Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Genetic Variation of Two Isolated Relict Populations of Vaccinium uliginosum L. in Korea

들쭉나무 격리잔존 2개 집단의 유전변이

  • Han, Sang-Don (Division of Genetic Resources, Korea Forestry Research Institute) ;
  • Hong, Yong-Pyo (Division of Genetic Resources, Korea Forestry Research Institute) ;
  • Kwon, Hae-Yun (Division of Genetic Resources, Korea Forestry Research Institute) ;
  • Yang, Byeung-Hoon (Division of Genetic Resources, Korea Forestry Research Institute) ;
  • Kim, Chan-Soo (Warm-temperature Forest Research Center, Korea Forestry Research Institute)
  • 한상돈 (국립산림과학원 유전자원과) ;
  • 홍용표 (국립산림과학원 유전자원과) ;
  • 권해연 (국립산림과학원 유전자원과) ;
  • 양병훈 (국립산림과학원 유전자원과) ;
  • 김찬수 (국립산림과학원 난대산림연구소)
  • Received : 2004.12.16
  • Accepted : 2005.08.04
  • Published : 2005.09.30
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Abstract

In order to provide the molecular genetic information necessary for conservation of bog whortleberry (Vaccinium uliginosum L), one of the rare species in Korea, I-SSR analysis was performed on two populations on Mt. Halla and Mt. Seorak. A total of 68 I-SSR products were observed, and higher level of genetic diversity was observed in Mt. Halla population (S.I.=0.539) than in the Mt. Seorak population (S.I,=0.401). Level of genetic diversity in this species was relatively higher than those in other rare species analysed with I-SSR marker. From the results of AMOVA, exceptionally large proportion of genetic diversity (33.5%) was resulted from genetic difference between two populations, and only 66.5% of the genetic variation was allocated in common among individuals within each population, compared with the results in other long-lived woody species. This remarkably high degree of genetic heterogeneity existed between Mt. Halla and Mt. Seorak populations might suggest that they might be originated from the independent progenitors before the post glacier ages, respectively, and/or that they undergone random genetic drift respectively due to geographical isolation resulted from dramatic changes in environmental conditions after the post glacier ages.

희귀수종 들쭉나무(Vaccinium uliginosum L.)의 유전자원 보존을 위한 분자유전학적 정보를 제공하고자 한라산 및 설악산 정상부에 국소적으로 분포하고 있는 2개 집단을 대상으로 I-SSR 표지자 분석을 수행하였다. 증폭된 I-SSR 산물은 총 68개였고, 유전다양성은 한라산 집단이 0.539로 설악산 집단(0.401) 보다 높았으며, 동일한 표지자로 분석된 타 희귀수종에 비해 상대적으로 높은 유전적 다양성을 보였다. AMOVA 분석 결과 전체 유전변이 중 66.5%가 각 집단 내 개체 간에 공통적으로 존재하며, 33.5%는 집단간 유전적 차이에 기인하는 것으로 추정되어 분석된 두 집단 간에 매우 높은 유전적 분화가 관찰되었다. 지리적으로 격리된 이들 집단 간의 높은 유전적 이질성은 두 집단이 각각 별개의 조상집단으로부터 유래되었을 가능성과 빙하기 이후 급격한 환경변화와 지리적 격리 등으로 인해 각 집단이 유전적 부동과정을 거치며 유전적 이질성이 증가하였을 가능성 등에 의해 설명될 수 있을 것으로 생각된다.

Keywords

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