Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Cytokine Reporter Mouse System for Screening Novel IL12/23 p40-inducing Compounds

  • Im, Wooseok (Department of Bioscience and Biotechnology, Sejong University) ;
  • Kim, Hyojeong (Department of Bioscience and Biotechnology, Sejong University) ;
  • Yun, Daesun (Department of Bioscience and Biotechnology, Sejong University) ;
  • Seo, Sung-Yum (Department of Life Science, Kongju National University) ;
  • Park, Se-Ho (School of Biotechnology, Korea University) ;
  • Locksley, Richard M. (Howard Hughes Medical Institute and Department of Medicine and Microbiology & Immunology, University of California) ;
  • Hong, Seokmann (Department of Bioscience and Biotechnology, Sejong University)
  • Received : 2005.07.06
  • Accepted : 2005.07.13
  • Published : 2005.10.31
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Abstract

Cytokines interleukin (IL) 12 and 23 play critical roles in linking innate and adaptive immune responses. They are members of heterodimeric cytokines, sharing a subunit p40. Although IL12/23 p40 is mainly induced in macrophages and dendritic cells (DCs) after stimulation with microbial Toll-like receptor ligands, methods to monitor the cells that produce IL12/23 p40 in vivo are limited. Recently, the mouse model to track p40-expressing cells with fluorescent reporter, yellow fluorescent protein, has been developed. Macrophages and DCs from these mice faithfully reported p40 induction using the fluorescent marker. Here we took advantage of these reporter mice to screen bio-compounds for p40-inducing activity. After screening hundreds of compounds, we found several extracts inducing IL12/23 p40 gene expression. Treatment of DCs with these extracts induced the expression of MHC class II and co-stimulatory molecules, which implies that these might be useful as adjuvants. Next, the in vivo target immune cells of candidate compounds were examined. The reporter system can be useful to identify cells producing IL12 or IL23 in vivo as well as in vitro. Thus, our cytokine reporter system proved to be a valuable reagent for screening for immunostimulatory molecules and identification of target cells in vivo.

Keywords

Acknowledgement

Supported by : Korea Research Foundation, Ministry of Science & Technology

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