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Choristoneura fumiferana Granulovirus pk-1: A Baculoviral Protein Kinase

  • 발행 : 2005.07.31

초록

Open reading frame (ORF) 3 on the Choristoneura fumiferana granulovirus (ChfuGV), located in the 11 kb fragment of the BamHI genomic bank encodes a predicted 32-kDa putative kinase protein. Bioinformatics analysis on the predicted amino acid sequence of ChfuGV PK-1 revealed the existence of 11 catalytic subdomains. Sequence analysis within the 5'-untranslated region (5'-UTR) of ChfuGV pk-1 indicates the presence of both putative early and late promoter motifs, indicating that pk-1 may be expressed throughout the infection cycle. Promoter sequence analysis reveals that pk-1 is deprived of a TATA box and appears instead to be regulated by other cis-acting transcriptional regulatory elements. Temporal transcription analysis by RT-PCR confirms the appearance of transcripts detected from 2 h p.i. until 72 h p.i. Northern blot hybridization characterizes pk-1 transcription as a 1.2 kb transcript. Homology comparisons reveal that ChfuGV PK-1 protein is most closely related to Phthorimaea operculalla granulovirus (PoGV) with 80% amino acid identity.

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참고문헌

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  1. Autographa californica multiple nucleopolyhedrovirus PK-1 is essential for nucleocapsid assembly vol.443, pp.2, 2013, https://doi.org/10.1016/j.virol.2013.05.025
  2. Characterization of a eukaryotic type serine/threonine kinase in Spodoptera litura nucleopolyhedrovirus-I (SpltNPV-I) vol.128, pp.1-2, 2007, https://doi.org/10.1016/j.virusres.2007.04.021