Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
권호 표지

Microarray Profiling of Genes Differentially Expressed during Erythroid Differentiation of Murine Erythroleukemia Cells

  • Heo, Hyen Seok (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Ju Hyun (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Lee, Young Jin (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Sung-Hyun (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Cho, Yoon Shin (Department of Life Science, College of Natural Sciences, Hanyang University) ;
  • Kim, Chul Geun (Department of Life Science, College of Natural Sciences, Hanyang University)
  • Received : 2005.02.01
  • Accepted : 2005.05.17
  • Published : 2005.08.31
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Abstract

Murine erythroleukemia (MEL) cells are widely used to study erythroid differentiation thanks to their ability to terminally differentiate in vitro in response to chemical induction. At the molecular level, not much is known of their terminal differentiation apart from activation of adult-type globin gene expression. We examined changes in gene expression during the terminal differentiation of these cells using microarray-based technology. We identified 180 genes whose expression changed significantly during differentiation. The microarray data were analyzed by hierarchical and k-means clustering and confirmed by semi-quantitative RT-PCR. We identified several genes including H1f0, Bnip3, Mgl2, ST7L, and Cbll1 that could be useful markers for erythropoiesis. These genetic markers should be a valuable resource both as potential regulators in functional studies of erythroid differentiation, and as straightforward cell type markers.

Keywords

Acknowledgement

Supported by : Ministry of Health and Welfare of Korea

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