Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Effects of Seeding Microorganisms, Hydrazine, and Nitrite Concentration on the Anammox Activity

혐기성 암모늄 산화균의 활성에 대한 식종미생물, 히드라진 및 아질산성 질소 농도의 영향

  • Jung, Jin-Young (Water Environment & Remediation Research Center, KIST) ;
  • Kang, Shin-Hyun (Water Environment & Remediation Research Center, KIST) ;
  • Kim, Young-O (Water Environment & Remediation Research Center, KIST) ;
  • Chung, Yun-Chul (Water Environment & Remediation Research Center, KIST)
  • 정진영 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 강신현 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 김영오 (한국과학기술연구원 수질환경 및 복원연구센터) ;
  • 정윤철 (한국과학기술연구원 수질환경 및 복원연구센터)
  • Received : 2005.03.15
  • Accepted : 2005.05.17
  • Published : 2005.09.30
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Abstract

Anammox (Anaerobic Ammonium Oxidation) bacteria is recently discovered microorganism which can oxidize ammonium to nitrogen gas in the presence of nitrite under anaerobic conditions. The anammox process can save an energy for nitrification and need not require a carbon source for denitrification, however, the start-up periods takes a long time more than several months due to the long doubling time (approximately 11 days). In order to find the effects of seeding microorganisms, hydrazine, and nitrite concentration on the enhancement of the anammox activity, five kinds of microorganisms were selected. Among the several kinds of seeding microorganisms, the granule from acclimated microorganisms treating high concentration of ammonia nitrogen (A-1) and sludge from piggery wastewater treatment plant (A-2) were found to have a high anammox activity. In the case of A-1, the maximum nitrogen conversion rate represented 0.4 mg N/L-hr, and the amount of nitrite utilization was high compared to those of other seeding microorganisms. The A-4 represented a higher nitrogen conversion rate to 0.7 mg N/L-hr although the ammonium concentration in the serum bottle was high as 200 mg/L. Meanwhile, the anaerobic granule from UASB reactor treating distillery wastewater showed a low anammox activity due to the denitrification by the remained carbon sources in the granule. Hydrazine, intermediate product in anammox reaction, enhanced the anammox activity by representing 1.4 times of nitrogen gas was produced in the test bottle than that of control, when 0.4 mM of $N_2H_4$ was added to serum bottle which contains 5 mM of nitrite. The high concentration of nitrite (10 mM) resulted in the decrease of the anammox activity by showing lower production of nitrogen gas compared to that of 5 mM addition of nitrite concentration. As a result of FISH (Florescence In-Situ Hybridization) experiment, the Amx820 probe showed a more than 13% of anammox bacteria in a granule (A-1).

Keywords

Acknowledgement

Supported by : 환경부

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