Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
권호 표지

Differentiation and Labeling of Mouse Preadipocytes for Allogenic Transplantation Study

동종이식 연구를 위한 마우스 지방전구세포의 표지 및 분화 방법의 확립

  • Kim, In Ok (Anterogen, Co., Ltd) ;
  • Kim, Taek Seung (Anterogen, Co., Ltd) ;
  • Kim, Mi Hyung (Anterogen, Co., Ltd) ;
  • Hyon, Won Sok (Department of Plastic and Reconstructive Surgery, Samsung Medical Center, Sungkyunkwan University, College of Medicine) ;
  • Mun, Goo Hyun (Department of Plastic and Reconstructive Surgery, Samsung Medical Center, Sungkyunkwan University, College of Medicine) ;
  • Oh, Kap Sung (Department of Plastic and Reconstructive Surgery, Samsung Medical Center, Sungkyunkwan University, College of Medicine) ;
  • Bang, Sa Ik (Department of Plastic and Reconstructive Surgery, Samsung Medical Center, Sungkyunkwan University, College of Medicine)
  • 김인옥 (주식회사 안트로젠) ;
  • 김택승 (주식회사 안트로젠) ;
  • 김미형 (주식회사 안트로젠) ;
  • 현원석 (성균관대학교 의과대학 삼성의료원 성형외과학교실) ;
  • 문구현 (성균관대학교 의과대학 삼성의료원 성형외과학교실) ;
  • 오갑성 (성균관대학교 의과대학 삼성의료원 성형외과학교실) ;
  • 방사익 (성균관대학교 의과대학 삼성의료원 성형외과학교실)
  • Received : 2005.02.28
  • Published : 2005.07.10
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Abstract

Due to its safety and softness, autologous fat transplantation has been commonly performed for soft tissue correction. However, the injected fat is absorbed resulting in the reduction of volume of the graft by 40-60% within a few months. Thus, there was an attempt to use adipocytes differentiated from preadipocytes in vitro for transplantation. Differentiated adipocytes were biocompatible and matured with gradual volume increase at transplantation site in clinical study(unpublished data). In addition, they did not induce immune rejection in response to nonself lymphocytes in a mixed lymphocyte reaction(MLR)(unpublished data). The purpose of this study is to differentiate mouse preadipocytes following labeling into adipocytes to establish an animal model for allogenic transplantation. Preadipocytes isolated from inguinal and retroperitoneal fat pad of C57BL/6 mice were proliferated with growth medium by passage 3 and differentiated into adipocytes with different culture conditions after labeled with BrdU. At most suitable conditions, above 90% of preadipocytes were differentiated and BrdU labeling did not affect differentiation rate and function of differentiated adipocytes. These results demonstrate that BrdU-labeled adipocytes resulting from this in vitro differentiation protocol are useful for allogenic transplantation study.

Keywords

References

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