Molecules and Cells

  • 제19권3호
  • /
  • Pages.445-451
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  • 2005
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
권호 표지

Mechanisms Underlying the Effects of LPS and Activation-induced Cytidine Deaminase on IgA Isotype Expression

  • Park, Seok-Rae (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Kim, Hyun-A (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Chun, Sung-Ki (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kim, Pyeung-Hyeun (Department of Microbiology, College of Natural Sciences, Kangwon National University)
  • 투고 : 2005.02.14
  • 심사 : 2005.04.06
  • 발행 : 2005.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

Activation-induced cytidine deaminase (AID) is needed for Ig class switch recombination (CSR). We explored the effect of LPS on the expression of AID during B cell differentiation, and the role of AID in IgA isotype expression. In normal spleen B cells, LPS increased AID transcription up to 48 h post-stimulation, i.e. around the time of Ig CSR. TGF-${\beta}1$ and AID were required for IgA expression, and LPS contributed to $TGF{\beta}1$-induced IgA production largely by inducing AID. Interestingly, LPS repressed AID transcription in $sIgA^+$ B cells but still stimulated IgA production mainly by increasing the rate of IgA secretion. Our data indicate that LPS contributes to $TGF{\beta}1$-induced IgA isotype expression in at least two ways: by stimulating AID transcription before CSR and by enhancing the IgA secretion rate after CSR.

키워드

과제정보

연구 과제 주관 기관 : Korea Science and Engineering Foundation

참고문헌

  1. Caivano, M. and Cohen, P. (2000) Role of mitogen-activated protein kinase cascades in mediating lipopolysaccharidestimulated induction of cyclooxygenase-2 and IL-1 beta in RAW264 macrophages. J. Immunol. 164, 3018-3025
  2. Castigli, E., Wilson, S. A., Scott, S., Dedeoglu, F., Xu, S., et al. (2005) TACI and BAFF-R mediate isotype switching in B cells. J. Exp. Med. 201, 35-39 https://doi.org/10.1084/jem.20032000
  3. Cazac, B. B. and Roes, J. (2000) TGF-beta receptor controls B cell responsiveness and induction of IgA in vivo. Immunity 13, 443-451 https://doi.org/10.1016/S1074-7613(00)00044-3
  4. Chaudhuri, J. and Alt, F. W. (2004) Class-switch recombination: interplay of transcription, DNA deamination and DNA repair. Nat. Rev. Immunol. 4, 541-552 https://doi.org/10.1038/nri1395
  5. Coffman, R. L., Lebman, D. A., and Shrader, B. (1989) Transforming growth factor beta specifically enhances IgA production by lipopolysaccharide-stimulated murine B lymphocytes. J. Exp. Med. 170, 1039-1044 https://doi.org/10.1084/jem.170.3.1039
  6. Dedeoglu, F., Horwitz, B., Chaudhuri, J., Alt, F. W., and Geha, R. S. (2004) Induction of activation-induced cytidine deaminase gene expression by IL-4 and CD40 ligation is dependent on STAT6 and NFkappaB. Int. Immunol. 16, 395-404 https://doi.org/10.1093/intimm/dxh042
  7. Durandy, A. (2003) Activation-induced cytidine deaminase: a dual role in class-switch recombination and somatic hypermutation. Eur. J. Immunol. 33, 2069-2073 https://doi.org/10.1002/eji.200324133
  8. Fugmann, S. D. and Schatz, D. G. (2003) RNA AIDs DNA. Nat. Immunol. 4, 429-430 https://doi.org/10.1038/ni0503-429
  9. Guha, M. and Mackman, N. (2001) LPS induction of gene expression in human monocytes. Cell. Signal. 13, 85-94 https://doi.org/10.1016/S0898-6568(00)00149-2
  10. Hambleton, J., Weinstein, S. L., Lem, L., and DeFranco, A. L. (1996) Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93, 2774-2778
  11. Honjo, T., Kinoshita, K., and Muramatsu, M. (2002) Molecular mechanism of class switch recombination: linkage with somatic hypermutation. Annu. Rev. Immunol. 20, 165-196 https://doi.org/10.1146/annurev.immunol.20.090501.112049
  12. Honjo, T., Muramatsu, M., and Fagarasan, S. (2004) AID: how does it aid antibody diversity? Immunity 20, 659-668 https://doi.org/10.1016/j.immuni.2004.05.011
  13. Islam, K. B., Nilsson, L., Sideras, P., Hammarstrom, L., and Smith, C. I. (1991) TGF-beta 1 induces germ-line transcripts of both IgA subclasses in human B lymphocytes. Int. Immunol. 3, 1099-1106 https://doi.org/10.1093/intimm/3.11.1099
  14. Iwasato, T., Shimizu, A., Honjo, T., and Yamagishi, H. (1990) Circular DNA is excised by immunoglobulin class switch recombination. Cell 62, 143-149 https://doi.org/10.1016/0092-8674(90)90248-D
  15. Kearney, J. F. and Lawton, A. R. (1975) B lymphocyte differentiation induced by lipopolysaccharide. I. Generation of cells synthesizing four major immunoglobulin classes. J. Immunol. 115, 671-676
  16. Kenter, A. L. (2003) Class-switch recombination: after the dawn of AID. Curr. Opin. Immunol. 15, 190-198 https://doi.org/10.1016/S0952-7915(03)00018-9
  17. Kim, P. H. and Kagnoff, M. F. (1990) Transforming growth factor beta 1 increases IgA isotype switching at the clonal level. J. Immunol. 145, 3773-3778
  18. Kim, P. H., Kim, H. T., Yoon, K. S., Yie, S. W., Kim, M. K., et al. (1994) Transforming growth factor-beta1 derived from murine peyer's patch T lymphocytes specifically increases IgA isotype synthesis. Mol. Cells 4, 171-176
  19. Kim, P. H., Eckmann, L., Lee, W. J., Han, W., and Kagnoff, M. F. (1998) Cholera toxin and cholera toxin B subunit induce IgA switching through the action of TGF-beta 1. J. Immunol. 160, 1198-1203
  20. Lebman, D. A., Park, M. J., Hansen-Bundy, S., and Pandya, A. (1994) Mechanism for transforming growth factor beta regulation of alpha mRNA in lipopolysaccharide-stimulated B cells. Int. Immunol. 6, 113-119 https://doi.org/10.1093/intimm/6.1.113
  21. Lee, S. C., Han, J. S., Seo, J. K., and Cha, Y. N. (2003) Modulation of cyclooxygenase-2 expression by phosphatidylcholine specific phospholipase C and D in macrophages stimulated with lipopolysaccharide. Mol. Cells 15, 320-326
  22. Li, S. C., Rothman, P. B., Zhang, J., Chan, C., Hirsh, D., et al. (1994) Expression of I mu-C gamma hybrid germline transcripts subsequent to immunoglobulin heavy chain class switching. Int. Immunol. 6, 491-497 https://doi.org/10.1093/intimm/6.4.491
  23. Min, K. M. and Kim, P. H. (2003) Macrophage-derived TGFbeta1 induces IgA isotype expression. Mol. Cells 16, 245- 250
  24. Muller, J. M., Ziegler-Heitbrock, H. W., and Baeuerle, P. A. (1993) Nuclear factor kappa B, a mediator of lipopolysaccharide effects. Immunobiology 187, 233-256
  25. Muramatsu, M., Sankaranand, V. S., Anant, S., Sugai, M., Kinoshita, K., et al. (1999) Specific expression of activationinduced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 274, 18470-18476 https://doi.org/10.1074/jbc.274.26.18470
  26. Muramatsu, M., Kinoshita, K., Fagarasan, S., Yamada, S., Shinkai, Y., et al. (2000) Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 102, 553-563 https://doi.org/10.1016/S0092-8674(00)00078-7
  27. Murray, P. D., McKenzie, D. T., Swain, S. L., and Kagnoff, M. F. (1987) Interleukin 5 and interleukin 4 produced by Peyer's patch T cells selectively enhance immunoglobulin A expression. J. Immunol. 139, 2669-2674
  28. Nakamura, M., Kondo, S., Sugai, M., Nazarea, M., Imamura, S., et al. (1996) High frequency class switching of an IgM+ B lymphoma clone CH12F3 to IgA+ cells. Int. Immunol. 8, 193-201 https://doi.org/10.1093/intimm/8.2.193
  29. Oliver, A. M., Martin, F., and Kearney, J. F. (1999) Ig- MhighCD21high lymphocytes enriched in the splenic marginal zone generate effector cells more rapidly than the bulk of follicular B cells. J. Immunol. 162, 7198-7207
  30. Park, S. R., Lee, J. H., and Kim, P. H. (2001) Smad3 and Smad4 mediate transforming growth factor-beta1-induced IgA expression in murine B lymphocytes. Eur. J. Immunol. 31, 1706-1715 https://doi.org/10.1002/1521-4141(200106)31:6<1706::AID-IMMU1706>3.0.CO;2-Z
  31. Roper, R. L., Conrad, D. H., Brown, D. M., Warner, G. L., and Phipps, R. P. (1990) Prostaglandin $E_{2}$ promotes IL-4-induced IgE and IgG1 synthesis. J. Immunol. 145, 2644-2651
  32. Shockett, P. and Stavnezer, J. (1991) Effect of cytokines on switching to IgA and alpha germline transcripts in the B lymphoma I.29 mu. Transforming growth factor-beta activates transcription of the unrearranged C alpha gene. J. Immunol. 147, 4374-4383
  33. Stavnezer, J. (2000) Molecular processes that regulate class switching. Curr. Top. Microbiol. Immunol. 245, 127-168
  34. van Ginkel, F. W., Wahl, S. M., Kearney, J. F., Kweon, M. N., Fujihashi, K., et al. (1999) Partial IgA-deficiency with increased Th2-type cytokines in TGF-beta 1 knockout mice. J. Immunol. 163, 1951-1957
  35. van Vlasselaer, P., Punnonen, J., and de Vries, J. E. (1992) Transforming growth factor-beta directs IgA switching in human B cells. J. Immunol. 148, 2062-2067
  36. Woo, C. H., Lim, J. H., and Kim, J. H. (2004) Lipopolysaccharide induces matrix metalloproteinase-9 expression via a mitochondrial reactive oxygen species-p38 kinase-activator protein-1 pathway in Raw 264.7 cells. J. Immunol. 173, 6973-6980
  37. Ye, X. and Liu, S. F. (2001) Lipopolysaccharide regulates constitutive and inducible transcription factor activities differentially in vivo in the rat. Biochem. Biophys. Res. Commun. 288, 927-932 https://doi.org/10.1006/bbrc.2001.5883
  38. Zhou, C., Saxon, A., and Zhang, K. (2003) Human activationinduced cytidine deaminase is induced by IL-4 and negatively regulated by CD45: implication of CD45 as a Janus kinase phosphatase in antibody diversification. J. Immunol. 170, 1887-1893