Mechanism of Anti-inflammatory Effect of Peucedanum japonicum Thunb

방풍의 항염 효과 기전

  • Noh, Sung-Il (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Kim, Sang-Don (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Park, Sung-Cheul (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Seo, Byung-Yun (Dept. of Third Medicine, Professional Graduate School of Oriental Medicine, Won-Kwang University) ;
  • Yeom, Seung-Ryong (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Kwon, Young-Dal (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Shin, Byung-Cheul (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University) ;
  • Song, Yung-Sun (Dept. of Oriental Rehabilitation Medicine, Collate of Oriental Medicine, Won-Kwang University)
  • 노성일 (원광대학교 한의과대학 한방재활의학교실) ;
  • 김상돈 (원광대학교 한의과대학 한방재활의학교실) ;
  • 박성철 (원광대학교 한의과대학 한방재활의학교실) ;
  • 서병윤 (원광대학교 한의학전문 대학원 제3의학과) ;
  • 염승룡 (원광대학교 한의과대학 한방재활의학교실) ;
  • 권영달 (원광대학교 한의과대학 한방재활의학교실) ;
  • 신병철 (원광대학교 한의과대학 한방재활의학교실) ;
  • 송용선 (원광대학교 한의과대학 한방재활의학교실)
  • Received : 2005.03.24
  • Accepted : 2005.04.20
  • Published : 2005.04.30

Abstract

목적 : 방풍은 임상적으로 관절염을 포함한 다양한 염증성 질환 치료제로 사용되어 왔다. 본 연구에서는 인간 비만세포를 이용하여 세포 독성에 영향을 주지 않는 농도에서 방풍의 항염 효과 및 그 기전을 검토했다. 방법 : 인간의 HMC-1세포를 IMDM에서 페니실린, 스트렙토마이신, 모노티오글리세린을 첨가하여 배양하고 방품추출액을 투여하였다. 그 다음 MTT, CLISA, RT-PCR, 세포내 칼슘측정, 핵단백분석을 이용하여 TNF-${\alpha}$, IL-6, IL-8 각각의 형성과 mRNA발현, 세포내 칼슘 수준, NF-${\kappa}B$ 발현에 대한 방풍추출액의 반응을 측정하고 통계처리 하였다. 결과 : 방풍은 PMA와 calcium ionophore A23187로 활성화된 비민세포에서 세포내 칼슘 수준과 NF-${\kappa}B$, TNF-${\alpha}$와 IL-6의 발현을 억제시켰고 RT-PCR을 이용한 mRNA 발현에서 TNF-${\alpha}$와 IL-6의 발현을 억제하였다. 결론 : 방풍은 비만세포내 칼슘 수준 및 NF-${\kappa}B$의 활성을 억제하고 염증성 세포 활성 물질인 TNF-${\alpha}$와 IL-6의 분비도 억제하여 항염효과를 나타냄을 암시하고 있다.

Keywords

References

  1. J Vet Sci. v.3 no.4 The antinociceptive and anti-inflammatory effect of ethylacetate extracts from Bang-Poong(Radix ledebouriellae) on the Freund's adjuvant-induced arthritis in rats Kim, H.W.;Kwon, Y.B.;Ham, T.W.;Roh, D.H.;Yoon, S.Y.;Han, H.J.;Kang, S.K.;Lee, H.J.;Mar, W.C.;Yang, I.S.;Beitz, A.J.;Lee, J.H.
  2. Am J Chin Med. v.29 no.2 Suppression of collagen-induced arthritis in rats by continuous administration of dae-bang-poong-tang (da-fang-feng-tang) Joe, S.M.;Lee, I.S.;Lee, Y.T.;Lee, J.H.;Choi, B.T.
  3. N Engl J Med. v.328 no.4 New concepts about the mast cell Galli, S.J.
  4. Immunol Rev. v.173 Mast cells in innate immunity Mekori, Y.A.;Metcalfe, D.D.
  5. Front Biosci. v.6 The human mast cell: functions in physiology and disease Krishnaswamy, G.;Kelley, J.;Johnson, D.;Youngberg, G.;Stone, W.;Huang, S.K.;Bieber, J.;Chi, D.S.
  6. Crit Rev Oncol Hematol. v.31 no.2 Immunopathology and human mast cell cytokines Bradding, P.;Holgate, S.T.
  7. J Exp Med. v.194 no.1 The diverse roles of mast cells Gurish, M.F.;Austen, K.F.
  8. N Engl J Med. v.344 no.1 Allergy and allergic diseases Kay, A.B.
  9. Cold. Spring. Harb. Symp. Quant. Biol. v.51 Tumor necrosis factors: gene structure and biological activities Goeddel, D.V.;Aggarwal, B.B.;Gray, P.W.;Leung, D.W.;Nedwin, G.E.;Palladino, M.A.;Patton, J.S.;Pennica, D.;Shepard, H.M.;Sugarman, B.J.
  10. Exp Dermatol. v.9 no.2 Inhibition of cytokine secretion from human leukemic mast cells and basophils by H1- and H2-receptor antagonists Lippert, U.;Moller, A.;Welker, P.;Artuc, M.;Henz, B.M.
  11. Cytokines in animal health and disease Lederer, J.A.;Czuprynski, C.J.;Myers, M.J.;Murtaugh, M.P.
  12. Proc. Natl. Acad. Sci. USA. v.86 Interlukin-6 is expressed in high levels in psoriatic skin and stimulates proliferation of cultured human kertinocytes Grossman, R.M.;Krueger, J.;Yourish, D.;Granelli-Peperno, A.;Murphy, D.P.;May, L.T.;Kupper, T.S.;Sehgal, P.B.;Gottlieb, A.B.
  13. J. Immunol. v.152 IL-6-anti-IL-6 auto-antibody complexes with IL-6 activity in sera from some patients with systemic sclerosis Suzuki, H.;Takemura, H.;Yoshizaki. K.;Koishihara, Y.;Ohsugi, Y.;Okano, A.;Akiyama, Y.;Tojo, T.;Kishimoto, T.;Kashiwagi, H.
  14. Ann. N. Y. Acad. Sci. v.389 Biosynthesis of C-reactive protein Kushner, I.;Macintyre, S.S.;Schultz, D.
  15. Z. Zellforsch. v.121 Die zytochemische identification der mastzellvorstufen bei der ratte Desaga, J.F.;Parwareserch, M.R.;Muller-Hermeling, H.K.
  16. Proc. Natl. Acad. Sci. USA v.85 Selective differenciation and prolifeation of hematopoietic cells induced by recombinant human interleukins Satio, H.;Hatake, K.;Dvorak, A.M.;Leiferman, K.M.;Dannenberg, A.D.;Arai, N.;Ishizaka, K.;Ishizaka, T.
  17. J. Invest. Dermatol. v.103 Comparative cytokine release from human monocytes, monocyte-derived immature mast cells, anda human mast cell line (HMC-1) Grabbe, J.;Welker, P.;Moller, A.;Dippel, E.;Ashman, L.K.;Czarnetzki, B.M.
  18. Physiol Rev. v.57 no.3 Relationships between calcium and cyclic nucleotides in cell activation Rasmussen, H.;Goodman, D.B.
  19. Proc Natl Acad Sci U S A v.82 no.23 Antigen-induced increase in protein kinase C activity in plasma membrane of mast cells White, J.R.;Pluznik, D.H.;Ishizaka, K.;Ishizaka, T.
  20. Physiol Rev. v.77 no.4 Mast cells Metcalfe, D.D.;Baram, D.;Mekori, Y.A.
  21. Ann Intern Med. v.128 no.2 The pathophysiologic roles of interleukin-6 human disease Papanicolaou, D.A.;Wilder, R.L.;Manolagas, S.C.;Chrousos, G.P.
  22. J Allergy Clin Immunol. v.105 no.3 Dependence of mast cell lgE-mediated cytokine production on nuclear factor-kappaB activity Marquardt, D.L.;Walker, L.L.
  23. J Pathol. v.189 no.2 Immunohistochemical analysis of the activation of NF-kappaB and expression of associated cytokines and adhesion molecules in human models of allergic inflammation Wilson, S.J.;Leone, B.A.;Anderson, D.;Manning, A.;Holgate, S.T.
  24. Proc Natl Acad Sci U S A v.92 no.23 Stimulation-dependent I kappa B alpha phosphorylation marks the NF-kappa B inhibitor for degradation via the ubiquitin-proteasome pathway Alkalay, I.;Yaron, A.;Hatzubai, A.;Orian, A.;Ciechanover, A.;Ben-Neriah, Y.
  25. J Immunol. v.126 Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells Schwartz, L.B.;Lewis, R.A.;Seldin, D.;Austen, K.F.
  26. J Allergy Clin Immunol. v.103 Role of cyclic 3'.5'-adensine monophosphate in the regulation of chemical mediator release and cytokine production from cultured human mast cells Shichijo, M.;Inagaki, M.;Kimata, M.;Serizawa, I.;Saito, H.;Nagai, H.
  27. J Invest Dermatol. v.103 Comparative cytokine release from human monocytes, monocyte-derived immature mast cells and a human mast cell line(HMC-1) Grabbe, J.;Welker, P.;Moller, A.;Dippel, E.;Ashman, L.K.;Czarnetzki, B.M.
  28. Invest Ophthalmol Vis Sci. v.39 Isolation of human conjunctival mast cells and epithelial cells: Tumor necrosis factor-${\alpha}$ from mast cells affects intercellular adhesion molecule-1 expression on epithelial cells Cook, E.B.;Stahl, J.L.;Miller, S.T.;Gern, J.E.;Sukow, K.A.;Graziano, F.M.;Barney, N.P.
  29. Int Arch Allergy Immunol. v.132 no.3 Azelastine inhibits secretion of IL-6, TNF-alpha and IL-8 as well as NF-kappaB activation and intracellular calcium ion levels in normal human mast cells Kempuraj, D.;Huang, M.;Kandere-Grzybowska, K.;Basu, S.;Boucher, W.;Letourneau, R.;Athanassiou, A.;Theoharides, T.C.
  30. J Allergy Clin Immunol. v.90 The antiallergic effects of antihistamines ($H_1$_receptor antagonists) stelle, F.;Simons, R.
  31. Inflamm Res. v.49 no.7 In vitro inhibitory effect of rupatadine on histamine and TNF-alpha release from dispersed canine skin mast cells and the human mast cell line HMC-1 Queralt, M.;Brazis, P.;Merlos, M.;de Mora, F.;Puigdemont, A.
  32. J Immunol. v.159 no.6 Suppression of TNF-alpha secretion by azelastine in a rat mast (RBL-2H3) cell line: evidence for differential regulation of TNF-alpha release, transcription, and degranulation Hide, I.;Toriu, N.;Nuibe, T.;Inoue, A.;Hide, M.;Yamamoto, S.;Nakata, Y.
  33. Eur J Pharmacol. v.322 no.1 Effects of the anti-allergics astemizole and no-rastemizole on Fc epsilon RI receptor-mediated signal transduction processes Fischer, M.J.;Paulussen, J.J.;Kok-Van Esterik, J.A.;Van der Heijden, V.S.;De Mol, N.J.;Janssen, L.H.
  34. J Immunol. v.157 no.1 Selective growth of human mast cells induced by Steel factor, IL-6, and prostaglandin E2 from cord blood mononuclear cells Saito, H.;Ebisawa, M.;Tachimoto, H.;Shichijo, M.;Fukagawa, K.;Matsumoto, K.;Iikura, Y.;Awaji, T.;Tsujimoto, G.;Yangida, M.;Uzumaki, H.;Takahashi, G.;Tsuji, K.;Nakahata, T.
  35. Biochem Biophys Res Commun. v.257 no.3 $Ca^{2+}$ and protein kinase C signaling for histamine and sulfidoleukotrienes released from human cultured mast cells Kimata, M.;Shichijo, M.;Miura, T.;Serizawa, I.;Inagaki, N.;Nagai, H.
  36. J Biol Chem. v.259 no.11 The mechanism of the calcium signal and correlation with histamine release in 2H3 cells Beaven, M.A.;Rogers, J.;Moore, J.P.;Hesketh, T.R.;Smith, G.A.;Metcalfe, J.C.
  37. Planta Med. v.66 no.1 Effects of luteolin and other flavonoids on IgE-mediated allergic reactions Kimata, M.;Inagaki, N.;Nagai, H.
  38. Biochem Soc Symp. no.39 Involvement of calcium in exocytosis and the exocytosis-vesiculation sequence Douglas, W.W.
  39. N Engl J Med. v.336 Nuclear factor-kB-Apivotal transcription factor in chronic inflammatory diseases Barnes, P.J.;Karin, M.
  40. Blood. Purif. v.19 Inflammatory and atherosclerotic interactions in tepleted uremic patients Stenvinkel, P.