Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Newly Developed Microsatellite Markers of Mystus nemurus Tested for Cross-Species Amplification in Two Distantly Related Aquacultured Catfish Species

  • Chan, S.C. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Tan, S.G. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Siraj, S.S. (Department of Biology, Faculty of Science, Universiti Putra Malaysia) ;
  • Yusoff, K. (Department of Microbiology, Faculty of Biotechnology and Biomolecular)
  • Received : 2004.10.11
  • Accepted : 2005.05.01
  • Published : 2005.11.01
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Abstract

The work reported here is an attempt to explore the possibility of DNA microsatellite loci transfer (cross-species amplification) to other economically important aquacultured catfish species other than its source species. A total of 25 new microsatellite loci developed for riverine catfish, Mystus nemurus were successfully cross-amplified in two distantly related catfish species within the suborder Siluroidei. Five out of the 19 loci that successfully cross-amplified in Pangasius micronemus were polymorphic, while for Clarias batrachus, cross-amplification was successful using 17 polymorphic loci. The observed heterozygosities were high for all the three catfishes. The results indicated that microsatellite loci could be as polymorphic in non-source species as in the source species.

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References

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