Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Mechanisms Underlying the Effects of LPS and Activation-induced Cytidine Deaminase on IgA Isotype Expression

  • Park, Seok-Rae (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Kim, Hyun-A (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Chun, Sung-Ki (Department of Microbiology, College of Natural Sciences, Kangwon National University) ;
  • Park, Jae-Bong (Department of Biochemistry, College of Medicine, Hallym University) ;
  • Kim, Pyeung-Hyeun (Department of Microbiology, College of Natural Sciences, Kangwon National University)
  • Received : 2005.02.14
  • Accepted : 2005.04.06
  • Published : 2005.06.30
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Abstract

Activation-induced cytidine deaminase (AID) is needed for Ig class switch recombination (CSR). We explored the effect of LPS on the expression of AID during B cell differentiation, and the role of AID in IgA isotype expression. In normal spleen B cells, LPS increased AID transcription up to 48 h post-stimulation, i.e. around the time of Ig CSR. TGF-${\beta}1$ and AID were required for IgA expression, and LPS contributed to $TGF{\beta}1$-induced IgA production largely by inducing AID. Interestingly, LPS repressed AID transcription in $sIgA^+$ B cells but still stimulated IgA production mainly by increasing the rate of IgA secretion. Our data indicate that LPS contributes to $TGF{\beta}1$-induced IgA isotype expression in at least two ways: by stimulating AID transcription before CSR and by enhancing the IgA secretion rate after CSR.

Keywords

Acknowledgement

Supported by : Korea Science and Engineering Foundation

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