Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Cloning and Intraspecific Variation of β-Actin 2 Gene from the Hermaphroditic Fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae)

암수동체 어류 점박이송사리 Rivulus marmoratus (Cyprinodontiformes, Rivulidae) β-Actin 2 유전자의 클로닝 및 종내 변이

  • Jung, Sang-Oun (Department of Environmental Science, Graduate School, Hanyang University) ;
  • Lee, Young-Mi (Department of Environmental Science, Graduate School, Hanyang University) ;
  • Lee, Chang Joo (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Lee, Jae-Seong (Department of Environmental Science, Graduate School, Hanyang University)
  • 정상운 (한양대학교 대학원 환경과학과) ;
  • 이영미 (한양대학교 대학원 환경과학과) ;
  • 이창주 (한양대학교 자연과학대학 생명과학과) ;
  • 이재성 (한양대학교 대학원 환경과학과)
  • Received : 2004.12.01
  • Accepted : 2005.02.07
  • Published : 2005.03.31
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Abstract

The full sequence analysis of 16 different $\beta-actin$ genes isolated from a single Rivulus marmoratus was performed. The numbers of isolated $\beta-actin$ genes varied from 1764 to 1769. They showed different amino acid residues at the exon 1 region. We named this new gene R. marmoratus $\beta-actin$ 2 gene. Intraspecific variation of R. marmoratus $\beta-actin$ 2 gene was also examined. Major differences of 16 isolated $\beta-actin$ genes, compared to already reported R. marmoratus $\beta-actin$ gene (GenBank AF168615), were observed in both deduced amino acid sequences (1~2%) and intron 2 sequences (4~5%). In this paper, we confirmed the intraspecific variation of $\beta-actin$ gene in this species.

점박이송사리, Rivulus marmoratus에서 기원된 16개의 ${\beta}-actin$ 유전자의 염기서열 분석 결과 1,764~1,769 bp 범위를 가지는 ${\beta}-actin$ 유전자를 분리하였다. 이는 기존에 보고된 점박이송사리 ${\beta}-actin$ 유전자와 exon 1 및 intron 2지역에서 다소의 차이를 보여 우리는 이를 점박이송사리 ${\beta}-actin$ 2 유전자라 명명하였다. Multiple alignment를 이용하여 유전자 서로간의 차이를 비교한 결과, 점박이송사리 ${\beta}-actin$ 유전자는 variation을 볼 수 있었다. 따라서 본 연구에서는 점박이송사리에 있어 ${\beta}-actin$ 유전자의 종내 변이 (intraspecific variation)를 확인하였다.

Keywords

Acknowledgement

Supported by : 한국과학재단

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