Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Molecular Identification of Adoxophyes honmai (Yasuda) (Lepidoptera: Tortricidae) Based on Mitochondrial COI Gene Sequences

  • Lee, So Young (Department of Plant Medicine, Chungbuk National University) ;
  • Park, Hyungjin (Department of Plant Medicine, Chungbuk National University) ;
  • Boo, Kyung Saeng (Department of Applied Biology and Chemistry, Seoul National University) ;
  • Park, Kyu-Tek (Division of Biological Environment, Kangwon National University) ;
  • Cho, Soowon (Department of Plant Medicine, Chungbuk National University)
  • Received : 2005.01.13
  • Accepted : 2005.04.05
  • Published : 2005.06.30
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Abstract

Molecular identification techniques are used where morphological characters are not useful for distinguishing species that resemble each other closely. The example studied here is the Adoxophyes species complex, in which A. orana (Fischer von $R{\ddot{o}}sslerstamm$) is officially the only known Korean species in the genus Adoxophyes (Lepidoptera: Tortricidae). However there have been suspicions that at least two types of A. orana exist in Korea based on the distribution and range of the host, with A. orana attacking apples and peaches, and another Adoxophyes sp. attacking tea and pears. The latter is presumed to be A. honmai (Yasuda), but the two have remained confused because of their extreme morphological similarity, despite several Asian studies of pheromonal and morphological characteristics. To confirm the occurrence of an Adoxophyes species other than A. orana in Korea, we compared 940 bp of the mitochondrial cytochrome oxidase I (COI) gene from 16 samples of Adoxophyes and found that there is a second Adoxophyes species different from A. orana. Comparison of the different sequences to that of Japanese A. honmai confirmed that they belong to the latter. From the sequence difference between the two Korean species, we were able to develop new PCR primer sets that distinguish them. This molecular identification technique with no enzyme digestion or sequencing step is a convenient and rapid way of differentiating between species that are hard to distinguish morphologically.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation

References

  1. Boo, K. S. (1998) Variation in sex pheromone composition of a few selected lepidopteran species. J. Asia-Pacific Entomol. 1, 17-23 https://doi.org/10.1016/S1226-8615(08)60003-5
  2. Brey, P. T. (2003) Anopheles gambiae genome: perspectives for malaria control. Mol. Cells 15, 133-138
  3. Byun, B. K., Bae, Y. S., and Park, K. T. (1998) Illustrated catalogue of Tortricidae in Korea, IK Ser. 2, pp. 321, Junghaeng- Sa, Korea
  4. Caterino, M. S., Cho, S., and Sperling, F. A. H. (2000) The current state of insect molecular systematics: a thriving Tower of Babel. Annu. Rev. Entomol. 45, 1-54 https://doi.org/10.1146/annurev.ento.45.1.1
  5. Cho, S., Mitchell, A., Regier, J. C., Mitter, C., Poole, R. W., et al. (1995) A highly conserved nuclear gene for low-level phylogenetics: elongation factor-1$\alpha$ recovers morphologybased tree for heliothine moths. Mol. Biol. Evol. 12, 650-656
  6. Guerin, P. M., Arn, H., Buser, H. R., and Charmillot, P. J. (1986) Sex pheromone of Adoxophyes orana: additional components and variability in ratio of (Z)-9- and (Z)-11- tetradecenyl acetate. J. Chem. Ecol. 12, 763-772 https://doi.org/10.1007/BF01012108
  7. Honma, K. (1970) Morphological difference of the smaller tea tortrix, Adoxophyes orana, in Japan. Jpn. J. Appl. Entomol. Zool. 14, 89-94 https://doi.org/10.1303/jjaez.14.89
  8. Honma, K. (1971) Apple type and tea type of the smaller tea tortrix. Agric. Hortic. 46, 1647-1650
  9. Kim, K. J. (1990) Distribution of tea tree insect pest and bionomics on the key insect pest in the tea tree plantation. Theses Col. Chonnam Univ. 35, 1-16
  10. Kim, S. S., Sohn, J.-C., and Cho, S. (2004) A taxonomic revision of Illiberis Walker (Lepidoptera: Zygaenidae: Procridinae) in Korea. Entomol. Res. 34, 235-251 https://doi.org/10.1111/j.1748-5967.2004.tb00119.x
  11. Kou, R., Tang, D. S., Chow, Y. S., and Tseng, H. K. (1990) Sex pheromone components of female smaller tea tortrix moth, Adoxophyes sp. (Lepidoptera: Tortricidae) in Taiwan. J. Chem. Ecol. 16, 1409-1415 https://doi.org/10.1007/BF01021036
  12. Landry, B., Powell, J. A., and Sperling, F. A. H. (1999) Systematics of the Argyrotaenia franciscana (Lepidoptera: Tortricidae) species group: evidence from mitochondrial DNA. Ann. Entomol. Soc. Am. 92, 40-46
  13. Lee, S. C., Kim, D. I., and Kim, S. S. (1993) Ecological characteristics of Adoxophyes sp. at tea tree plantation. Korean J. Appl. Entomol. 32, 279-284
  14. Lee, M. L., Suh, S. J., and Kwon, Y. J. (1999) Phylogeny and diagnostic markers of six Tetranychus species (Acarina: Tetranychidae) in Korea based on the mitochondrial cytochrome oxidase subunit I. J. Asia-Pacific Entomol. 2, 85-92 https://doi.org/10.1016/S1226-8615(08)60035-7
  15. Newcomb, R. D. and Gleeson, D. M. (1998) Pheromone evolution within the genera Ctenopseustis and Planotortrix (Lepidoptera: Tortricidae) inferred from a phylogeny based on cytochrome oxidase I gene variation. Biochem. Syst. Ecol. 26, 473-484 https://doi.org/10.1016/S0305-1978(98)00015-5
  16. Park, K. T., Choe, K. Y., Paik, J. C., and Han, S. C. (1977) Lepidopterous insect pest on apple tree. Korean J. Pl. Prot. 16, 33-39
  17. Potting, R. P., Losel, P. M., and Scherkenbeck, J. (1999) Spatial discrimination of pheromones and behavioral antagonists by the tortricid moths Cydia pomonella and Adoxophyes orana. J. Comp. Physiol. A. 185, 419-425 https://doi.org/10.1007/s003590050402
  18. Rose, T. M., Schultz, E. R., Henikoff, J. G., Pietrokovski, S., McCallum, C. M., et al. (1998) Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences. Nucleic Acids Res. 25, 1628-1635
  19. Sperling, F. A. H. and Hickey, D. A. (1994) Mitochondrial DNA sequence variation in the spruce budworm species complex (Choristoneura: Lepidoptera). Mol. Biol. Evol. 11, 656-665
  20. Sperling, F. A. H., Landry, J. F., and Hickey, D. A. (1995) DNA-based identification of introduced ermine moth specials in North America (Lepidoptera: Yponomeutidae). Ann. Entomol. Soc. Am. 88, 155-162
  21. Swofford, D. L. (1999) PAUP$^{\ast}$. Phylogenetic Analysis Using Parsimony ($^{\ast}$and Other Methods). Version 4.0b10. Sinauer Associates, Sunderland, Massachusetts, USA
  22. Tamaki, Y., Noguchi, H., and Yushima, T. (1971a) Sex pheromone of the summer fruit tortrix: isolation and identification. Kontyu 39, 338-340
  23. Tamaki, Y., Noguchi, H., Yushima, T., and Hirano, C. (1971b) Two sex pheromones of the smaller tea tortrix: isolation, identification, and synthesis. Appl. Entomol. Zool. 6, 139-141
  24. Van der Geest, L. P. S. and Evenhuis, H. H. (1991) World Crop Pests. Tortricid Pests, Vol. 5, pp. 808, Elsevier, Netherlands
  25. Walton, C., Handley, J. M., Kuvangkadilok, C., Collins, F. H., Harbach, R. E., et al. (1999) Identification of five species of the Anopheles dirus complex from Thailand, using allelespecific polymerase chain reaction. Med. Vet. Entomol. 13, 24-32 https://doi.org/10.1046/j.1365-2915.1999.00142.x
  26. Xu, X., Xu, J., and Qu, F. (1998) A diagnostic polymerase chain reaction assay for species A and D of the Anopheles dirus (Diptera: Culicidae) species complex based on ribosomal DNA second internal transcribed spacer sequence. J. Am. Mosquito Control Assoc. 14, 385-389
  27. Yasuda, T. (1956) A revision of the genus Adoxophyes. Tyo to Ga 7, 23-26
  28. Yasuda, T. (1972) The Tortricinae and Sparganothinae of Japan (Lepidoptera: Tortricidae), part I. Bull. Univ. Osaka Prefect., Ser. B. 24, 53-134
  29. Yasuda, T. (1975) The Tortricinae and Sparganothinae of Japan (Lepidoptera: Tortricidae), part II. Bull. Univ. Osaka Prefect., Ser. B. 27, 80-251
  30. Yasuda, T. (1998) The Japanese species of the genus Adoxophyes Meyrick (Lepidoptera, Tortricidae). Trans. Lepid. Soc. Jpn. 49, 159-173
  31. Zhou, J., Qiu, H., and Fu, W. (1997) Summer fruit tortrix Adoxophyes orana should be classified as two subspecies (Lepidoptera: Tortricoidea: Tortricidae). Entomotaxonomia 19, 130-134