Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Genetic Analysis of Three River Populations of Catla catla (HAMILTON) Using Randomly Amplified Polymorphic DNA Markers

  • Islam, M.S. (Department of Biotechnology, Bangladesh Agricultural University) ;
  • Ahmed, A.S.I. (Department of Fisheries Biology and Genetics, Bangladesh Agricultural University) ;
  • Azam, M.S. (Department of Fisheries Biology and Genetics, Bangladesh Agricultural University) ;
  • Alam, M.S. (Department of Fisheries Biology and Genetics, Bangladesh Agricultural University)
  • Received : 2004.04.09
  • Accepted : 2004.10.04
  • Published : 2005.04.01
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Abstract

The genetic variations in three major river populations viz. the Halda, the Jamuna and the Padma of the Indian major carp, Catla catla were analyzed by Random Amplified Polymorphic DNA (RAPD) markers. Four decamer primers were used for amplifying DNA of 10 individuals from each population. The proportion of polymorphic loci and the gene diversity estimates were 59.4 and 0.20 for the Halda, 37.5 and 0.14 for the Jamuna and 46.9 and 0.16 for the Padma populations respectively indicating the existence of a relatively high level of genetic variation in the Halda river population. The inter-population similarity indices, gene flow and genetic distance values indicated that the Jamuna-Padma population pair of catla was genetically closer than the Halda-Jamuna and the Halda-Padma population pairs in compliance with the geographical distances among them. The coefficient of gene differentiation ($G_{ST}$=0.13) reflects some degree of genetic differentiation among three populations of catla studied. The data suggest that the RAPD technique could be used to discriminate different river populations of catla.

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