DOI QR코드

DOI QR Code

Polymorphism of the Promoter Region of Hsp70 Gene and Its Relationship with the Expression of HSP70mRNA, HSF1mRNA, Bcl-2mrna and Bax-AMrna in Lymphocytes in Peripheral Blood of Heat Shocked Dairy Cows

  • Cai, Yafei (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Liu, Qinghua (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Xing, Guangdong (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhou, Lei (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Yang, Yuanyuan (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhang, Lijun (College of Animal Science and Technology, Nanjing Agricultural University) ;
  • Li, Jing (Center for Molecular Recognition, Columbia University) ;
  • Wang, Genlin (College of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2004.07.17
  • Accepted : 2004.11.12
  • Published : 2005.05.01

Abstract

The blood samples were collected from dairy cows at the same milking stage. The single-strand conformation polymorphism (PCR-SSCP) method was used to analyze for polymorphism at the 5'flanking region of the hsp70 gene. The mRNA expression levels of HSP70, HSF1, Bcl-2 and Bax-$\alpha$ at different daily-mean-temperature were analyzed by relative quantitative RTPCR. The DNA content, cell phase and the ratio of apoptosis of lymphocytes in peripheral blood of dairy cattle at different daily-meantemperature were determined by FCM. The PCR-SSCP products of primer pair 1 showed polymorphisms and could be divided into four genotypes: aa, ab, ac, cc, with the cis-acting element (CCAAT box) included. Mutations in the hsp70 5'flanking region (468-752 bp) had different effects on mRNA expression of HSP70, HSF1, Bcl-2 and Bax-$\alpha$. The ac genotypic cows showed higher expressions of HSP70mRNA, HSF1mRNA and Bcl-2mRNA/Bax-$\alpha$mRNA and lower ratio of apoptosis. These mutation sites can be used as molecular genetic markers to assist selection for anti-heat stress cows.

Keywords

References

  1. Ahn, S. G. and D. J. Thiele. 2003. Redox regulation of mammalian heat shock factor 1 is essential for Hsp gene activation and protection from stress. Gene and Development 17:516-528.
  2. Aron, Y., M. Busson, B. S. Polla, D. Dusser, A. Lockhart and E. Swierczewski. 1999. Analysis of hsp70-1 gene polymorphism in allergic asthma. Allergy 54:165-170.
  3. Badola, S., T. K. Bhattacharya and T. K. Biswas. 2004. A comparison on polymorphism of Bata-lactoglobulin gene in Bos Taurus and indicine${\times}$Taurine crossed cattle. Asian-Aust. J. Anim. Sci. 17:733-736.
  4. Block, C. J., C. Chase and P. J. Hansen. 2002. Inheritance of resistance of bovinepreimplantation embryos to heat shock: Relative importance of thematernal versus paternal contribution. Mol. Repro. Dev. 63:32-37.
  5. Chrenck, P., J. Huba and D. Vasicek. 2003. The relation between genetic polymorphism markers and milk yield in brown Swiss cattle imported to Slovakia. Asian-Aust. J. Anim. Sci. 16:1397-1401.
  6. Deguchi, Y. and S. Kishimoto. 1990. Enhanced expression of the heat shock protein gene in peripheral blood mononuclear cells of patients with active systemic lupus erythematosus. Ann. Rheum. Dis. 49:893-895.
  7. Dhillon, V. B., S. McCallum, P. Norton, B. M. Twomey, F. Erkeller-Yuksel and P. Lydyard. 1993. Differential heat shock protein overexpression and its clinical relevance in systemic lupus erythematosus. Ann. Rheum. Dis. 52:436-442.
  8. Favatier, F., L. Bornman, L. E. Hightower, E. Gunther and B. S. Polla. 1997. Variation in hsp gene expression and Hsp polymorphism: do they contribute to differential disease susceptibility and stress tolerance? Cell Stress Chaperones 2:141-155.
  9. Favatier, F., M. R. Jacquier-Sarlin, E. Swierczewski and B. S. Polla. 1999. Polymorphism in the regulatory sequence of the human hsp70-1 gene does not affect heat shock factor binding or heat shock protein synthesis. Cell. Mol. Life Sci. 56:701-708.
  10. Holter, J. B., W. J. West, M. L. McGilliard and A. N. Pell. 1996. Predicting ad libitum dry matter intake and yields of Jersey cows. J. Dairy Sci. 79:912-921.
  11. Holter, J. B., J. W. West and M. L. McGilliard. 1997. Predicting ad libitum dry matter intake and yield of Holstein cows. J. Dairy. Sci. 80:2188-2199.
  12. Lyashko, V. N., V. K. Vikulova, V. G. Chernikov, V. I. Ivanov, K. A. Ulmasov and O. G. Zatsepina. 1994. Comparison of the heat shock response in ethnically and ecologically different human populations. Proc. Natl. Acad. Sci. USA 91:12492-12495.
  13. Milner, C. M. and R. D. Campbell. 1990. Structure and expression of the three MHC-linked Hsp70 genes. Immunogenetics. 32:242-251.
  14. Milner, C. M. and R. D. Campbell. 1992. Polymorphic analysis of three MHC-linked Hsp70 genes. Immunogenetics 36:357-362.
  15. Mosser, D. D., A. W. Caron and L. Bourget. 1997. Role of the human heat shock protein HSP70 in protection against stressinduced apoptosis. Mol. Cell. Biol. 17:5317-5321.
  16. Perfettini, J. L., R. T. Kroemer and G. Kroemer. 2004. Fatal liaisons of p53 with Bax and Bak. Nature Cell Biology. 6:386-388.
  17. Pociot, F., K. S. Ronningen and J. Nerup. 1993. Polymorphic analysis of the human MHC-linked heat shock protein 70 (HSP70-2) and HSP70-Hom genes in insulin-dependent diabetes mellitus (IDDM). Scand. J. Immunol. 338: 491-495.
  18. Ross, T., R. Olivier and L. Monney. 1998. Bcl-2 prolongs cell survival after Bax-induced release of cytochrome. Nature 391:496-499.
  19. Shimizu, S., K. Nomura, M. Ujihara, K. Sakamoto, H. Shibata and T. Susuki. 1996. An allele-specific abnormal transcript of the heat shock protein 70 gene in patients with major depression. Biochem. Biophys. Res. Commun. 219:745-752.
  20. Silvia, E., Valtorta and Miriam R. Gallardo. 2003. Evaporative cooling for Holstein dairy cows under grazing conditions. International Journal of Biometeorology 25:59-87.
  21. Villunger, A., E. M. Michalak and M. Coultas. 2003. p53 and drug-induced apoptitic response mediated by BH3-only proteins puma and noxa. Science 302:1036-1038.
  22. Yic, C., C. M. Knudson and S. J. Korsmeyer. 1997. Bax suppresses tumorigenesis and stimulates apoptosis in vivo. Nature 385:637-640.

Cited by

  1. A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows vol.38, pp.1, 2011, https://doi.org/10.1007/s11033-010-0080-8
  2. Feeding glycerol-enriched yeast culture improves performance, energy status, and heat shock protein gene expression of lactating Holstein cows under heat stress1 vol.92, pp.6, 2014, https://doi.org/10.2527/jas.2013-7152
  3. SNP of AHSA2 gene in three cattle breeds using snapshot technology vol.99, pp.1, 2020, https://doi.org/10.1007/s12041-020-01211-z