrast-enhanced dynamic MRI with the macromolecular contrast agent G6-(1B4M-Gd)(256). Cancer Res 2002, 62, 860-866
  16. Korsmeyer SJ. Regulators of cell death. Trends Genet 1995, 11, 101-105 https://doi.org/10.1016/S0168-9525(00)89010-1
  17. Kumar S, Kishimoto H, Chua HL, Badve S, Miller KD, Bigsby RM, Nakshatri H. Interleukin-1 alpha promotes tumor growth and cachexia in MCF-7 xenograft model of breast cancer. Am J Pathol 2003, 163, 2531-2541 https://doi.org/10.1016/S0002-9440(10)63608-5
  18. Leon-Blanco MM, Guerrero JM, Reiter RJ, Calvo JR, Pozo D. Melatonin inhibits telomerase activity in the MCF-7 tumor cell line both in vivo and in vitro. J Pineal Res 2003, 35, 204-211 https://doi.org/10.1034/j.1600-079X.2003.00077.x
  19. Tsao AS, Kim ES, Hong WK. Chemoprevention of cancer. CA Cancer J Clin 2004, 54, 150-180 https://doi.org/10.3322/canjclin.54.3.150
  20. Yamamoto I, Nagumo T, Fujihara M, Takahashi M, Ando Y. Antitumor effect of seaweeds. II. Fractionation and partial characterization of the polysaccharide with antitumor activity from Sargassum fulvellum. Jpn J Exp Med 1977, 47, 133-140
  21. Yan X, Chuda Y, Suzuki M, Nagata T. Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci Biotechnol Biochem 1999, 63, 605-607 https://doi.org/10.1271/bbb.63.605
  22. Yuan J, Shaham S, Ledoux S, Ellis HM, Horvitz HR. The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1 beta-converting enzyme. Cell 1993, 75, 641-652 https://doi.org/10.1016/0092-8674(93)90485-9
  23. Ziyaie D, Hupp TR, Thompson AM. P53 and breast cancer. Breast 2000, 9, 239-246 https://doi.org/10.1054/brst.2000.0199