Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Pentachlorophenol impact assessment of haematological parameters in olive flounder, Paralichthys olivaceus

  • Jee, Jung-Hoon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Min, Eun-Young (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Hee-Ju (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Lee, Ok-Hyun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Keum, Yoo-Hwa (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Su-Mi (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Woo, Sung-Ho (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Park, Soo-Il (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kang, Ju-Chan (Department of Aquatic Life Medicine, Pukyong National University)
  • Published : 20050400
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Abstract

The toxic effects of pentachlorophenol (PCP) on haematological parameters in olive flounder (Paralichthys olivaceus) after chronic exposure to dietary PCP (0.1, 0.5 and 1.0 mg/kg diet) for 2, 4 and 6 weeks were studied. A significant decrease in total RBC count, haemoglobin concentration and hematocrit value was noted in fish exposed to PCP compared to the non-exposed fish. The PCP treatment group showed significantly lower concentration of serum total protein and albumin, and significantly higher serum chloride, magnesium and total bilirubin levels compared with those in the control group. However, PCP had no major effects on serum glucose, total cholesterol, phosphate and calcium ions in flounder. These results demonstrated that PCP have induced adverse haematological impacts in the olive flounder, Paralichthys olivaceus. Because we found damages in blood-forming function and disruption in blood homeostasis due to chronic exposure to PCP, it is needed to develop further experimental studies for the risk assessment of this environmental pollutant.

Keywords

References

  1. Abrahamsson, K. and Klick, S.: Degradation of halogenated phenols in anoxic marine sediments. Mar. Pollut. Bull.. 22: 227-233,1991 https://doi.org/10.1016/0025-326X(91)90915-F
  2. Bansal, S. K., Verma, S. R., Gupta, A. K. and Dalela, R. C: Physiological dysfunction of the haemapoetic system in a fresh water teleost, Labeo rohita, following chronic chlordane exposure. Part I -Alteration in certain haematological parameters. Bull. Environ. Contam. Toxicol., 22: 666-673, 1979 https://doi.org/10.1007/BF02027005
  3. Borthwick, P. W. and Schimmel, S. C: Toxicity of pentachloro-phenol and related compounds to early life stages of selected estuarine animals. In Pentachloro-phenol: chemistry pharmacology, and environmental toxicology. Rao, K. R. ed. New York, London, Plenum Press, pp. 141-146. 1978
  4. Campbell, T. and Murru, F.: An introduction to fish hematology. The compendium-Small Animal, 12: 525-533,1990
  5. Chen, X., Tin, D., Hu, S. and Hou, Y.: Immunotoxi-city of pentachlorophenol on macrophage immunity and IgM secretion of the crucian carp {Carassius auratus). Bull. Environ. Contam. Toxicol., 73: 153-60, 2004
  6. Crandall, C. A. and Goodnight, C. J.: The effect of various factors on the toxicity of sodium pentachlorophenate to fish. Limnol. Oceanogr., 4: 53-56,1959 https://doi.org/10.4319/lo.1959.4.1.0053
  7. Crosby, D. G., Beynon, K. I., Greve, P. A., Korte, F., Still, G. G. and Vouk, J. W.: Environmental chemistry of pentachlorophenol. Pure Appl. Chem., 53: 1051-1080,1981
  8. Davis, J. C. and Hoos, R. A.: Use of sodium pen-tachloro-phenate and dehydroabiotic acid as reference toxicants for salmonid bioassays. J. Fish. Res. Board Can., 32:411-416,1975 https://doi.org/10.1139/f75-050
  9. Duncan, D. B.: Multiple-range and multiple F tests. Biometrics, 11: 1-42, 1955 https://doi.org/10.2307/3001478
  10. Eduljee, G.: Eduljee, Secondary exposure to diox-ins through exposure to PCP and its derivatives. Sci. Total Environ., 232: 193-214, 1999 https://doi.org/10.1016/S0048-9697(99)00089-3
  11. Eggens, M. L., Opperhuizen, A. and Boon, J. P.: Temporal variation of CYP1A indices, PCB and 1-OH pyrene concentration in flounder, Platichthys flesus, from the Dutch Wadden Sea. Chemosphere, 33: 1579-1596,1996 https://doi.org/10.1016/0045-6535(96)00277-9
  12. Folmar, L. C: Effects of chemical contaminants on blood chemistry of teleost fish: A bibliography and synopsis of selected effects. Environ. Toxicol. Chem., 12: 337-375,1993 https://doi.org/10.1897/1552-8618(1993)12[337:EOCCOB]2.0.CO;2
  13. Gupta, S., Dalela, R. C. and Saxena, P. K.: Influence of dissolved oxygen levels on acute toxicity of phenolic compounds to fresh water teleost Notopterus notopterus (Pallas). Water Air Soil Pollut., 19: 223-228,1983 https://doi.org/10.1007/BF00599049
  14. Hesser, E. F.: Methods for routine fish haematology. Prog. Fish Cult. 22(4): 164-171,1960 https://doi.org/10.1577/1548-8659(1960)22[164:MFRFH]2.0.CO;2
  15. Hylland, K., Sandvik, M., Skare, J. U., Beyer, J., Egaas, E. and Goksoyr, A.: Biomarkers in flounder (Platichthys flesus): An evaluation of their use in pollution monitoring. Mar. Environ. Res., 42: 223-227,1996 https://doi.org/10.1016/0141-1136(95)00034-8
  16. Inglis, A. and Davis, E. L.: Effects of water hardness on the toxicity of several organic and inorganic herbicides to fish. US Bur. Sport Fish. Wildl. Tech. Pap., 67: 1-22,1972
  17. Jee, J. H., Kim, S. G. and Kang, J. C: Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Par-alichthys olivaceus. J. Exp. Mar. Biol. Ecol., 304:123-136,2004 https://doi.org/10.1016/j.jembe.2003.12.001
  18. Jyothi, B. and Narayan, G.: Certain pesticide-induced carbohydrate metabolic disorders in the serum of freshwater fish Clarias batra-chus (Linn.). Food Chem. Toxicol., 37: 417-421,1999 https://doi.org/10.1016/S0278-6915(99)00020-4
  19. Kang, J. C., Kim S. G. and Jee, J. H.: Long-term sublethal cadmium exposed survival, growth and metabolic rate change in the olive flounder, Paralichthys olivaceus. J. Korean Fish. Soc., 36: 39-43, 2003
  20. Kaplan, A. and Szabo, L. L.: Clinical Chemistry: Interpretation and Techniques. Lea and Febiger, Philadelphia. PA., 1979
  21. Maatela, P., Paasivirta, J., Sarkka, J. and Paukku, R.: Organic chlorine compounds in lake sediments: II. Organically bound chlorine. Chemosphere, 21:1343-1354,1990 https://doi.org/10.1016/0045-6535(90)90039-V
  22. McDonald, D. G. and Milligan, C. L.: Chemical properties of the blood. In: Fish Physiology. Hoar, W. S., Randall, D. J. and Farrell, A. P. (Ed.), Academic Press, San Diego, 55-133, 1992
  23. Payne, J. F., Kiceniuk, J. W., Squires, W. R. and Fletcher, G. L.: Pathological changes in marine fish after a 6-month exposed to petroleum. J. Fish. Res. Board Can., 35: 665-667,1978 https://doi.org/10.1139/f78-116
  24. Rusink, R. G. and Smith, L. L.: The relationship of the 96-hour $LC_{50}$ to the lethal threshold concentration of hexavalent chromium, phenol, and sodium pentachlorophenate for fathead minnows (Pimephales promelas Rafinesque). Trans. Am. Fish. Soc., 104: 567-570,1975 https://doi.org/10.1577/1548-8659(1975)104<567:TROTHL>2.0.CO;2
  25. Saarikoski, I. and Viluksela, M.: Influence of pH on the toxicity of substituted phenols to fish. Arch. Environ. Contam. Toxicol., 10: 747-753,1981
  26. Seiler, J. P.: Pentachlorophenol. Mutat. Res., 257: 27-47,1991 https://doi.org/10.1016/0165-1110(91)90018-Q
  27. Shen, D. S., Liu, X. W. and Feng, H. J.: Effect of easily degradable substrate on anaerobic degradation of pentachlorophenol in an upflow anaerobic sludge blanket (UASB) reactor. J. Hazard. Mater., 119: 239-243, 2005 https://doi.org/10.1016/j.jhazmat.2004.12.024
  28. Sievers, G., Palacios, P., Inostroza, R. and Doelz, H.: Evaluation of the toxicity of 8 insecticides in Salmo salar and the in vitro effects against the isopode parasite, Ceratothoa gaudichaudii. Aquaculture, 134:9-16,1995 https://doi.org/10.1016/0044-8486(95)00026-X
  29. Soimasuo, R., Ilmari, J., Kukkonen, J., Petanen, T, Ristola, T, Oidari, A.; Biomarker responses along a pollution gradient: Effects of pulp and paper mill effluents on caged whitefish. Aquat. Toxicol., 31: 329-345,1995 https://doi.org/10.1016/0166-445X(94)00082-2
  30. Tvedten, H.: The complete blood count and bone marrow examination: General comments and selected techniques. In: Small Animal Clinical Diagnosis by laboratory Methods. Willard. M.D, Tvedten, H. and Turnwald, G.H. (Ed.), Saunders, Philadelphia, pp. 14-35,1989
  31. US EPA (U.S. Environmental Protection Agency). Health Effects Assessment for Pen-tachlorophenol. Environmental Criteria and Assessment Office, Office of Health and Environmental Assessment, Office of Research and Development, Cincinnati, OH. EPA/540/1-86/043.1988